ELISA Kits

Showing 301–450 of 3623 results

  • The CB1 receptor is a G-protein coupled receptor that binds the active component of cannabis, Δ9-tetrahydrocannabinol. This antibody has been raised against the C-terminal (amino acids 461-472) intracellular region of the human CB1 receptor and can be used for western blotting.{6749,6747} Human and rat CB1 receptors exhibit 97.3% homology at the amino acid level over the complete protein, and 100% homology within the peptide sequence used to make this antibody.{6629,6628} This peptide exhibits no homology with the CB2 receptor. Based on the amino acid sequence, the CB1 receptor has a molecular weight of approximately 52,800.{6628} The CB1 receptor and the splice variant CB1a are localized mainly in the brain, whereas the CB2 receptor is localized predominantly in peripheral tissues, including the spleen and hemopoietic cells.{6629,6628,6726,6727}  

     

    Brand:
    Cayman
    SKU:10006590 - 1 ea

    Available on backorder

  • The CB1 receptor and the splice variant CB1a are localized mainly in the brain.{6628,6726,6724} The CB1 receptor is a G-protein coupled receptor that binds the active component of cannabis, Δ9-tetrahydrocannabinol. Human and rat CB1 receptors exhibit 97.3% homology at the amino acid level over the complete protein, and 100% homology within the peptide sequence used to make this antibody.{6629,6628} This peptide exhibits no homology with the CB2 receptor. Based on the amino acid sequence, the CB1 receptor has a molecular weight of approximately 52,800.{6628} The CB1 receptor and the splice variant CB1a are localized mainly in the brain, whereas the CB2 receptor is localized predominantly in peripheral tissues, including the spleen and hemopoietic cells.{6629,6628,6726,6724} Cayman’s CB1 Receptor Polyclonal Antibody can be used for Western blot and immunohistochemistry applications. The antibody recognizes the N-terminal extracellular region of the CB1 receptor at 40 kDa from human samples.  

     

    Brand:
    Cayman
    SKU:101500 - 1 ea

    Available on backorder

  • The CB2 receptor is localized predominantly in peripheral tissues, including the spleen and hemopoietic cells.{6724} · The CB1 and CB2 receptors are G-protein coupled receptors that bind the active component of cannabis, Δ9-tetrahydrocannabinol, as well as anandamide which is an endogenous CB receptor ligand. This antibody has been raised against a sequence between the N-terminus and the first transmembrane domain of the protein of the human CB2 receptor.{6724} It can be used for western blotting and immunohistochemistry applications. Conserved amino acids between the CB1 and CB2 receptors in this region are minimal.{6727} Human and murine CB2 receptors exhibit 82% homology at the amino acid level over the complete protein.{6727} The CB2 receptor is localized predominantly in peripheral tissues, including the spleen and hemopoietic cells.{6724}  

     

    Brand:
    Cayman
    SKU:101550 - 1 ea

    Available on backorder

  • The central cannabinoid (CB1) and peripheral cannabinoid (CB2) receptors are G protein-coupled receptors (GPCRs) that bind the active component of cannabis, Δ9-tetrahydrocannabinol, as well as anandamide which is an endogenous CB receptor ligand. This antibody has been raised against a sequence between the N-terminal and the first transmembrane domain of the protein of the human CB2 receptor.{6724} Conserved amino acids between the CB1 and CB2 receptors in this region are minimal.{6727} Human and mouse CB2 receptors exhibit 82% homology at the amino acid level over the complete protein.{6727} The CB2 receptor is localized predominantly in peripheral tissues, including the spleen and hemopoietic cells.{6724}  

     

    Brand:
    Cayman
    SKU:10010712 - 500 µl

    Available on backorder

  • CCL2 also known as the monocyte chemoattractant protein-1 (MCP-1) is a chemokine and more particularly an C-C chemokine. Initially discovered in 1983, it is a monomeric polypeptide of 73 amino acids (13 kDa). It belongs to a family composed at least of members (MCP-1, -2, -3, and -4). CCL-2 is produced by the immune-cells (monocyte/macrophages) and non-immune cells (endothelial cells-fibroblast-myocyte). The main function of the CCL2 is the chimiotactism and more particularly the regulation of the migration and the infiltration of monocytes, memory T lymphocytes, and natural killer (NK) cells on an infection site. CCL2 is involved in many diseases like HIV-1 pathogenesis, cancer, cardiovascular disease, or diabetes. [Bertin Catalog No. A05415]  

     

    Brand:
    Cayman
    SKU:23621 - 96 wells

    Available on backorder

  • CD146, also known as the melanoma cell adhesion molecule (MCAM), is an adhesion molecule and type 1 transmembrane glycoprotein with roles in vascular homeostasis, cell migration, and neuronal differentiation and is encoded by MCAM in humans.{58076,58077} It is comprised of a signal sequence, an extracellular domain containing five immunoglobulin-like (Ig-like) domains, a hydrophobic transmembrane domain, and a cytosolic domain composed of an ezrin, radixin, moesin (ERM) protein complex binding motif and a double leucine motif for epithelial basolateral targeting. Alternative splicing of MCAM produces two isoforms that differ in length of their cytosolic domain, CD146 short (shCD146) and CD146 long (lgCD146), that contain one or two protein kinase C (PKC) phosphorylation sites, respectively.{58078} LgCD146 is expressed on endothelial cells, smooth muscle cells (SMCs), and pericytes where it is localized to tight junctions, as well as in mononuclear cells, mesenchymal stromal cells, and trophoblasts.{58076} It induces stabilization of capillary-like structures in a Matrigel™ assay. Cayman’s CD146 Long Rabbit Monoclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications.  

     

    Brand:
    Cayman
    SKU:32202 - 100 µl

    Available on backorder

  • Revealing the molecular signals involved in B and T cell activation is critical to the future discovery of therapeutics that can augment cell-mediated immune responses to disease.{12747,12749,12748} CD150 is a type I transmembrane glycoprotein that is involved in signal transduction of antigen specific responses in B and T cells. It is expressed by activated B cells isolated from tonsils and peripheral blood, B cells in germinal centers and mantle zone of lymph nodes, immature thymocytes, and CD45RO+CD45RA- subpopulation of T cells.{12746,12772} This antibody has been used to detect CD150 in neoplastic cells from diffuse large B-cell lymphoma, Hodgkin’s disease, hairy cell leukemia, and some tumors of ectodermal origin.{12771}  

     

    Brand:
    Cayman
    SKU:10004804 - 1 ea

    Available on backorder

  • CD20 is a non-glycosylated protein encoded by MS4A1 in humans.{59585} It is comprised of four transmembrane domains, a single intracellular loop, and two extracellular loop domains with both the N and C-termini located in the cytosol. CD20 is a general B cell marker that is expressed from the late pre-B lymphocyte stage but is not expressed by pro-B lymphocytes and is lost in terminally differentiated plasma cells and plasmablasts. It forms supramolecular complexes with CD53, CD81, and CD82, as well as MHCII, CD40, B cell receptors, and C-terminal Src kinase-binding protein (CBP) to contribute to signal transduction. MS4A1 expression is variable in B cell malignancies, with the lowest expression found in patients with chronic lymphocytic leukemia (CLL) and the highest expression found in patients with diffuse large B cell lymphoma (DLBCL) or hairy cell lymphomas. MS4A1 expression is enriched on IFN-γ-inducible T-box transcription factor-expressing B cells in blood isolated from patients with multiple sclerosis.{59586} Cayman’s CD20 (C-Term) Rabbit Monoclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications.  

     

    Brand:
    Cayman
    SKU:32222 - 100 µl

    Available on backorder

  • Interleukin-2 (IL-2) elicits its effects by binding to a multi-subunit cell-surface receptor that is inducible by various mitogens.{18182,18183,18184} The highest affinity for IL-2 is achieved when all 3 subunits (IL-2Rα or CD25, IL-2Rβ or CD122, and IL-2Rγ or CD132) are expressed together forming a heterotrimeric complex{6128}. CD25 is inducible in T-cells by antigen, mitogen or antibodies bound to the T-cell receptor CD3.{18183,18185} Binding of IL-2 to IL-2R on NK cells results in their conversion to LAK cells, which release several other cytokines (TNF, INF-γ, and GM-CSF).{6128,6349} Activated T-cells will respond to IL-2 by proliferating and secreting cytokines and cytolytic molecules. Activated B cells will proliferate and secrete antibodies.{11282}  

     

    Brand:
    Cayman
    SKU:10339 - 1 ea

    Available on backorder

  • CD27 is a type II homodimeric transmembrane glycoprotein (Mr = 55 kDa) and a member of the nerve growth factor/tumor necrosis receptor superfamily.{13302,13303} The antigen was identified on medullar thymocytes, mature T lymphocytes (CD45RA+CD45RO- T-cell subpopulation), memory B cells, plasma, and NK cells. This antibody is well suited for immunohistochemical detection of CD27 in B cell non-Hodgkin’s lymphoma samples.  

