Description
Boster’s human TIM-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TIM-3 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: S22-G202) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIM-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TIM-3 amount of sample captured in plate.
Synonyms: Hepatitis A virus cellular receptor 2; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD-3; T-cell immunoglobulin mucin receptor 3; TIM-3; T-cell membrane protein 3; HAVCR2; TIM3; TIMD3
Species: Human TIM-3/HAVCR2
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human TIM-3
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO,S22-G202
Gene name: Hepatitis A virus cellular receptor 2 / hepatitis A virus cellular receptor 2
Protein name: Hepatitis A virus cellular receptor 2
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|51|224|977|50|216|884
Standard deviation|2.44|10.08|68.39|2.55|10.36|74.25
CV(%)|4.8%|4.5%|7%|5.1%|4.8%|8.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 51 | 57 | 53 | 50 | 52 | 2.68 | 5.1% |
| Sample 2 | 224 | 222 | 238 | 249 | 233 | 10.98 | 4.7% |
| Sample 3 | 977 | 1070 | 1006 | 898 | 987 | 61.78 | 6.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity_x000D_
EK1644-CAP|96-well plate precoated with anti- human TIM-3 antibody|1
EK1644-ST|Lyophilized recombinant human TIM-3 standard|10ng/tube×2
EK1644-DA|Biotinylated anti- human TIM-3 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)_x000D_
AR1106-1|Sample diluent buffer|30ml_x000D_
AR1106-2|Antibody diluent buffer|12ml_x000D_
AR1106-3|ABC diluent buffer|12ml_x000D_
AR1104|TMB color developing agent|10ml_x000D_
AR1105|TMB stop solution|10ml_x000D_
PLA-SEA|Adhesive cover|4
