Description
Boster’s rat GM-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GM-CSF has been precoated onto 96-well plates. Standards(E.coli, A1-K127) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GM-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat GM-CSF amount of sample captured in plate.
Synonyms: Colony stimulating factor|_x000D_Colony stimulating factor 2 (granulocyte macrophage)|_x000D_CSF|_x000D__x000D__x000D_CSF 2|_x000D_CSF2|_x000D_GMCSF|_x000D_Granulocyte macrophage colony stimulating factor|_x000D_MGC131935|_x000D_MGC138897|_x000D_Molgramostin|_x000D_Sargramostim
Species: Rat GM-CSF
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant rat GM-CSF
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, A1-K127
Gene name: Granulocyte-macrophage colony-stimulating factor / colony stimulating factor 2 (granulocyte-macrophage)
Protein name: Granulocyte-macrophage colony-stimulating factor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|30|216|429|28|196|414
Standard deviation|1.35|11.44|33.89|1.59|12.34|39.33
CV(%)|4.5%|5.3%|7.9%|5.7%|6.3%|9.5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 30 | 33 | 34 | 29 | 31 | 2.06 | 6.6% |
| Sample 2 | 216 | 227 | 243 | 239 | 231 | 10.59 | 4.5% |
| Sample 3 | 429 | 381 | 390 | 404 | 401 | 18.12 | 4.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0366-CAP|96-well plate precoated with anti- rat GM-CSF antibody|1
EK0366-ST|Lyophilized recombinant rat GM-CSF standard|10ng/tube×2
EK0366-DA|Biotinylated anti- rat GM-CSF antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