     

    Brand:
    Cayman
    SKU:10004837 - 1 ea

    Available on backorder

  • The CD3 antigen is a specific T-cell marker expressed on thymocytes and mature T-lymphocytes. The CD3 complex contains CD3g (Mr = 26 kDa), CD3d (Mr = 21 kDa), and CD3e (Mr = 20 kDa).{16550} CD3 is detected on the surface membrane or in the cytoplasm of T-cells from peripheral blood, depending on the state of cell differentiation. In addition to detection of T-cells from peripheral blood, the CD3 antibody can be used for detection of reactive T-cells in lymphoid infiltrates in tissues and neoplastic T-cells in non-Hodgkin’s lymphomas of T-cell lineage.{12060,16551}  

     

    Brand:
    Cayman
    SKU:10231 - 1 ea

    Available on backorder

  • CD31, also known as platelet endothelial cell adhesion molecule (PECAM-1), is a vascular cell adhesion molecule and member of the immunoglobulin (Ig) superfamily of glycoproteins.{54427} It is a transmembrane glycoprotein that contains a large extracellular domain with Ig homology domains and glycosaminoglycan binding sites, a membrane-spanning region, and a cytoplasmic domain containing multiple phosphorylation sites.{54427,54428} CD31 is expressed on a variety of cells, including endothelial cells, monocytes, neutrophils, and platelets, among others.{54427} It forms homophilic interactions, which allow it to aggregate at endothelial cell-cell junctions where it regulates vascular permeability.{54428} Tyrosine phosphorylation at sites on the CD31 cytoplasmic domain, including at immunoreceptor tyrosine-based inhibitory motifs (ITIMs), recruits cytoplasmic proteins, leading to downstream signaling with either inhibitory or activating effects.{54429} CD31 also has roles in integrin-mediated adhesion, leukocyte recruitment, angiogenesis, and inflammation.{54428,54430,54431} High levels of CD31+ circulating cells positively correlate with C-reactive protein (CRP) levels and the Framingham risk score for hard coronary artery disease, while low levels on circulating cells negatively correlate with the Framingham risk score.{54432} A mutation in PECAM1, the gene encoding CD31, is associated with atherosclerotic cerebral infarction.{54433} Cayman’s CD31 Rabbit Monoclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes CD31 at approximately 130 kDa from human samples.  

     

    Brand:
    Cayman
    SKU:32201 - 100 µl

    Available on backorder

  • CD34 is a type I transmembrane glycoprotein (Mr = 116 kDa). The antigen is expressed on stem cells and early hematopoietic progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts, and neurons.{13306,13307} This anti-CD34 monoclonal antibody can be used for the differential staining of acute leukemia cells.  

     

    Brand:
    Cayman
    SKU:10004835 - 1 ea

    Available on backorder

  • This CD34 monoclonal antibody recognizes an extracellular carbohydrate epitope of CD34.{15992} CD34 is a type I transmembrane glycoprotein (Mr = 104-120 kDa) expressed on stem cells, early hematopoietic progenitor cells, bone marrow stroma cells, endothelial cells, embryonic fibroblasts, and neurons.{13306,13307} CD34 analysis can be used for the differential identification of acute leukemias as well as routine analysis of cultured stem cell lines.  

     

    Brand:
    Cayman
    SKU:10208 - 1 ea

    Available on backorder

  • CD36 is a type-B scavenger receptor that is necessary for the formation of foam cells and thereby atherosclerotic lesions.{7719,9800,9801,9795} This membrane glycoprotein can internalize fatty acids which activate PPARγ and stimulate further expression of CD36.{9798,8499} This positive feedback loop combined with the murine CD36 knock-out studies reinforces the importance of CD36 in lipid metabolism. Additionally, CD36 is needed for the phagocytosis of P. falciparum infected erythrocytes, retinal pigment epithelial cell photoreceptor fragments, and post-apoptotic monocytes and neutrophils.{9794,9310,9793,9791} CD36 expression has been monitored during hematopoietic cell differentiation and may be an indicator of tumor spreading in lymphocytic leukemia.{9797} Positive controls include cells from adipose and heart tissue, platelets, and macrophages. Cayman Chemical’s CD36 Monoclonal Antibody (Clone JC63.1) is recommended for flow cytometry on CD36-containing cells of murine, rat, and human origin. The antibody can also be used to antagonize CD36-mediated cellular activity. Functional blocking inhibits CD36 binding of oxLDL (macrophages) and prevents foam cell formation of macrophages.  

     

    Brand:
    Cayman
    SKU:188150 - 100 µg

    Available on backorder

  • CD36 is a type-B scavenger receptor that is necessary for the formation of foam cells and thereby atherosclerotic lesions.{7719,9800,9801,9795} This membrane glycoprotein can internalize fatty acids which activate PPARγ and stimulate further expression of CD36.{9798,8499} This positive feedback loop combined with the murine CD36 knock-out studies reinforces the importance of CD36 in lipid metabolism. Additionally CD36 is needed for the phagocytosis of P. falciparum infected erythrocytes, retinal pigment epithelial cell photoreceptor fragments and post-apoptotic monocytes and neutrophils.{9794,9310,9793,9791} CD36 expression has been monitored during hematopoietic cell differentiation and may be an indicator of tumor spreading in lymphocytic leukemia.{9797} Positive controls include adipose and heart tissue, platelets, and macrophages. Cayman’s CD36 Monoclonal Antibody (Clone JC63.1) (azide free) can be used for flow cytometry, functional blocking, and immunocytochemistry applications.  

     

    Brand:
    Cayman
    SKU:10009893 - 100 µg

    Available on backorder

  • CD36 is a type-B scavenger receptor that is necessary for the formation of foam cells and thereby atherosclerotic lesions.{7719,9800,9801,9795} This membrane glycoprotein can internalize fatty acids which activate PPARγ and stimulate further expression of CD36.{9798,8499} This positive feedback loop combined with the murine CD36 knock-out studies reinforces the importance of CD36 in lipid metabolism. Additionally CD36 is needed for the phagocytosis of P. falciparum infected erythrocytes, retinal pigment epithelial cell photoreceptor fragments and post-apoptotic monocytes and neutrophils.{9794,9310,9793,9791} CD36 expression has been monitored during hematopoietic cell differentiation and may be an indicator of tumor spreading in lymphocytic leukemia.{9797} Positive controls include adipose and heart tissue, platelets, and macrophages. Cayman’s CD36 Monoclonal Antibody (Clone JC63.1) (azide free) can be used for flow cytometry, functional blocking, and immunocytochemistry applications.  

     

    Brand:
    Cayman
    SKU:10009893 - 500 µg

    Available on backorder

  • CD36 is a type-B scavenger receptor that is necessary for the formation of foam cells and thereby atherosclerotic lesions.{7719,9800, 9801,9795} This membrane glycoprotein can internalize fatty acids which activate proliferator-activated receptor γ and stimulate further expression of CD36.{9798,8499} This positive feedback loop combined with the murine CD36 knock-out studies reinforces the importance of CD36 in lipid metabolism. Additionally CD36 is needed for the phagocytosis of P. falciparum infected erythrocytes, retinal pigment epithelial cell photoreceptor fragments and post-apoptotic monocytes and neutrophils.{9794,9310,9793,9791} CD36 expression has been monitored during hematopoietic cell differentiation and may be an indicator of tumor spreading in lymphocytic leukemia.{9797} Positive controls include adipose and heart tissue, platelets, and macrophages. Cayman’s CD36 Monoclonal FITC Antibody (Clone JC63.1) can be used for flow cytometry and immunofluorescence applications.  

     

    Brand:
    Cayman
    SKU:10009870 - 1 ea

    Available on backorder

  • CD36 is a type-B scavenger receptor that is necessary for the formation of foam cells in atherosclerotic lesions.{7719,9800,9801,9795} This membrane glycoprotein can internalize fatty acids which activate PPARγ and stimulate further expression of CD36.{9798,8499} This positive feedback loop combined with the murine CD36 knock-out studies reinforces the importance of CD36 in lipid metabolism. Additionally CD36 is needed for the phagocytosis of P. falciparum infected erythrocytes, retinal pigment epithelial cell photoreceptor fragments, and post-apoptotic monocytes and neutrophils.{9794,9310,9793,9791} CD36 expression has been monitored during hematopoietic cell differentiation and may be an indicator of tumor spreading in lymphocytic leukemia.{9797} This transmembrane glycoprotein is detected by immunoblotting at 88 kDa or 54 kDa depending on the degree of glycosylation of the receptor in your sample. Positive controls include adipose and heart tissue, platelets, and macrophages.  

     

    Brand:
    Cayman
    SKU:100011 - 1 ea

    Available on backorder

  • CD37 is a type III transmembrane glycoprotein (Mr = 40-52 kDa). The leukocyte-specific protein CD37 is a member of the tetraspanin superfamily, originally described as a cell surface glycoprotein expressed on mature human B cells, but not on pro-B cells or plasma cells.{13049,13050} T cells and monocytes express CD37 at low levels. In humans, CD37 expression is restricted to B and T lymphocytes, monocytes, macrophages, neutrophils, dendritic cells, and certain leukocyte-derived malignant cells. CD37 is not expressed by NK cells, platelets, or erythrocytes.{13048} CD37 monoclonal antibody is recommended for immunophenotyping of B cell neoplasia (B cell chronic lymphocytic leukemia, hairy cell leukemia, and B cell non-Hodgkin’s lymphomas). Human, rat, and murine CD37 sequences have been identified.{13051,13052}  

     

    Brand:
    Cayman
    SKU:10004718 - 1 ea

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  • CD4 is a transmembrane glycoprotein present on the surface of a majority of thymocytes and T cell subpopulations, which recognize antigens presented by MHC class II molecules. The CD4 antigen is also found on monocytes, dendritic cells, tissue macrophages, and granulocytes. CD4+ T lymphocytes are presented by functional T-helper cells and CD4+CD25+ regulatory (suppressor) T cells. Estimation of CD4+ T cell level could be used for immunological status monitoring.{12087}  

     

    Brand:
    Cayman
    SKU:10004599 - 1 ea

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  • CD4, a transmembrane glycoprotein, is present on the surface of a majority of thymocytes and T cell subpopulations, which recognize antigens presented by MHC class II molecules. The CD4 antigen is also found on monocytes, dendritic cells, tissue macrophages and granulocytes. CD4+ T lymphocytes are presented by functional T-helper cells and CD4+CD25+ regulatory (suppressor) T cells. Estimation of CD4+ T cell level could be used for immunological status monitoring.{12087}  

