Rat BDNF PicoKine™ One Step ELISA Kit

Brand:
Boster Bio
Storage:

For the quantitation of Rat Bdnf concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA, citrate).   

 

Available on backorder

SKU: FEK0308 Category:

Description

The Boster One Step Picokine™ Rat Bdnf Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Bdnf with a 96-well strip plate that is pre-coated with antibody specific for Bdnf. The detection antibody is a HRP conjugated antibody specific for Bdnf. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat Bdnf with immunogen: Expression system for standard: sf21; Immunogen sequence: H129-R247. The kit is analytically validated with ready to use reagents.
To measure Rat Bdnf, add standards and samples to the wells, then add HRP conjugated detection antibody. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Rat Bdnf in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Rat Bdnf in the sample. For more information on assay principle, protocols, and troubleshooting tips, see Boster’s ELISA Resource Center at https://www.bosterbio.com/elisa-technical-resource-center.


Synonyms: Bdnf; Brain-derived neurotrophic factor; BDNF [Cleaved into: BDNF precursor form|ProBDNF]

Species: Rat Bdnf

Assay: 31.2pg/ml-2000pg/ml

Sensitivy: <15pg/ml Specifity: Natural and recombinant Rat Bdnf

Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

Antibodies: monoclonal antibody from mouse|polyclonal antibody from goat

Anticoagulant: heparin, EDTA, citrate

Immunogen: Expression system for standard: sf21; Immunogen sequence: H129-R247

Gene name: Brain-derived neurotrophic factor / brain-derived neurotrophic factor

Protein name: Brain-derived neurotrophic factor

Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.(Shipped with wet ice.)


|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|95|302|947|88|289|1024
Standard deviation|4.94|16.91|72.91|4.75|21.67|86.01
CV(%)|5.2%|5.6%|7.7%|5.4%|7.5%|8.4%


Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%)
Sample 1 95 98 94 105 98 6.17 6.3%
Sample 2 302 286 265 296 287 12.34 4.3%
Sample 3 945 983 914 962 951 46.59 4.9%

*number of samples for each test n=16.


10ng/tube