Chicken TGF-Beta 2 PicoKine™ ELISA Kit

Brand:
Boster Bio
Storage:

Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated chicken TGF-beta 2. 96wells/kit, with removable strips.   

 

Available on backorder

SKU: EK0981-CH Category:

Description

Boster’s Chicken TGF-beta 2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGF-beta 2 has been precoated onto 96-well plates. Standards(NSO, A303-S414) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF-beta 2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Chicken TGF-beta 2 amount of sample captured in plate.


Synonyms: Transforming growth factor beta-2;TGF-beta-2;BSC-1 cell growth inhibitor;Cetermin;Glioblastoma-derived T-cell suppressor factor;G-TSF;Polyergin;Latency-associated peptide;LAP;TGFB2;

Species: Chicken TGFB2

Assay: 31.2pg/ml-2000pg/ml

Sensitivy: <10pg/ml Specifity:

Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

Antibodies: monoclonal antibody from mouse|polyclonal antibody from goat

Anticoagulant: heparin, EDTA or citrate

Immunogen: Expression system for standard: NSO; Immunogen sequence: A303-S414

Gene name: Transforming growth factor beta-2 / Transforming growth factor beta-2

Protein name: Transforming growth factor beta-2

Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)


Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 16 16 16 24 24 24
Mean(pg/ml) 221 564 1145 236 576 1095
Standard deviation 13.7 31.6 46.9 15.8 33.41 70.1
CV(%) 6.2 5.6 4.1 6.7 5.8 6.4

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%)
Sample 1 49 57 47 51 51 3.74 7.3%
Sample 2 188 208 181 193 192 9.91 5.1%
Sample 3 820 783 823 759 796 26.65 3.3%

*number of samples for each test n=16.


10ng/tube