     

    Brand:
    Cayman
    SKU:10010530 - 1 ea

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  • CD44 is a type I transmembrane glycoprotein and cell adhesion molecule belonging to the cartilage link protein family with roles in cell-cell and cell-matrix interactions, signal transduction, and cell migration.{55241,55242,55243} It is comprised of an N-terminal extracellular globular domain with a link domain that binds to the extracellular matrix component hyaluronan (HA) and other glycosaminoglycans, a membrane-proximal stem structure, a transmembrane domain, and a cytoplasmic domain that facilitates interactions with cytoskeleton-associated proteins.{55241,55244} The stem structure of CD44 can be modified via inclusion of variant exons between amino acid residues 222 and 223, resulting in several variant (CD44v) isoforms with the shortest and most prevalent isoform, known as the standard isoform (CD44s) or hematopoietic isoform (CD44H), having no exon insertions at this position.{55241,55245,55246} CD44 is expressed in a variety of cell types, including endothelial and epithelial cells, fibroblasts, and leukocytes.{55242} Binding of CD44 to HA is induced in T cells and monocytes upon antigen recognition and activation by inflammatory stimuli, respectively.{55247} The interaction between CD44 and HA mediates T cell rolling and recruitment of leukocytes to sites of inflammation. Several CD44 isoforms are expressed in tumor cells, including CD44v6, the overexpression of which is associated with tumor differentiation and lymph node metastasis in patients with non-small cell lung cancer (NSCLC).{55248,55249} Cayman’s CD44 Extracellular Domain Rabbit Monoclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications.  

     

    Brand:
    Cayman
    SKU:32214 - 100 µl

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  • CD45 is a type I transmembrane glycoprotein that is encoded by the PTPRC gene in humans and is abundantly expressed on nucleated hematopoietic cells.{49671} It contains an extracellular region that binds to glycoproteins and exists in various isoforms in a cell type-, differentiation state-, and activation state-dependent manner.{49671,49676} CD45 also contains two highly conserved cytoplasmic protein tyrosine phosphatase domains that regulate intracellular signaling.{49671} CD45 functions as a negative or positive regulator of antigen receptor signaling in T and B cells through dephosphorylation of Src family kinases in a cell type- and differentiation state-dependent manner.{49676} It also dephosphorylates JAK kinases, inhibiting cytokine and chemokine signaling in leukocytes. Mutations in PTPRC are associated with severe combined immunodeficiency (SCID) in mice and humans.{49672,49673,49674} The number of CD45+ cells is increased in postmortem brain from patients with Alzheimer’s disease.{49675} Increased levels of the CD45 isoform CD45RO have been found on CD19+ lamina propria B cells isolated from patients with Crohn’s disease.{49676} Fluorescently labeled versions of CD45 have commonly been used as a pan leukocyte marker in flow cytometry. Cayman’s CD45 (C-Term) Rabbit Monoclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications.  

     

    Brand:
    Cayman
    SKU:32236 - 100 µl

    Available on backorder

  • CD45 is a type I transmembrane glycoprotein that is encoded by the PTPRC gene in humans and is abundantly expressed on nucleated hematopoietic cells.{49671} It contains an extracellular region that binds to glycoproteins and exists in various isoforms in a cell type-, differentiation state-, and activation state-dependent manner.{49671,49676} CD45 also contains two highly conserved cytoplasmic protein tyrosine phosphatase domains that regulate intracellular signaling.{49671} CD45 functions as a negative or positive regulator of antigen receptor signaling in T and B cells through dephosphorylation of Src family kinases in a cell type- and differentiation state-dependent manner.{49676} It also dephosphorylates JAK kinases, inhibiting cytokine and chemokine signaling in leukocytes. Mutations in PTPRC are associated with severe combined immunodeficiency (SCID) in mice and humans.{49672,49673,49674} The number of CD45+ cells is increased in postmortem brain from patients with Alzheimer’s disease.{49675} Increased levels of the CD45 isoform CD45RO have been found on CD19+ lamina propria B cells isolated from patients with Crohn’s disease.{49676} Fluorescently labeled versions of CD45 have commonly been used as a pan leukocyte marker in flow cytometry. Cayman’s CD45 Monoclonal Antibody (Clone BRA-55) can be used for flow cytometry, Western blot, immunocytochemistry, and immunohistochemistry applications. The antibody recognizes CD45 at 170-220 kDa from human samples.  

     

    Brand:
    Cayman
    SKU:10004597 - 1 ea

    Available on backorder

  • This monoclonal antibody is labeled with fluorescein and intended for direct labeling of human CD5-expressing cells. CD5 is a type I transmembrane glycoprotein (Mr = 67 kDa), expressed on mature T-cells, the majority of thymocytes and B1a-subpopulation of B-cells.{17365} CD5 is a marker of lymphocytes in autoimmune disorders (rheumatoid arthritis, systemic lupus erythematosis), B-cell chronic leukemia, T-cell acute lymphoblastic leukemia, small lymphocytic, and mantle cell non-Hodgkin’s lymphoma. Enough antibody for 100 x 3 µg tests is provided per vial.  

     

    Brand:
    Cayman
    SKU:10232 - 1 ea

    Available on backorder

  • CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24 amino acid transmembrane region and an approximately 150 amino acid lumenal domain.{15683} The CD74 chain is thought to function mainly as a major histocompatibility complex (MHC) class II chaperone, which promotes endoplasmic reticulum (ER) exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and invariant chain expression was believed to be restricted to classical antigen-presenting cells (APC); however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules.{15684} Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes.{15685} CD74 however has also recently been shown to have a role as an accessory-signalling molecule because of its macrophage migration-inhibitory factor (MIF).{15685} The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases.{15686}  

     

    Brand:
    Cayman
    SKU:10011432 - 100 µg

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  • CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24 amino acid transmembrane region and an approximately 150 amino acid lumenal domain.{15683} The CD74 chain is thought to function mainly as a major histocompatibility complex (MHC) class II chaperone, which promotes endoplasmic reticulum (ER) exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and invariant chain expression was believed to be restricted to classical antigen-presenting cells (APC); however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules.{15684} Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes.{15685} CD74 however has also recently been shown to have a role as an accessory-signalling molecule because of its macrophage migration-inhibitory factor (MIF).{15685} The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases.{15686}  

     

    Brand:
    Cayman
    SKU:10011432 - 25 µg

    Available on backorder

  • CD8 is a type I transmembrane glycoprotein that functions as a T cell receptor (TCR) co-receptor.{53531} It exists as an αα homodimer or αβ heterodimer and is composed of an extracellular IgV-like domain that interacts with MHC class I molecules and a cytoplasmic tail that associates with the tyrosine kinase p56Ick and mediates signal transduction to the TCR. It is expressed on the surface of, and used as a marker for, cytotoxic T lymphocytes (CTLs). CD8 is also expressed on natural killer (NK) and dendritic cells and its expression is used to characterize the development stage of thymocytes. CD8 promotes CTL-mediated killing of virally infected cells or cancer cells by binding to antigen-displaying MHC class I molecules and enhancing TCR-mediated intracellular signaling pathways.{53532} Neutralization of circulating CD8+ cells with monoclonal antibodies reduces hepatic virus elimination and decreases splenic CTL activity in a mouse model of lymphocytic choriomeningitis virus (LCMV) infection.{53533} Adoptive transfer of antigen-experienced CD8+ T cells, in combination with IL-2, reduces tumor growth and increases survival in a B16/F10 murine melanoma model.{53534} The number of circulating CD8+ T cells is increased in individuals with HIV-1 infection and is associated with stroke, ischemic heart disease, and non-AIDS-related complications.{53535} Cayman’s CD8 Monoclonal Antibody (Clone RIV11) can be used for flow cytometry (FC), immunocytochemistry (ICC), and immunohistochemistry (IHC) applications.  

     

    Brand:
    Cayman
    SKU:10004715 - 1 ea

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  • CD95 is a type I transmembrane glycoprotein receptor encoded by the FAS gene in humans.{49679} Alternative splicing of FAS generates a soluble form of the protein. CD95 is expressed by activated T and B cells, and thymocytes and has been found in the thymus, liver, heart, and kidney.{49678,49680,49681} CD95 is involved in the induction of apoptosis, as Fas ligand activation of CD95 leads to formation of a death-inducing signaling complex (DISC) comprised of CD95 oligomers, the Fas-associated death domain protein (FADD), procaspase-8, procaspase-10, and c-FLIP.{49682} DISC formation and CD95 internalization is rapid in type I apoptotic cells, where is it associated with plasma membrane lipid rafts, and delayed in type II apoptotic cells, where it is found in both lipid raft- and non-raft regions of the membrane.{49683,11907} CD95 has non-apoptotic activity as well, including activation of the NF-κB signaling pathway and inducing renal tubular epithelial cell migration, among others.{49681} Inhibition or activation of CD95 reduces or promotes cancer cell functions, respectively, in vitro and in vivo in animal models. However, high serum levels of CD95 in patients with various cancers are associated with metastasis, progression, and shorter survival. Mice lacking the gene for CD95 develop spontaneous autoimmunity and have been used as a model of systemic lupus erythematosus (SLE). Mutations in FAS are associated with various cancers and autoimmune lymphoproliferative syndrome (ALPS) type 1a, which is characterized by non-malignant lymphadenopathy and splenomegaly.{49682,49684} Cayman’s CD95 Monoclonal Antibody (Clone IPO-4) can be used for flow cytometry, immunocytochemistry (ICC), and immunohistochemistry (IHC; frozen sections) applications. The antibody recognizes CD95 from human samples.  

