Description
Boster’s mouse CLEC11A/SCGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CLEC11A/SCGF has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: A22-F328) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CLEC11A/SCGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Synonyms: C-type lectin domain family 11 member A; Lymphocyte secreted C-type lectin; Osteolectin; Stem cell growth factor; Clec11a; Scgf
Species: Mouse CLEC11A/SCGF
Assay: 0.625ng/ml-40ng/ml
Sensitivy: <0.1ng/ml Specifity: Natural and recombinant mouse CLEC11A/SCGF
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated detection polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, A22-F328
Gene name: C-type lectin domain family 11 member A / C-type lectin domain family 11, member a
Protein name: C-type lectin domain family 11 member A
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|1007|4278|23664|1209|4384|24959
Standard deviation|48.34|286.63|1254.19|68.91|188.51|1098.20
CV(%)|4.8%|6.7%|5.3%|5.7%|4.3%|4.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1007 | 1034 | 1004 | 1209 | 1063 | 84.81 | 7.9% |
| Sample 2 | 4278 | 4424 | 3789 | 4180 | 4167 | 235.27 | 5.6% |
| Sample 3 | 23664 | 25242 | 24959 | 25402 | 24816 | 684.18 | 2.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1947-CAP|96-well plate precoated with anti- mouse CLEC11A/SCGF antibody|1
EK1947-ST|Lyophilized recombinant mouse CLEC11A/SCGF standard|40ng/tube×2
EK1947-DA|Biotinylated anti- mouse CLEC11A/SCGF antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
