Description
Boster’s mouse IFN gamma ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IFN gamma has been precoated onto 96-well plates. Standards(E.coli, H23-C155) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IFN gamma is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IFN gamma amount of sample captured in plate.
Synonyms: IFG|_x000D_IFI|_x000D_IFN gamma|_x000D__x000D__x000D_IFN Immune|_x000D_IFNG|_x000D_Interferon gamma|_x000D_Interferon Gamma Precursor|_x000D_Macrophage Activating Factor|_x000D_MAF|_x000D_T Cell Interferon|_x000D_Type II Interferon
Species: Mouse IFN gamma
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <5pg/ml Specifity: Natural and recombinant mouse IFN gamma
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: E.coli, H23-C155
Gene name: Interferon gamma / interferon, gamma
Protein name: Interferon gamma
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|68|224|760|70|232|686
Standard deviation|2.92|12.32|33.44|3.08|12.76|30.18
CV(%)|4.3%|5.5%|4.4%|4.4%|5.5%|4.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 68 | 69 | 66 | 65 | 67 | 1.58 | 2.3% |
| Sample 2 | 224 | 203 | 208 | 243 | 219 | 15.62 | 7.1% |
| Sample 3 | 760 | 839 | 731 | 883 | 803 | 60.68 | 7.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0375-CAP|96-well plate precoated with anti- mouse IFN gamma antibody|1
EK0375-ST|Lyophilized recombinant mouse IFN gamma standard|10ng/tube×2
EK0375-DA|Biotinylated anti- mouse IFN gamma antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