     

    Brand:
    Cayman
    SKU:10004604 - 1 ea

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  • Brand:
    Cayman
    SKU:600613 - 50 µl

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  • Brand:
    Cayman
    SKU:601104 - 25 µL

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    Cayman
    SKU:600094 - 5 ea

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    Cayman
    SKU:600302 - 50 ml

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    Cayman
    SKU:600803 - 200 µl

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    Cayman
    SKU:600472 - 100 µl

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    Cayman
    SKU:600175 - 1 ea

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    Cayman
    SKU:10009906 - 1 ea

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    Cayman
    SKU:600172 - 50 ml

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    Cayman
    SKU:10009322 - 1 ea

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    Cayman
    SKU:10009322 - 2 ea

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    Cayman
    SKU:10009322 - 3 ea

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    Cayman
    SKU:10009322 - 4 ea

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    Cayman
    SKU:10009322 - 5 ea

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    Cayman
    SKU:400146 - 1 ea

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  • Brand:
    Cayman
    SKU:600054 - 10 mg

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  • Brand:
    Cayman
    SKU:600054 - 2 mg

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  • Brand:
    Cayman
    SKU:601105 - 50 µL

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    Cayman
    SKU:600371 - 50 µl

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    Cayman
    SKU:600321 - 30 µl

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  • Brand:
    Cayman
    SKU:600092 - 250 µl

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  • Brand:
    Cayman
    SKU:600615 - 2.5 ml

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  • Cell-Based Assay EGCG has been formulated and tested to work with Cayman’s Assay Kits. Detailed instructions for its use are contained in the respective Cayman Assay Kit booklet.  

     

    Brand:
    Cayman
    SKU:10011096 - 1 ea

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  • Brand:
    Cayman
    SKU:10009899 - 1 ea

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  • Brand:
    Cayman
    SKU:600802 - 2 mg

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  • Brand:
    Cayman
    SKU:600362 - 3 mg

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  • Brand:
    Cayman
    SKU:600155 - 1 ea

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  • Brand:
    Cayman
    SKU:600332 - 50 µl

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  • Brand:
    Cayman
    SKU:600053 - 100 µL

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  • Brand:
    Cayman
    SKU:600053 - 500 µL

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  • Brand:
    Cayman
    SKU:600052 - 100 µL

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  • Brand:
    Cayman
    SKU:600052 - 500 µL

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  • Brand:
    Cayman
    SKU:600051 - 100 µl

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  • Brand:
    Cayman
    SKU:10008978 - 1 ea

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  • Brand:
    Cayman
    SKU:10010215 - 1 ea

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  • Brand:
    Cayman
    SKU:600361 - 100 µl

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  • Brand:
    Cayman
    SKU:600621 - 50 µl

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  • Brand:
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    SKU:600622 - 25 µl

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  • Brand:
    Cayman
    SKU:600441 - 500 µl

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  • Brand:
    Cayman
    SKU:600471 - 500 µl

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  • Brand:
    Cayman
    SKU:600612 - 25 ml

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  • Brand:
    Cayman
    SKU:600055 - 1 ea

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  • Brand:
    Cayman
    SKU:600322 - 50 µl

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  • Brand:
    Cayman
    SKU:600096 - 5 ea

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  • Brand:
    Cayman
    SKU:600093 - 5 ea

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  • Brand:
    Cayman
    SKU:600095 - 5 ea

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  • Brand:
    Cayman
    SKU:600744 - 10 ml

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  • Brand:
    Cayman
    SKU:10009869 - 1 ea

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  • Brand:
    Cayman
    SKU:600372 - 50 µl

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  • Brand:
    Cayman
    SKU:10009866 - 1 ea

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  • Brand:
    Cayman
    SKU:600091 - 5 ea

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  • Retinol is an essential micronutrient that plays a critical role in various physiological functions including cell growth and differentiation. Due to the instability and low solubility of retinol in the aqueous medium, it requires specific binding proteins in body fluids and within the cell for transport.{14372} Cytoplasmic carriers of the retinol molecule, the cellular retinol-binding proteins (CRBPs), are monomeric proteins of about 15 kDa. cRBP7 is the most recently indentified cellular retinol carrier found to be expressed mainly in the kidney, heart, and transverse colon in humans.{13310} In addition to the intracellular transport role of cRBR7, further study of this protein is warranted. Cayman · s cRBP7 polyclonal antibody detects both the mature protein at about 15 kDa by western blot in tissues/cells such as heart, HEK 293 cells, COS-7 cells, and RAW 264.7 cells. The antibody can also be used for immunocytochemistry.  

     

    Brand:
    Cayman
    SKU:10010250 - 1 ea

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  • Cayman’s CFSE Cell Division Assay Kit provides an easy-to-use format for labeling and tracing cells through successive cell divisions. Carboxyfluorescein diacetate, succinimidyl ester (CFDA-SE) diffuses into cells, where the acetate groups are cleaved to yield a highly fluorescent derivative (CFSE) that is retained in the cell and can be detected by flow cytometry. Cell division results in sequential halving of fluorescence, and up to eight divisions can be monitored before the fluorescence is decreased to the background fluorescence of unstained cells.{17802,17820} The ease of use and lack of cytotoxicity allows monitoring of cellular division over weeks either in vitro or in vivo. The kit contains sufficient reagents for labeling and analyzing up to 7.5×108 cells by flow cytometry. CFSE-labeled cells can be further stained with any fluorochrome compatible with fluorescein for use in flow cytometry.  

     

    Brand:
    Cayman
    SKU:10009853 - 100 tests

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  • Cyclic GMP-AMP (cGAMP) synthase (cGAS; Item No. 22810) is a nucleotidyltransferase located in the cytosol that acts as a cytosolic DNA sensor to detect foreign DNA from microbial pathogens as part of the innate immune response.{22400,22401} Upon binding to cytosolic DNA, cGAS produces the cyclic dinucleotide second messenger cGAMP, which activates stimulator of interferon genes (STING), leading to activation of the type I interferon (IFN) pathway.{22400,22401,39161} In vitro, fibroblasts, macrophages, and dendritic cells isolated from cGAS knockout (cGAS-/-) mice do not produce type I IFNs following DNA transfection or DNA virus infection.{39163} Similarly, cells containing a frame-shift mutation in the cGAS locus fail to mount an immune response to HIV and other retroviruses.{39164} In vivo, cGAS-/- mice infected with herpes simplex virus 1 (HSV-1) have lower levels of IFN-α and IFN-β, shorter survival times, and higher post-mortem levels of HSV-1 in the brain.{39163} Cayman’s cGAS Monoclonal Antibody (Clone 5G10) recognizes the full length human cGAS protein at ~59 kDa.  

     

    Brand:
    Cayman
    SKU:23853 - 300 µg

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  • Calcitonin gene-related peptide (CGRP) is a 37-amino acid neuropeptide.{41395} It exists in two major forms that share greater than 90% sequence homology, α-CGRP that is produced by alternative RNA splicing of the calcitonin gene CALCI and β-CGRP that is encoded by CALCII in humans. CGRP is primarily expressed in A and Cδ fibers and exhibits perivascular localization but is also expressed in keratinocytes and endothelial progenitor cells.{41395,53682} CGRP binds to a heteromeric complex of calcitonin receptor-like receptor (CRLR) and receptor activity-modifying protein 1 (RAMP1) and has roles in efferent function, nociception, and vasodilation. Levels of CGRP are increased in various pain states, including migraine. It also suppresses TNF-α, increases fibroblast motility and extracellular matrix synthesis, and induces Schwann cell proliferation to facilitate peripheral nerve regeneration following injury.{53683} Cayman’s CGRP Monoclonal Antibody (Clone 4901) can be used for ICC applications.  

     

    Brand:
    Cayman
    SKU:29254 - 100 µl

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  • Chemokine-Like Receptor 1 (CMKLR1) is a G protein-coupled receptor relevant to the cellular chemotaxis of dendritic cells and macrophages.{13210} This receptor is also expressed in brain, liver, lung, and kidney tissues.{13210,17561} Chemerin, or TIG2, has been identified as the natural ligand for this receptor.{17560} Resolvin E1 has also been identified as a ligand for CMKLR1, acting to dampen cellular responses to inflammation.{13210,17558,17559} Chemerin is an 18 kDa protein that plays a role in immunity, inflammation, chemotaxis, and has been identified as an adipokine.{15020,17557,17561} Human CMKLR1 shares 80% sequence homology with its mouse ortholog.{17560} The human receptor exists in two isoforms, differing only by two amino acids due to a premature stop codon. Isoform one has 363 amino acids and an expected molecular weight of 42.3 kDa. Cayman’s CMKLR1 polyclonal antibody recognizes the C-terminal region of the protein and cell permeabilization is recommended prior to whole cell staining.  

     

    Brand:
    Cayman
    SKU:10325 - 1 ea

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  • Sandwich High Sensitivity ELISA kit for Quantitative Detection of chicken GDF5. 96wells/kit, with removable strips.   

     

    Brand:
    Boster Bio
    SKU:EK1504-CH

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  • Sandwich High Sensitivity ELISA kit for Quantitative Detection of chicken Neurotrophin-3. 96wells/kit, with removable strips.   

     

    Brand:
    Boster Bio
    SKU:EK0472-CH

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  • Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated chicken TGF-beta 2. 96wells/kit, with removable strips.   

     

    Brand:
    Boster Bio
    SKU:EK0981-CH

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  • Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated chicken TGF-beta 3. 96wells/kit, with removable strips.   

     

    Brand:
    Boster Bio
    SKU:EK1103-CH

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  • Sandwich High Sensitivity ELISA kit for Quantitative Detection of Chinese hamster BMP-7. 96wells/kit, with removable strips.   

