Description
The Boster One Step Picokine™ Mouse Tnf Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Tnf with a 96-well strip plate that is pre-coated with antibody specific for Tnf. The detection antibody is a HRP conjugated antibody specific for Tnf. The capture antibody is monoclonal antibody from hamster, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Tnf with immunogen: Expression system for standard: E.coli; Immunogen sequence: L80-L235 The kit is analytically validated with ready to use reagents.
To measure Mouse Tnf, add standards and samples to the wells, then add HRP conjugated detection antibody. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Mouse Tnf in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Mouse Tnf in the sample. For more information on assay principle, protocols, and troubleshooting tips, see Boster’s ELISA Resource Center at https://www.bosterbio.com/elisa-technical-resource-center.
Synonyms: Tnf; Tnfa; Tnfsf2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a [Cleaved into: Tumor necrosis factor, membrane form; N-terminal fragment; NTF; Intracellular domain 1; ICD1; Intracellular domain 2; ICD2; C-domain 1; C-domain 2; Tumor necrosis factor, soluble form]
Species: Mouse Tnf
Assay: 15.6pg/ml-1000pg/ml(cell culture supernates), 7.8pg/ml-500pg/ml(mouse serum, plasma)
Sensitivy: <1pg/ml Specifity: Natural and recombinant Mouse Tnf
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: monoclonal antibody from hamster|polyclonal antibody from goat
Anticoagulant: heparin, EDTA
Immunogen: Expression system for standard: E.coli; Immunogen sequence: L80-L235
Gene name: Tumor necrosis factor / tumor necrosis factor
Protein name: Tumor necrosis factor
Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|20|55|314|18|63|350
Standard deviation|1.04|3.74|23.55|1.06|4.59|28.7
CV(%)|5.2%|6.8%|7.5%|5.9%|7.3%|8.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 20 | 22 | 18 | 19 | 19 | 0.89 | 4.7% |
| Sample 2 | 55 | 58 | 64 | 57 | 58 | 3.65/td> | 6.3% |
| Sample 3 | 314 | 297 | 303 | 287 | 300 | 8.1 | 2.7% |
*number of samples for each test n=16.
10ng/tube
