Description
Boster’s mouse SerpinF2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SerpinF2 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: N40-K491） and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SerpinF2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SerpinF2 amount of sample captured in plate.
Synonyms: Alpha-2-antiplasmin; Alpha-2-AP; Alpha-2-plasmin inhibitor; Alpha-2-PI; Serpin F2; Serpinf2; Pli
Species: Mouse SerpinF2
Assay: 1.56ng/ml-100ng/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse GPC3
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: NSO,N40-K491
Gene name: Alpha-2-antiplasmin / serine (or cysteine) peptidase inhibitor, clade F, member 2
Protein name: Alpha-2-antiplasmin
Storage: Store at 4˚C for 6 months, at -20˚C for 15 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|2710|13864|42376|2900|14200|45464
Standard deviation|113.82|804.1|2712.06|130.5|965.6|3273.4
CV(%)|4.2%|5.8%|6.4%|4.5%|6.8%|7.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 2710 | 2230 | 2706 | 2277 | 2480 | 227.86 | 9.1% |
| Sample . 2 |
13864 | 12175 | 13380 | 12105 | 12881 | 760.90 | 5.9%% |
| Sample 3 | 42376 | 42947 | 47567 | 46317 | 44801 | 2194.70 | 4.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1713-CAP|96-well plate precoated with anti- mouse SerpinF2 antibody|1
EK1713-ST|Lyophilized recombinant mouse SerpinF2 standard|100ng/tubex2
EK1713-DA|Biotinylated anti- mouse SerpinF2 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