     

    Brand:
    Boster Bio
    SKU:EK1443-HA

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  • Sandwich High Sensitivity ELISA kit for Quantitative Detection of Chinese hamster CXCL14. 96wells/kit, with removable strips.   

     

    Brand:
    Boster Bio
    SKU:EK1285-HA

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  • Sandwich High Sensitivity ELISA kit for Quantitative Detection of Chinese hamster Endothelin. 96wells/kit, with removable strips.   

     

    Brand:
    Boster Bio
    SKU:EK0945-HA

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  • Sandwich High Sensitivity ELISA kit for Quantitative Detection of Chinese hamster GDF5. 96wells/kit, with removable strips.   

     

    Brand:
    Boster Bio
    SKU:EK1504-HA

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  • Sandwich High Sensitivity ELISA kit for Quantitative Detection of Chinese hamster IGF-1. 96wells/kit, with removable strips.   

     

    Brand:
    Boster Bio
    SKU:EK0377-HA

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  • Sandwich High Sensitivity ELISA kit for Quantitative Detection of Chinese hamster IGF-2. 96wells/kit, with removable strips.   

     

    Brand:
    Boster Bio
    SKU:EK0380-HA

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  • Brand:
    Cayman
    SKU:10008052 - 1 ea

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  • Brand:
    Cayman
    SKU:10008052 - 5 ea

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  • Brand:
    Cayman
    SKU:10008054 - 1 ea

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  • Brand:
    Cayman
    SKU:10008057 - 1 ea

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  • Brand:
    Cayman
    SKU:10008057 - 5 ea

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  • Brand:
    Cayman
    SKU:10008055 - 1 ea

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  • Brand:
    Cayman
    SKU:10008055 - 5 ea

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  • Brand:
    Cayman
    SKU:10008056 - 1 ea

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  • Brand:
    Cayman
    SKU:10008056 - 5 ea

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  • Brand:
    Cayman
    SKU:10008053 - 1 ea

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  • Brand:
    Cayman
    SKU:10008053 - 5 ea

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  • The mechanism for the movement of cholesterol from intracellular sites to their ultimate cellular destination is an unresolved question of fundamental importance to cell biology and medicine. Thus, defining mechanisms of intracellular cholesterol transport and identifying the cellular factors involved are therefore of great interest. Cayman’s Cholesterol Cell-Based Detection Assay Kit includes Filipin III, Fixative, and Wash Buffer in a ready to use format. It provides a simple fluorometric method to study mechanisms and biological factors that regulate cholesterol metabolism or movement within cells. A cholesterol trafficking inhibitor, U-18666A, is included as a positive control.  

     

    Brand:
    Cayman
    SKU:10009779 - 2 x 96 wells

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  • Brand:
    Cayman
    SKU:10009868 - 1 ea

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  • Cholesterol, particularly in the form of low density lipoproteins (LDL), is well understood to be associated with increased risk of coronary heart disease. The measurement of cholesterol is one of the most common tests performed in the clinical laboratory setting. However, simple and easy assays for cholesterol in the research lab have not been readily available. Cayman’s Cholesterol Fluorometric Assay provides a simple fluorometric method for the sensitive quantitation of total cholesterol in plasma or serum. In our assay, cholesteryl esters in the sample are first hydrolyzed by cholesterol esterase. All cholesterol, whether previously esterified or existing free in the circulation, is then oxidized by cholesterol oxidase to the corresponding ketone and hydrogen peroxide. ADHP (10-acetyl-3,7-dihydroxyphenoxazine) is utilized as a highly sensitive and stable probe for hydrogen peroxide. Horseradish peroxidase catalyzes the reaction of ADHP with hydrogen peroxide to yield the highly fluorescent product resorufin which can be monitored using excitation wavelengths of 530-540 nm and emission wavelengths of 585-595 nm.  

     

    Brand:
    Cayman
    SKU:10007640 - 480 wells

    Available on backorder

  • Cholesterol, particularly in the form of low density lipoproteins (LDL), is well understood to be associated with increased risk of coronary heart disease. The measurement of cholesterol is one of the most common tests performed in the clinical laboratory setting. However, simple and easy assays for cholesterol in the research lab have not been readily available. Cayman’s Cholesterol Fluorometric Assay provides a simple fluorometric method for the sensitive quantitation of total cholesterol in plasma or serum. In our assay, cholesteryl esters in the sample are first hydrolyzed by cholesterol esterase. All cholesterol, whether previously esterified or existing free in the circulation, is then oxidized by cholesterol oxidase to the corresponding ketone and hydrogen peroxide. ADHP (10-acetyl-3,7-dihydroxyphenoxazine) is utilized as a highly sensitive and stable probe for hydrogen peroxide. Horseradish peroxidase catalyzes the reaction of ADHP with hydrogen peroxide to yield the highly fluorescent product resorufin which can be monitored using excitation wavelengths of 530-540 nm and emission wavelengths of 585-595 nm.  

     

    Brand:
    Cayman
    SKU:10007640 - 96 wells

    Available on backorder

  • Maintaining body cholesterol homeostasis is critical for normal physiological functions and is achieved by a highly regulated balance of de novo synthesis, dietary cholesterol absorption, biliary clearance, and excretion. However, the mechanisms controlling cholesterol absorption are not well understood. A better understanding of the relationship between cholesterol absorption and synthesis may allow for the development of powerful novel strategies for the prevention and treatment of hypercholesterolemia. Cayman’s Cholesterol Uptake Cell-Based Assay Kit provides a convenient tool for studying cholesterol absorption modulators. The kit employs NBD Cholesterol, a fluorescently tagged cholesterol, as a probe for the detection of cholesterol taken up by cultured cells. U-18666A, a cholesterol intracellular trafficking inhibitor, is included as a positive control.  

     

    Brand:
    Cayman
    SKU:600440 - 1 ea

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  • Choline acetyltransferase (ChAT) is an acyltransferase that catalyzes the synthesis of acetylcholine from acetyl coenzyme A (acetyl-CoA; Item No. 16160) and choline.{55140} It is composed of a catalytic domain flanked on both sides by a substrate binding domain.{55140,55145} ChAT is synthesized in the soma of cholinergic neurons in the central and peripheral nervous system and is transported to nerve terminals where it exists in a soluble form or on synaptic vesicles in a membrane-bound form.{55140} It is used as a marker of cholinergic neurons.{55141} Genome-wide deletion of Chat in mice is perinatal lethal.{55142} Intracerebroventricular transplantation of human HB1.F3 neural stem cells overexpressing CHAT reverses learning and memory deficits in a mouse model of Alzheimer’s disease induced by kainic acid (Item No. 78050).{55143} ChAT activity is reduced in a number of neurodegenerative disorders, including Alzheimer’s disease, amyotrophic lateral sclerosis (ALS), and Huntington’s disease.{55140} SNPs in CHAT are associated with Alzheimer’s disease in humans.{55144} Cayman’s Choline Acetyltransferase Polyclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes ChAT at approximately 70 kDa from human, avian, chicken, guinea pig, mouse, non-human primate, and rat samples.  

     

    Brand:
    Cayman
    SKU:29255 - 100 µl

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  • Brand:
    Cayman
    SKU:701041 - 15 ml

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  • Fibrinogen is a hexameric glycoprotein that has roles in coagulation and hemostasis.{53514,53515} It is comprised of two sets of Aα, Bβ, and γ polypeptide chains encoded by FGA, FGB, and FGG, respectively, in humans.{53514} Fibrinogen is synthesized in hepatocytes and secreted into the plasma. Following thrombin-mediated cleavage of N-terminal fibrinopeptides from the Aα and Bβ chains, yielding the α and β chains, respectively, fibrinogen assembles into fibrin protofibrils and then mature fibers, which provide structure and viscoelasticity to blood clots.{53515,53516,53518} Mutations in FGA, FGB, or FGG have been found in patients with afibrinogenemia or hypofibrinogenemia.{53514} Elevated plasma fibrinogen levels are associated with an increased risk of cardiovascular disease.{53517} Fibrinogen can be citrullinated by protein arginine deiminase 2 (PAD2) and PAD4.{31655} Immune complexes containing citrullinated fibrinogen have been found in patients with anti-citrullinated protein antibody-positive rheumatoid arthritis.{21643} Cayman’s Citrullinated Fibrinogen Monoclonal Antibody (Clone 10E9.3) can be used for ELISA and Western blot (WB) applications. The antibody recognizes citrullinated fibrinogen at 56 kDa from human samples.  

     

    Brand:
    Cayman
    SKU:17088 - 100 µg

    Available on backorder

  • Citrullinated glial fibrillary acidic protein (citGFAP) is a citrullinated form of GFAP (Item No. 27353), a class III intermediate filament (IF) protein that is expressed in mature astrocytes and contributes to cytoskeletal structure and strength.{53009} GFAP can be citrullinated in a calcium-dependent manner on the arginine residue at position 270 (R270) and at R416 by protein arginine deiminase 1 (PAD1; Item No. 10784) and PAD2 (Item No. 10785).{53021} citGFAP levels are increased by the calcium ionophore ionomycin (Item No. 10004974) in an in vitro model of traumatic brain injury (TBI) using NHA CC-2565 human astrocytes and are induced by controlled cortical impact in the cerebral cortex in a rat model of TBI.{53020} It has been found in the hippocampus in a rat model of Alzheimer’s disease induced by amyloid-β (25-35), as well as in the retina in a mouse model of alkali-induced eye injury.{45561,45562} citGFAP has also been found in postmortem hippocampus from patients with Alzheimer’s disease, and citGFAP levels are increased in postmortem-derived brain lesions from patients with multiple sclerosis.{53021,35771} Cayman’s Citrullinated GFAP (R416) Monoclonal Antibody (Clone 4B8) can be used for ELISA, Immunohistochemistry, and Western blot applications. The antibody recognizes recombinant GFAP and citGFAP at 37 kDa.  

