Description
Boster’s mouse beta IG-H3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for beta IG-H3 has been precoated onto 96-well plates. Standards(NSO, G24-H683) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for beta IG-H3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse beta IG-H3 amount of sample captured in plate.
Synonyms: Beta ig | Beta ig h3 | Beta ig-h3 | Big h3 | BIGH3 | CDB1 | CDG2 | CDGG1 | CSD | CSD1 | CSD2 | CSD3 | EBMD | Kerato epithelin | Kerato-epithelin | LCD1 | RGD CAP | RGD-CAP | TGFBI | Q15582
Species: mouse beta IG-H3
Assay: 156pg/ml-10000pg/ml
Sensitivy: <5pg/ml Specifity: Natural and recombinant mouse beta IG-H3
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, G24-H683
Gene name: Transforming growth factor-beta-induced protein ig-h3 / transforming growth factor, beta-induced, 68kDa
Protein name: Transforming growth factor-beta-induced protein ig-h3
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|301|1595|5482|302|1576|5124
Standard deviation|21.37|95.7|241.2|22.65|100.86|312.56
CV(%)|7.1%|6%|4.4%|7.5%|6.4%|6.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 301 | 315 | 329 | 290 | 308 | 14.66 | 4.7% |
| Sample 2 | 1595 | 1475 | 1467 | 1429 | 1491 | 62.23 | 4.1% |
| Sample 3 | 5482 | 6340 | 6191 | 6242 | 6063 | 340.11 | 5.6% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1191-CAP|96-well plate precoated with anti- mouse beta IG-H3 antibody|1
EK1191-ST|Lyophilized recombinant mouse beta IG-H3 standard|10ng/tube×2
EK1191-DA|Biotinylated anti- mouse beta IG-H3 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
