Description
Boster’s human MyD88 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MyD88 has been precoated onto 96-well plates. Standards(E.coli, M1-P296) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MyD88 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MyD88 amount of sample captured in plate.
Synonyms: Myd88|_x000D_MYD88_HUMAN|_x000D_MYD88D|_x000D__x000D__x000D_myeloid differentiation primary response gene (88)|_x000D_Myeloid differentiation primary response protein MyD88
Species: Human MyD88
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human MyD88
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, M1-P296
Gene name: Myeloid differentiation primary response protein MyD88 / myeloid differentiation primary response 88
Protein name: Myeloid differentiation primary response protein MyD88
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|244|985|5638|235|1056|5584
Standard deviation|19.27|61.07|332.64|21.15|76.03|429.96
CV(%)|7.9%|6.2%|5.9%|9%|7.2%|7.7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 244 | 207 | 232 | 211 | 223 | 15.17 | 6.8% |
| Sample 2 | 985 | 915 | 1056 | 940 | 974 | 53.57 | 5.5% |
| Sample 3 | 5638 | 5953 | 5999 | 5631 | 5805 | 171.54 | 2.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0942-CAP|96-well plate precoated with anti- human MyD88 antibody|1
EK0942-ST|Lyophilized recombinant human MyD88 standard|10ng/tube×2
EK0942-DA|Biotinylated anti- human MyD88 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