     

    Brand:
    Cayman
    SKU:27795 - 300 µg

    Available on backorder

  • Glucose-regulated protein 78 kDa (GRP78) is a molecular chaperone that is ubiquitously expressed in the endoplasmic reticulum of mammalian cells.{36130,36131,36132} GRP78 can be citrullinated at its 27 arginine residues by protein deiminases (PADs).{37332} The accumulation of citrullinated proteins in vivo leads to the production of anti-citrullinated protein antibodies (ACPAs) which perpetuate the inflammatory process.{32067} In vitro, ACPAs bind to citrullinated GRP78 expressed on the cell surface of peripheral blood mononuclear cells PMBCs and U937 cells leading to the production of TNF-α.{37330},{37331} In a mouse model of collagen-induced arthritis (CIA), anti-citrullinated GRP78 antibodies are found in the serum.{37332} Pre-immunization with citrullinated GRP78 prior to CIA induction shortens the time to joint inflammation and increases arthritis scores compared with non-citrullinated GRP78-immunized and non-immunized control mice. In autoimmune diseases such as rheumatoid arthritis, patient-derived serum contains higher levels of anti-citrullinated GRP78 antibodies than serum derived from patients with systemic lupus erythematosus and healthy controls.{37332} The predicted size of GRP78 (R368) is approximately 78 kDa and Cayman’s Citrullinated GRP78 (R368) Polyclonal Antibody detects the citrullinated GRP78 protein but not native GRP78 by Western blot. Cayman’s Citrullinated GRP78 (R368) Polyclonal Antibody detects citrullinated GRP78 and does not detect unmodified GRP78.  

     

    Brand:
    Cayman
    SKU:24287 - 1 ea

    Available on backorder

  • Histone H3 is citrullinated by peptidylarginine deiminase 4 (PAD4) at residues R2, R8, and R17 during the processes of epigenetic chromatin remodeling and neutrophil extracellular trap (NET) formation. In vitro, citrullinated H3 (CitH3) is released from neutrophils that have been stimulated with calcium ionophore during the process of NETosis, and can be freed from NETs by treatment with nuclease. The measurement of CitH3, either in cell lysates or released during NETosis can be used as a functional readout of pharmacological PAD inhibition.  

     

    Brand:
    Cayman
    SKU:501620 - 96 wells

    Available on backorder

  • Heat shock protein 70s (Hsp70s) are abundant and stress-inducible 70 kDa molecular chaperone proteins encoded by a highly conserved, multigene family.{15480} They are monomeric proteins that can be divided into two functional domains: an N-terminal ATPase domain and a substrate binding domain that contains a highly conserved EEVD motif at its C-terminus. Hsp70s are found in the cytosol, nuclei, endoplasmic reticulum, mitochondria, and chloroplasts of eukaryotes, as well as in bacteria. They function as molecular chaperones that assist in a wide range of cellular processes, including refolding of aggregated or misfolded proteins, co- and post-translational folding and assembly of nascent peptides, membrane translocation of secretory and organellar proteins, controlling activity of regulatory nuclear receptors, kinases and transcription factors, as well as cooperativity with the Hsp90 chaperone system in eukaryotes.{36132} The Hsp70 chaperone cycle is ATP-dependent and initiated by transient interaction of the Hsp70 substrate binding domain with hydrophobic regions within a peptide or protein. It consists of an alteration between the low-affinity ATP-bound state with fast rates of substrate exchange and the high-affinity ADP bound state with slow rates of substrate exchange. Hsp70s are subject to a variety of post-translational modifications and their expression is upregulated under conditions of cellular stress and in a variety of disease states. Specifically, citrullinated Hsp70 peptides have been found in the synovial fluid of patients with rheumatoid arthritis.{31556} Cayman’s Citrullinated Hsp70 (R155) Polyclonal Antibody can be used for Western blot and ELISA applications.  

     

    Brand:
    Cayman
    SKU:24497 - 1 ea

    Available on backorder

  • Heat shock protein 70s (Hsp70s) are abundant and stress-inducible 70 kDa molecular chaperone proteins encoded by a highly conserved, multigene family.{15480} They are monomeric proteins that can be divided into two functional domains: an N-terminal ATPase domain and a substrate binding domain that contains a highly conserved EEVD motif at its C-terminus. Hsp70s are found in the cytosol, nuclei, endoplasmic reticulum, mitochondria, and chloroplasts of eukaryotes, as well as in bacteria. They function as molecular chaperones that assist in a wide range of cellular processes, including refolding of aggregated or misfolded proteins, co- and post-translational folding and assembly of nascent peptides, membrane translocation of secretory and organellar proteins, controlling activity of regulatory nuclear receptors, kinases and transcription factors, as well as acting cooperatively with the Hsp90 chaperone system in eukaryotes.{36132} The Hsp70 chaperone cycle is ATP-dependent and initiated by transient interaction of the Hsp70 substrate binding domain with hydrophobic regions within a peptide or protein. It consists of an alteration between the low-affinity ATP-bound state with fast rates of substrate exchange and the high-affinity ADP bound state with slow rates of substrate exchange. Hsp70s are subject to a variety of post-translational modifications and their expression is upregulated under conditions of cellular stress and in a variety of disease states. Specifically, citrullinated Hsp70 peptides have been found in the synovial fluid of patients with rheumatoid arthritis.{31556} Cayman’s Citrullinated Hsp70 (R357) Polyclonal Antibody can be used for Western blot and ELISA applications.  

     

    Brand:
    Cayman
    SKU:23916 - 1 ea

    Available on backorder

  • Citrullinated myelin basic protein (citMBP) is a citrullinated form of MBP, a protein that is integral to myelin stability in the CNS.{42873} MBP contains 19 arginine residues that can be citrullinated by peptidylarginine deiminases (PADs), decreasing the positive charge of MBP.{42873,42874} The loss of positive charge disrupts MBP-lipid interactions, leading to myelin destabilization and loss and increases its susceptibility for degradation. MBP is degraded by the myelin-associated protease cathepsin D, which leads to exposure and release of immunodominant epitopes.{42873,42875} The percentage of MBP that is citrullinated is increased in the postmortem brain from patients with multiple sclerosis (MS), with an even greater percentage citrullinated in Marburg disease, a more severe form of MS.{42873} citMBP isolated from patients with multiple sclerosis (MS) is degraded by cathepsin D at a higher rate than MBP isolated from healthy controls.{42873,42875} Cayman’s Citrullinated Myelin Basic Protein Polyclonal Antibody can be used for Western blot and ELISA applications. The antibody recognizes citMBP at 21 kDa from human samples, and was generated using a peptide immunogen containing citrullines at R122 and R130 (human myelin basic protein sequence, isoform 5).  

     

    Brand:
    Cayman
    SKU:26742 - 500 µl

    Available on backorder

  • Vimentin is a cytoskeleton intermediate filament protein present in cells of mesenchymal origin, including leukocytes, endothelial cells, and smooth muscle cells. It is subject to citrullination by peptidyl arginine deiminase 2 (PAD2; Item No. 10785) under high calcium concentrations, which can occur during macrophage apoptosis.{31884} Citrullinated vimentin has been shown to have a role in the production of anti-citrullinated protein antibodies (ACPAs).{31884,31883,55252} ACPAs against citrullinated proteins, such as vimentin, are considered to be highly specific markers for rheumatoid arthritis and other autoimmune diseases.{31883,55252} Cayman’s Citrullinated Vimentin Monoclonal Antibody (Clone 12G11) can be used for Western blot and ELISA applications. The antibody recognizes citrullinated vimentin at ~54 kDa from human samples.  

     

    Brand:
    Cayman
    SKU:22054 - 100 µg

    Available on backorder

  • α-Enolase, also known as enolase 1, is a glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate.{42086} It is ubiquitously expressed in human tissues, including liver, spleen, kidney, and brain. α-Enolase is an autoantigen in asthma and Hashimoto’s encephalopathy, and has been found in the serum of pediatric patients with juvenile idiopathic arthritis.{42090,42091,42092,36062} α-Enolase can be citrullinated by peptidyl arginine deiminases (PADs) and citrullinated α-enolase has been found in the synovial fluid of rheumatoid arthritis patients.{32062} Cayman’s Citrullinated α-Enolase Polyclonal Antibody can be used for Western blot and ELISA applications. It detects citrullinated α-enolase at ~50 kDa in human samples.  

     

    Brand:
    Cayman
    SKU:25947 - 500 µl

    Available on backorder

  • Cofactor of BRCA1 (COBRA1), also known as negative elongation factor B (NELFB), is a 580-amino acid nuclear protein encoded by NELFB in humans.{46946,46943} It induces chromosome decondensation by targeting lac operator-containing chromosome regions and is a component of NELF, a four-subunit complex that suppresses RNA polymerase II elongation in vitro. COBRA1 protein levels are enriched in mammary gland luminal epithelium, and it has a role in modulating estrogen-dependent and -independent expression of oncogenes in various breast cancer cell lines.{46943} Ectopic expression or knockdown of NELFB suppresses or accelerates breast cancer cell growth, respectively, as well as enhances androgen-mediated transcription.{46943,46944} COBRA1 also interacts with the activated protein-1 (AP-1) family members c-Jun and c-Fos, and inhibits AP-1-mediated transcription in vitro.{46945} Cayman’s COBRA1 Monoclonal Antibody can be used for Western blot (WB) applications. The antibody recognizes COBRA1 at approximately 62 kDa from human, mouse, and rat samples.  

