Description
Boster’s human CD5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for CD5 has been precoated onto 96-well plates. Standards(NSO, R25-N371) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD5 amount of sample captured in plate.
Synonyms: CD 5|CD5|CD5 antigen (p56 62)|CD5 antigen|CD5 molecule|CD5_HUMAN|LEU 1|LEU1|Ly12|LyA|Lymphocyte Antigen CD5|Lymphocyte antigen T1/Leu 1|Lymphocyte antigen T1/Leu-1|Lymphocyte glycoprotein T1/Leu1|OTTHUMP00000236973|p56 62|T cell surface glycoprotein CD5|T-cell surface glycoprotein CD5|T1
Species: human CD5
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CD5
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, R25-N371
Gene name: T-cell surface glycoprotein CD5 / CD5 molecule
Protein name: T-cell surface glycoprotein CD5
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|32|263|425|33|253|425
Standard deviation|1.69|11.04|28.05|2.27|15.18|30.6
CV(%)|5.3%|4.2%|6.6%|6.9%|6%|7.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 32 | 32 | 34 | 33 | 32 | 0.82 | 2.5% |
| Sample 2 | 263 | 259 | 241 | 228 | 247 | 14.09 | 5.7% |
| Sample 3 | 425 | 469 | 430 | 397 | 430 | 25.66 | 5.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1220-CAP|96-well plate precoated with anti- human CD5 antibody|1
EK1220-ST|Lyophilized recombinant human CD5 standard|10ng/tube×2
EK1220-DA|Biotinylated anti- human CD5 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
