Description
Boster’s human FOLR1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FOLR1 has been precoated onto 96-well plates. Standards(E.coli, R25-S234) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FOLR1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FOLR1 amount of sample captured in plate.
Synonyms: Adult folate binding protein|Adult folate-binding protein|FBP|Folate Receptor 1 Adult|Folate receptor 1|Folate Receptor 1 Precursor|Folate receptor adult|Folate receptor alpha|Folate receptor|FOLR|FOLR1|FOLR1_HUMAN|FR alpha|FR-alpha|FRalpha|KB cells FBP|MOV18|Ovarian cancer associated antigen|Ovarian tumor associated antigen|Ovarian tumor associated antigen MOv18|Ovarian tumor-associated antigen MOv18
Species: human FOLR1
Assay: 46.9pg/ml-3000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human FOLR1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, R25-S234
Gene name: Folate receptor alpha / folate receptor 1 (adult)
Protein name: Folate receptor alpha
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|103|333|1306|108|312|1388
Standard deviation|7.41|22.31|88.8|8.53|24.96|119.36
CV(%)|7.2%|6.7%|6.8%|7.9%|8%|8.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 103 | 108 | 100 | 99 | 102 | 3.5 | 3.4% |
| Sample 2 | 333 | 359 | 355 | 339 | 346 | 10.8 | 3.1% |
| Sample 3 | 1306 | 1386 | 1411 | 1506 | 1402 | 71.35 | 5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1242-CAP|96-well plate precoated with anti- human FOLR1 antibody|1
EK1242-ST|Lyophilized recombinant human FOLR1 standard|10ng/tube×2
EK1242-DA|Biotinylated anti- human FOLR1 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