     

    Brand:
    Cayman
    SKU:29256 - 100 µl

    Available on backorder

  • Connexin43 (Cx43) is a membrane protein and member of the connexin family that is encoded by GJA1 in humans.{55146,55147} It is widely expressed across various tissues, including heart, brain, and retina, and exhibits plasma membrane localization. Cx43 is a monomeric component of connexons, homo- or heterohexamers that form hydrophilic pores and facilitate the exchange of ions and secondary messengers between the cytoplasm of connected cells at gap junctions. It is essential to cardiomyocyte function and cardiac development, osteoblast and osteocyte survival, and spermatogenesis.{55146} Cx43 is also expressed in various tumor cells, including liver, prostate, and breast, and exhibits tumor-specific pro- or anti-metastatic effects.{55146,55148} The C-terminus of Cx43 is subject to various post-translational modifications, including phosphorylation. Phosphorylation of Cx43 at position 386 (phospho-Ser368) is mediated by protein kinase C (PKC) and p38 MAPK.{55150,55151} PKC-mediated phosphorylation of Cx43 is increased in a mouse model of acute respiratory distress syndrome (ARDS).{55150} IL-1β increases p38 MAPK-mediated phosphorylation of Cx43 at Ser368 in a perfused rat heart model of myocarditis.{55151} Cayman’s Connexin43 (Phospho-Ser368) Polyclonal Antibody can be used for Western blot (WB) applications. The antibody recognizes connexin43 (phospho-Ser368) at approximately 43 kDa from mouse and rat samples.  

     

    Brand:
    Cayman
    SKU:29258 - 100 µl

    Available on backorder

  • Connexin43 (Cx43) is a membrane protein and member of the connexin family that is encoded by GJA1 in humans.{55146,55147} It is widely expressed across various tissues, including the heart, brain, and retina, and exhibits plasma membrane localization. Cx43 is a monomeric component of connexons, homo- or heterohexamers that form hydrophilic pores and facilitate the exchange of ions and secondary messengers between the cytoplasm of connected cells at gap junctions. It is essential to cardiomyocyte function and cardiac development, osteoblast and osteocyte survival, and spermatogenesis.{55146} Cx43 is also expressed in various tumor cells, including in the liver, prostate, and breast, and exhibits tumor-specific pro- or anti-metastatic effects.{55146,55148} Inhibition of Gja1 with antisense oligonucleotides promotes wound healing in murine skin, skeletal muscle, cardiac tissue, and vascular endothelium, however, global heterozygous knockdown of Gja1 increases infarct size in a mouse model of ischemia and reperfusion injury.{55146,55147} Levels of Cx43 are decreased in postmortem retinal samples from patients with diabetes compared with control individuals.{55149} Cayman’s Connexin43 Polyclonal Antibody can be used for Western blot (WB) applications. The antibody recognizes connexin43 at approximately 43 kDa from rat samples.  

     

    Brand:
    Cayman
    SKU:29257 - 100 µl

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  • Brand:
    Cayman
    SKU:401322 - 100 dtn

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  • Brand:
    Cayman
    SKU:401322 - 500 dtn

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  • Corticosterone is a glucocorticoid produced by the adrenal cortex in response to ACTH (corticotropic hormone) and is the precursor to aldosterone. The production of glucorcorticoids is increased by stress; therefore, corticosterone can be used as a biomarker of stress.{11468} Biologists often measure corticosterone in fecal material of the species they are studying.{11468,11469} This non-invasive sample collection procedure has the advantage of not itself causing stress and thereby increasing corticosterone levels, a common problem when samples for measurement of corticosterone are collected by more invasive means.{11468} Plasma corticosterone levels have a circadian variation and corticosterone may be important in the regulation of the sleep-wake cycle.{11469,11480} Cayman’s Corticosterone ELISA Kit is a competitive assay that can be used for quantification of corticosterone in serum and rodent feces.  

     

    Brand:
    Cayman
    SKU:501320 - 480 solid wells

    Available on backorder

  • Corticosterone is a glucocorticoid produced by the adrenal cortex in response to ACTH (corticotropic hormone) and is the precursor to aldosterone. The production of glucorcorticoids is increased by stress; therefore, corticosterone can be used as a biomarker of stress.{11468} Biologists often measure corticosterone in fecal material of the species they are studying.{11468,11469} This non-invasive sample collection procedure has the advantage of not itself causing stress and thereby increasing corticosterone levels, a common problem when samples for measurement of corticosterone are collected by more invasive means.{11468} Plasma corticosterone levels have a circadian variation and corticosterone may be important in the regulation of the sleep-wake cycle.{11469,11480} Cayman’s Corticosterone ELISA Kit is a competitive assay that can be used for quantification of corticosterone in serum and rodent feces.  

     

    Brand:
    Cayman
    SKU:501320 - 480 strip wells

    Available on backorder

  • Corticosterone is a glucocorticoid produced by the adrenal cortex in response to ACTH (corticotropic hormone) and is the precursor to aldosterone. The production of glucorcorticoids is increased by stress; therefore, corticosterone can be used as a biomarker of stress.{11468} Biologists often measure corticosterone in fecal material of the species they are studying.{11468,11469} This non-invasive sample collection procedure has the advantage of not itself causing stress and thereby increasing corticosterone levels, a common problem when samples for measurement of corticosterone are collected by more invasive means.{11468} Plasma corticosterone levels have a circadian variation and corticosterone may be important in the regulation of the sleep-wake cycle.{11469,11480} Cayman’s Corticosterone ELISA Kit is a competitive assay that can be used for quantification of corticosterone in serum and rodent feces.  

     

    Brand:
    Cayman
    SKU:501320 - 96 solid wells

    Available on backorder

  • Corticosterone is a glucocorticoid produced by the adrenal cortex in response to ACTH (corticotropic hormone) and is the precursor to aldosterone. The production of glucorcorticoids is increased by stress; therefore, corticosterone can be used as a biomarker of stress.{11468} Biologists often measure corticosterone in fecal material of the species they are studying.{11468,11469} This non-invasive sample collection procedure has the advantage of not itself causing stress and thereby increasing corticosterone levels, a common problem when samples for measurement of corticosterone are collected by more invasive means.{11468} Plasma corticosterone levels have a circadian variation and corticosterone may be important in the regulation of the sleep-wake cycle.{11469,11480} Cayman’s Corticosterone ELISA Kit is a competitive assay that can be used for quantification of corticosterone in serum and rodent feces.  

     

    Brand:
    Cayman
    SKU:501320 - 96 strip wells

    Available on backorder

  • Brand:
    Cayman
    SKU:400657 - 1 ea

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  • Cortisol is a glucocorticoid produced by the adrenal cortex in response to adrenocorticotropic hormone (ACTH). It is secreted with a circadian periodicity, and peaks just prior to waking in the morning. Cortisol is often elevated in major depressive disorder, certain forms of hypertension, stress, AIDS, and in the visceral fat of obese individuals. Cortisol can be measured in many matrices including blood, urine, and saliva. In serum, approximately 90-95% of cortisol is bound to proteins. Urinary cortisol is not bound to proteins, but its levels are dependent on glomerular and tubular function. In saliva, approximately 67% of cortisol is unbound. There is generally good correlation between cortisol measurements in saliva and serum. Cayman’s Cortisol ELISA Kit is a competitive assay that can be used for quantification of cortisol in urine, plasma, and other sample matrices. The assay has a range from 6.6-4,000 pg/ml and a sensitivity (80% B/B0) of approximately 35 pg/ml.NOTE: Due to an approximate 15% cross-reactivity with dexamethasone, this kit may not be suitable for use with samples that contain high concentrations of dexamethasone, such as those collected during a dexamethasone suppression test.  

     

    Brand:
    Cayman
    SKU:500360 - 480 solid wells

    Available on backorder

  • Cortisol is a glucocorticoid produced by the adrenal cortex in response to adrenocorticotropic hormone (ACTH). It is secreted with a circadian periodicity, and peaks just prior to waking in the morning. Cortisol is often elevated in major depressive disorder, certain forms of hypertension, stress, AIDS, and in the visceral fat of obese individuals. Cortisol can be measured in many matrices including blood, urine, and saliva. In serum, approximately 90-95% of cortisol is bound to proteins. Urinary cortisol is not bound to proteins, but its levels are dependent on glomerular and tubular function. In saliva, approximately 67% of cortisol is unbound. There is generally good correlation between cortisol measurements in saliva and serum. Cayman’s Cortisol ELISA Kit is a competitive assay that can be used for quantification of cortisol in urine, plasma, and other sample matrices. The assay has a range from 6.6-4,000 pg/ml and a sensitivity (80% B/B0) of approximately 35 pg/ml.NOTE: Due to an approximate 15% cross-reactivity with dexamethasone, this kit may not be suitable for use with samples that contain high concentrations of dexamethasone, such as those collected during a dexamethasone suppression test.  

     

    Brand:
    Cayman
    SKU:500360 - 480 strip wells

    Available on backorder

  • Cortisol is a glucocorticoid produced by the adrenal cortex in response to adrenocorticotropic hormone (ACTH). It is secreted with a circadian periodicity, and peaks just prior to waking in the morning. Cortisol is often elevated in major depressive disorder, certain forms of hypertension, stress, AIDS, and in the visceral fat of obese individuals. Cortisol can be measured in many matrices including blood, urine, and saliva. In serum, approximately 90-95% of cortisol is bound to proteins. Urinary cortisol is not bound to proteins, but its levels are dependent on glomerular and tubular function. In saliva, approximately 67% of cortisol is unbound. There is generally good correlation between cortisol measurements in saliva and serum. Cayman’s Cortisol ELISA Kit is a competitive assay that can be used for quantification of cortisol in urine, plasma, and other sample matrices. The assay has a range from 6.6-4,000 pg/ml and a sensitivity (80% B/B0) of approximately 35 pg/ml.NOTE: Due to an approximate 15% cross-reactivity with dexamethasone, this kit may not be suitable for use with samples that contain high concentrations of dexamethasone, such as those collected during a dexamethasone suppression test.  

