Description
The Boster One Step Picokine™ Mouse Ifng Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Ifng in cell culture supernates and serum. It uses our proprietary One Step ELISA technology. One Step ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our One Step ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Mouse Ifng with immunogen: Expression system for standard: CHO; Immunogen sequence:Q283-R396.
To measure Mouse Ifng, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Mouse Ifng in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Mouse Ifng in the sample. For more information on assay principle, protocols, and troubleshooting tips, see Boster’s ELISA Resource Center at https://www.bosterbio.com/elisa-technical-resource-center.
Synonyms: Ifng; Interferon gamma; IFN-gamma
Species: Mouse Ifng
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <5pg/ml Specifity: Natural and recombinant Mouse Ifng
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies:
Anticoagulant: /
Immunogen: Expression system for standard: CHO; Immunogen sequence: Q283-R396
Gene name: Interferon gamma / interferon gamm
Protein name: Interferon gamma
Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|90|248|1038|92|258|860
Standard deviation|6.75|15.87|82.00|8.28|17.61|76.54
CV(%)|7.5%|6.4%|7.9%|9.0%|6.8%|8.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 90 | 97 | 92 | 86 | 91 | 3.96 | 4.3% |
| Sample 2 | 248 | 254 | 259 | 245 | 251 | 5.40 | 2.1% |
| Sample 3 | 1038 | 974 | 860 | 893 | 941 | 69.57 | 7.3% |
*number of samples for each test n=16.
10ng/tube
