Description
Boster’s human MBP-C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MBP-C has been precoated onto 96-well plates. Standards(NSO, E21-I248) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MBP-C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MBP-C amount of sample captured in plate.
Synonyms: COLEC 1|COLEC1|Collectin-1|HSMBPC|Lectin, mannose-binding, soluble, 2|Mannan binding lectin|Mannan binding protein|Mannan-binding protein|Mannose binding lectin (protein C) 2 soluble|Mannose binding lectin (protein C) 2, soluble (opsonic defect)|Mannose binding lectin (protein C) 2, soluble|Mannose binding lectin 2 soluble|Mannose binding lectin 2, soluble (opsonic defect)|Mannose binding lectin|Mannose binding lectin protein C2 soluble opsonic defect|Mannose binding protein|Mannose binding protein C|Mannose binding protein C precursor|Mannose binding protein, serum|Mannose-binding lectin|Mannose-binding protein C|MBL 2|MBL|MBL2|MBL2_HUMAN|MBL2D|MBP 1|MBP|MBP C|MBP-C|MBP1|MBPB|MBPC|MBPD|MGC116832|MGC116833|Opsonic defect|protein C|Soluble mannose binding lectin
Species: Human MBP-C
Assay: 312pg/ml-20000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human MBP-C
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, E21-I248
Gene name: Mannose-binding protein C / mannose-binding lectin (protein C) 2, soluble
Protein name: Mannose-binding protein C
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|479|2934|8702|458|2785|8839
Standard deviation|27.3|129.09|661.35|34.35|147.6|742.47
CV(%)|5.7%|4.4%|7.6%|7.5%|5.3%|8.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 479 | 546 | 475 | 475 | 493 | 30.21 | 6.1% |
| Sample 2 | 2934 | 2910 | 2938 | 2814 | 2899 | 50.22 | 1.7% |
| Sample 3 | 8702 | 9816 | 8527 | 9384 | 9107 | 519.57 | 5.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0805-CAP|96-well plate precoated with anti- human MBP-C antibody|1
EK0805-ST|Lyophilized recombinant human MBP-C standard|20ng/tube×2
EK0805-DA|Biotinylated anti- human MBP-C antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
