Description
Boster’s mouse TFF1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TFF1 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: Q22-F87) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TFF1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TFF1 amount of sample captured in plate.
Synonyms: Trefoil factor 1|Protein pS2|Tff1|Bcei|Ps2
Species: Mouse Tff1
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse TFF1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, Q22-F87
Gene name: Trefoil factor 1 / trefoil factor 1
Protein name: Trefoil factor 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|52|244|807|47|265|781
Standard deviation|3.9|11.95|42.77|4.32|14.57|53.88
CV(%)|7.5%|4.9%|5.3%|9.2%|5.5%|6.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 52 | 60 | 62 | 62 | 59 | 4.12 | 6.9% |
| Sample 2 | 244 | 254 | 259 | 240 | 249 | 7.59 | 3% |
| Sample 3 | 807 | 768 | 839 | 743 | 789 | 36.67 | 4.6% |
*number of samples for each test n=16.
Catalog number|Description|Quantity_x000D_
EK1620-CAP|96-well plate precoated with anti- mouse TFF1 antibody|1_x000D_
EK1620-ST|Lyophilized recombinant mouse TFF1 standard|10ng/tube×2_x000D_
EK1620-DA|Biotinylated anti- mouse TFF1 antibody|130μl(dilution 1:100)_x000D_
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)_x000D_
AR1106-1|Sample diluent buffer|30ml_x000D_
AR1106-2|Antibody diluent buffer|12ml_x000D_
AR1106-3|ABC diluent buffer|12ml_x000D_
AR1104|TMB color developing agent|10ml_x000D_
AR1105|TMB stop solution|10ml_x000D_
PLA-SEA|Adhesive cover|4
