Description
Boster’s human TGFBR2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for TGFBR2 has been precoated onto 96-well plates. Standards(NSO, I24-D159) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGFBR2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TGFBR2 amount of sample captured in plate.
Synonyms: AAT3|FAA3|LDS1B|LDS2|LDS2B|MFS2|RIIC|TAAD2|TbetaR-II|TGF-beta receptor type II|TGF-beta receptor type-2|TGF-beta type II receptor|TGFbeta RII|Tgfbr2|TGFR 2|TGFR-2|TGFR2_HUMAN|Transforming growth factor beta receptor II (70/80kDa)|Transforming growth factor-beta receptor type II
Species: human TGFBR2
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human TGFBR2
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, I24-D159
Gene name: TGF-beta receptor type-2 / transforming growth factor, beta receptor II (70/80kDa)
Protein name: TGF-beta receptor type-2
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|33|179|421|30|166|392
Standard deviation|2.34|8.23|18.1|2.31|8.3|21.16
CV(%)|7.1%|4.6%|4.3%|7.7%|5%|5.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 33 | 34 | 33 | 36 | 34 | 1.22 | 3.5% |
| Sample 2 | 179 | 177 | 172 | 160 | 172 | 7.38 | 4.2% |
| Sample 3 | 421 | 443 | 453 | 438 | 438 | 11.58 | 2.6% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1307-CAP|96-well plate precoated with anti- human TGFBR2 antibody|1
EK1307-ST|Lyophilized recombinant human TGFBR2 standard|10ng/tube×2
EK1307-DA|Biotinylated anti- human TGFBR2 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
