Description
Boster’s rat CD80 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD80 has been precoated onto 96-well plates. Standards(NSO, I39-Q248) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD80 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CD80 amount of sample captured in plate.
Synonyms: Activation B7-1 antigen|B7|BB1|CD80|CD80_HUMAN|CTLA-4 counter-receptor B7.1|T-lymphocyte activation antigen CD80
Species: Rat CD80
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <2pg/ml Specifity: Natural and recombinant rat CD80
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: NSO, I39-Q248
Gene name: T-lymphocyte activation antigen CD80 / CD80 molecule
Protein name: T-lymphocyte activation antigen CD80
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|154|448|1666|165|472|1688
Standard deviation|10.78|21.5|89.96|13.69|29.26|113.09
CV(%)|7%|4.8%|5.4%|8.3%|6.2%|6.7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 154 | 156 | 159 | 178 | 161 | 9.54 | 5.9% |
| Sample 2 | 448 | 401 | 380 | 439 | 417 | 27.7 | 6.6% |
| Sample 3 | 1666 | 1734 | 1536 | 1629 | 1641 | 71.48 | 4.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0709-CAP|96-well plate precoated with anti- rat CD80 antibody|1
EK0709-ST|Lyophilized recombinant rat CD80 standard|10ng/tube×2
EK0709-DA|Biotinylated anti- rat CD80 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