     

    Brand:
    Cayman
    SKU:500360 - 96 solid wells

    Available on backorder

  • Cortisol is a glucocorticoid produced by the adrenal cortex in response to adrenocorticotropic hormone (ACTH). It is secreted with a circadian periodicity, and peaks just prior to waking in the morning. Cortisol is often elevated in major depressive disorder, certain forms of hypertension, stress, AIDS, and in the visceral fat of obese individuals. Cortisol can be measured in many matrices including blood, urine, and saliva. In serum, approximately 90-95% of cortisol is bound to proteins. Urinary cortisol is not bound to proteins, but its levels are dependent on glomerular and tubular function. In saliva, approximately 67% of cortisol is unbound. There is generally good correlation between cortisol measurements in saliva and serum. Cayman’s Cortisol ELISA Kit is a competitive assay that can be used for quantification of cortisol in urine, plasma, and other sample matrices. The assay has a range from 6.6-4,000 pg/ml and a sensitivity (80% B/B0) of approximately 35 pg/ml.NOTE: Due to an approximate 15% cross-reactivity with dexamethasone, this kit may not be suitable for use with samples that contain high concentrations of dexamethasone, such as those collected during a dexamethasone suppression test.  

     

    Brand:
    Cayman
    SKU:500360 - 96 strip wells

    Available on backorder

  • Brand:
    Cayman
    SKU:400364 - 1 ea

    Available on backorder

  • Cayman’s Cortisol Express ELISA is a competitive assay that permits the rapid measurement of cortisol from biological samples, requiring only a two hour incubation and one hour development times. This ELISA offers the convenience of a fast assay while maintaining sensitivity.NOTE: Due to an approximate 15% cross-reactivity with dexamethasone, this kit may not be suitable for use with samples that contain high concentrations of dexamethasone, such as those collected during a dexamethasone suppression test.  

     

    Brand:
    Cayman
    SKU:500370 - 480 solid wells

    Available on backorder

  • Cayman’s Cortisol Express ELISA is a competitive assay that permits the rapid measurement of cortisol from biological samples, requiring only a two hour incubation and one hour development times. This ELISA offers the convenience of a fast assay while maintaining sensitivity.NOTE: Due to an approximate 15% cross-reactivity with dexamethasone, this kit may not be suitable for use with samples that contain high concentrations of dexamethasone, such as those collected during a dexamethasone suppression test.  

     

    Brand:
    Cayman
    SKU:500370 - 480 strip wells

    Available on backorder

  • Cayman’s Cortisol Express ELISA is a competitive assay that permits the rapid measurement of cortisol from biological samples, requiring only a two hour incubation and one hour development times. This ELISA offers the convenience of a fast assay while maintaining sensitivity.NOTE: Due to an approximate 15% cross-reactivity with dexamethasone, this kit may not be suitable for use with samples that contain high concentrations of dexamethasone, such as those collected during a dexamethasone suppression test.  

     

    Brand:
    Cayman
    SKU:500370 - 96 solid wells

    Available on backorder

  • Cayman’s Cortisol Express ELISA is a competitive assay that permits the rapid measurement of cortisol from biological samples, requiring only a two hour incubation and one hour development times. This ELISA offers the convenience of a fast assay while maintaining sensitivity.NOTE: Due to an approximate 15% cross-reactivity with dexamethasone, this kit may not be suitable for use with samples that contain high concentrations of dexamethasone, such as those collected during a dexamethasone suppression test.  

     

    Brand:
    Cayman
    SKU:500370 - 96 strip wells

    Available on backorder

  • Brand:
    Cayman
    SKU:400374 - 1 ea

    Available on backorder

  • The COX (human) Inhibitor Screening Assay Kit directly measures prostaglandin F2α (PGF2α) by SnCl2 reduction of COX-derived PGH2 produced in the COX reaction. The prostanoid product is quantified via enzyme-linked immunosorbent assay (ELISA) using a broadly specific antiserum that binds to all the major prostaglandin compounds. This assay includes human recombinant COX-1 and COX-2, allowing the user to screen specific inhibitors. It is an excellent tool for general inhibitor screening or for eliminating false positive leads generated by less specific methods.  

     

    Brand:
    Cayman
    SKU:701230 - 96 wells

    Available on backorder

  • The COX Colorimetric Inhibitor Screening Assay measures the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized N,N,N’,N’-tetramethyl-p-phenylenediamine (TMPD) at 590 nm.{8581} Inhibition of COX activity, measured by TMPD oxidation, by a variety of selective and nonselective inhibitors, shows changed potencies similar to those observed with other in vitro methods. The COX Colorimetric Inhibitor Screening assay includes both ovine COX-1 and COX-2 enzymes in order to screen isozyme-specific inhibitors. The Cayman COX Colorimetric Assay is a time saving tool for screening vast numbers of inhibitors.  

     

    Brand:
    Cayman
    SKU:760111 - 2 x 96 wells

    Available on backorder

  • The COX (ovine) Inhibitor Screening Assay directly measures PGF2α produced in the cyclooxygenase reaction. The prostanoid product is quantified via enzyme immunosorbent assay (ELISA) using a broadly specific antibody that binds to all the major prostaglandin compounds. Thus, this Cayman COX assay is more accurate and reliable than an assay based on peroxidase inhibition. The Cayman COX (ovine) Inhibitor Screening Assay includes both ovine COX-1 and COX-2 enzymes in order to screen isozyme-specific inhibitors. This assay is an excellent tool which can be used for general inhibitor screening, or to eliminate false positive leads generated by less specific methods.  

     

    Brand:
    Cayman
    SKU:560101 - 96 wells

    Available on backorder

  • Cyclooxygenase (COX, also called Prostaglandin H Synthase or PGHS) enzymes contain both cyclooxygenase and peroxidase activities. COX catalyzes the first step in the biosynthesis of prostaglandins (PGs), thromboxanes, and prostacyclins; the conversion of arachidonic acid to PGH2. It is now well established that there are two distinct isoforms of COX. Cyclooxygenase-1 (COX-1) is constitutively expressed in variety of cell types and is involved in normal cellular homeostasis. A variety of mitogenic stimuli such as phorbol esters, lipopolysaccharides, and cytokines lead to the induced expression of a second isoform of COX, cyclooxygenase-2 (COX-2). COX-2 is responsible for the biosynthesis of PGs under acute inflammatory conditions.{4} This inducible COX-2 is believed to be the target enzyme for the anti-inflammatory activity of nonsteroidal anti-inflammatory drugs. The COX (ovine/human) Inhibitor Screening Assay directly measures PGF2α produced by SnCl2 reduction of COX-derived PGH2. The prostanoid product is quantified via enzyme immunosorbent assay (ELISA) using a broadly specific antibody that binds to all the major prostaglandin compounds. Thus, the Cayman COX assay is more accurate and reliable than an assay based on peroxidase inhibition. The Cayman COX Inhibitor Screening Assay includes both ovine COX-1 and human recombinant COX-2 enzymes in order to screen isozyme-specific inhibitors. This assay is an excellent tool which can be used for general inhibitor screening, or to eliminate false positive leads generated by less specific methods.  

     

    Brand:
    Cayman
    SKU:560131 - 96 wells

    Available on backorder

  • The COX Activity Assay utilizes the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized N,N,N’,N’-tetramethyl-p-phenylenediamine (TMPD) at 590 nm.{8581} The assay can be used to measure COX activity (COX-1 and COX-2) in cell lysates, tissue homogenates, and purified enzyme preparations. The assay includes COX-1 and COX-2 specific inhibitors in order to distinguish between the two isozymes.  

     

    Brand:
    Cayman
    SKU:760151 - 96 wells

    Available on backorder

  • The COX Colorimetric Inhibitor Screening Assay measures the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized N,N,N’,N’-tetramethyl-p-phenylenediamine (TMPD) at 590 nm.{8581} Inhibition of COX activity, measured by TMPD oxidation, by a variety of selective and nonselective inhibitors, shows changed potencies similar to those observed with other in vitro methods. The COX Colorimetric Inhibitor Screening assay includes both ovine COX-1 and human recombinant COX-2 enzymes in order to screen isozyme-specific inhibitors. The Cayman COX Colorimetric Assay is a time saving tool for screening vast numbers of inhibitors.  

     

    Brand:
    Cayman
    SKU:701050 - 2 x 96 wells

    Available on backorder

  • Cyclooxygenase (COX, also called Prostaglandin H Synthase or PGHS) is a bifunctional enzyme exhibiting both COX and peroxidase activities. The COX component converts arachidonic acid to a hydroperoxy endoperoxide (PGG2), and the peroxidase component reduces the endoperoxide to the corresponding alcohol (PGH2), the precursor of PGs, thromboxanes, and prostacyclins.{500,501} It is now well established that there are two distinct isoforms of COX, namely COX-1 and COX-2. Cayman’s COX Fluorescent Activity Assay provides a convenient fluorescence-based method for detecting COX-1 or COX-2 activity in both crude (cell lysates/tissue homogenates) and purified enzyme preparations. The assay utilizes the peroxidase component of COXs. In this assay, the reaction between PGG2 and ADHP (10-acetyl-3,7-dihydroxyphenoxazine) produces the highly fluorescent compound resorufin that can be analyzed using an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm. The kit includes isozyme-specific inhibitors for distinguishing COX-2 activity from COX-1 activity.  

     

    Brand:
    Cayman
    SKU:700200 - 2 x 96 wells

    Available on backorder