Description
The Boster One Step Picokine™ Human TIMP3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human TIMP3 with a 96-well strip plate that is pre-coated with antibody specific for TIMP3. The detection antibody is a HRP conjugated antibody specific for TIMP3. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human TIMP3 with immunogen: Expression system for standard: NSO; Immunogen sequence: C24-P211. The kit is analytically validated with ready to use reagents.
To measure Human TIMP3, add standards and samples to the wells, then add HRP conjugated detection antibody. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human TIMP3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human TIMP3 in the sample. For more information on assay principle, protocols, and troubleshooting tips, see Boster’s ELISA Resource Center at https://www.bosterbio.com/elisa-technical-resource-center.
Synonyms: TIMP3; Metalloproteinase inhibitor 3; Protein MIG-5; Tissue inhibitor of metalloproteinases 3; TIMP-3
Species: Human TIMP3
Assay: 156pg/ml-10,000pg/ml
Sensitivy: <2pg/ml Specifity: Natural and recombinant Human TIMP3
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: monoclonal antibody from mouse|polyclonal antibody from goat
Anticoagulant: heparin, EDTA
Immunogen: Expression system for standard: NSO; Immunogen sequence: C24-P211
Gene name: Metalloproteinase inhibitor 3 / TIMP metallopeptidase inhibitor 3
Protein name: Metalloproteinase inhibitor 3
Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|228|1364|4394|244|1487|4066
Standard deviation|14.59|90.02|237.27|19.03|107.06|333.41
CV(%)|6.4%|6.6%|5.4%|7.8%|7.2%|8.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 228 | 257 | 260 | 256 | 250 | 11.25 | 4.5% |
| Sample 2 | 1364 | 1309 | 1336 | 1522 | 1382 | 81.53 | 5.9% |
| Sample 3 | 4394 | 4601 | 4288 | 4589 | 4468 | 285.95 | 6.4% |
*number of samples for each test n=16.
10ng/tube
