Description
Boster’s human MMP-12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-12 has been precoated onto 96-well plates. Standards(NSO, L17-C470) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MMP-12 amount of sample captured in plate.
Synonyms: EC 3.4.24.65|HME|Macrophage elastase|Macrophage metalloelastase|Macrophage metaloelastase|Matrix metallopeptidase 12 (macrophage elastase)|Matrix metalloprotease 12|Matrix metalloproteinase-12|MGC138506|MME|MMP 12|MMP-12|Mmp12|MMP12_HUMAN
Species: Human MMP-12
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human MMP-12
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin
Immunogen: NSO, L17-C470
Gene name: Macrophage metalloelastase / matrix metallopeptidase 12 (macrophage elastase)
Protein name: Macrophage metalloelastase
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|201|499|1029|197|507|1084
Standard deviation|12.86|20.45|59.68|13.39|30.42|62.87
CV(%)|6.4%|4.1%|5.8%|6.8%|6%|5.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 201 | 172 | 181 | 197 | 187 | 11.77 | 6.2% |
| Sample 2 | 499 | 501 | 511 | 504 | 503 | 4.54 | 0.9% |
| Sample 3 | 1029 | 985 | 961 | 957 | 983 | 28.63 | 2.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0950-CAP|96-well plate precoated with anti- human MMP-12 antibody|1
EK0950-ST|Lyophilized recombinant human MMP-12 standard|10ng/tube×2
EK0950-DA|Biotinylated anti- human MMP-12 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
