Description
Boster’s rat CNTF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CNTF has been precoated onto 96-well plates. Standards(E.coli, A2-M200) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CNTF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CNTF amount of sample captured in plate.
Synonyms: AI429687|Ciliary Neurotrophic Factor|HCNTF|MGC41235|OTTHUMP00000174731|OTTMUSP00000029848
Species: Rat CNTF
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant rat CNTF
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: E.coli, A2-M200
Gene name: Ciliary neurotrophic factor / ciliary neurotrophic factor
Protein name: Ciliary neurotrophic factor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|82|276|868|80|293|809
Standard deviation|4.59|16.83|60.76|5.52|23.14|60.67
CV(%)|5.6%|6.1%|7%|6.9%|7.9%|7.5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 82 | 75 | 85 | 81 | 80 | 3.63 | 4.5% |
| Sample 2 | 276 | 262 | 311 | 268 | 279 | 18.99 | 6.8% |
| Sample 3 | 868 | 1021 | 877 | 993 | 939 | 68.04 | 7.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0324-CAP|96-well plate precoated with anti- rat CNTF antibody|1
EK0324-ST|Lyophilized recombinant rat CNTF standard|10ng/tube×2
EK0324-DA|Biotinylated anti- rat CNTF antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
