Description
Boster’s rat TNFSF12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFSF12 has been precoated onto 96-well plates. Standards(E.coli, S94-H249) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat TNFSF12 amount of sample captured in plate.
Synonyms: APO 3 ligand|APO 3L|APO3 ligand|APO3/DR3 ligand|APO3L|CD255|DR3LG|MGC129581|MGC20669|TNF related weak inducer of apoptosis|TNF-related weak inducer of apoptosis|TNF12_HUMAN|TNFRS12a|TNFSF 12|TNFSF12|TNFSF12 protein|Tumor necrosis factor (ligand) superfamily member 12|Tumor necrosis factor ligand superfamily member 12|Tumor necrosis factor superfamily member 12|TWEAK|UNQ181/PRO207
Species: rat TNFSF12
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant rat TNFSF12
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, S94-H249
Gene name: Tumor necrosis factor ligand superfamily member 12 / tumor necrosis factor (ligand) superfamily, member 12
Protein name: Tumor necrosis factor ligand superfamily member 12
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|31|228|507|28|218|506
Standard deviation|1.7|9.8|30.42|1.96|9.59|37.44
CV(%)|5.5%|4.3%|6%|7%|4.4%|7.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 31 | 31 | 31 | 28 | 30 | 1.29 | 4.3% |
| Sample 2 | 228 | 219 | 215 | 203 | 216 | 8.98 | 4.1% |
| Sample 3 | 507 | 509 | 522 | 445 | 495 | 29.86 | 6% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1177-CAP|96-well plate precoated with anti- rat TNFSF12 antibody|1
EK1177-ST|Lyophilized recombinant rat TNFSF12 standard|10ng/tube×2
EK1177-DA|Biotinylated anti- rat TNFSF12 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
