Description
Boster’s human Eotaxin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Eotaxin has been precoated onto 96-well plates. Standards(E.coli, G24-P97) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Eotaxin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Eotaxin amount of sample captured in plate.
Synonyms: C-C motif chemokine 11|CCL 11|CCL11|CCL11_HUMAN|Chemokine (C-C motif) ligand 11|Chemokine C C motif ligand 11|Chemokine CC Motif Ligand 11|Chemokine ligand 11|Eosinophil Chemotactic P|Eosinophil chemotactic protein|Eotaxin|Eotaxin 1|MGC22554|SCYA 11|SCYA11|Small inducible cytokine A11|Small inducible cytokine subfamily A (Cys Cys) member 11|Small inducible cytokine subfamily A member 11|Small-inducible cytokine A11
Species: Human Eotaxin
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <2pg/ml Specifity: Natural and recombinant human Eotaxin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: citrate or EDTA
Immunogen: E.coli, G24-P97
Gene name: Eotaxin / chemokine (C-C motif) ligand 11
Protein name: Eotaxin
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|36|181|476|34|176|517
Standard deviation|1.8|13.39|37.6|1.93|15.84|41.87
CV(%)|5%|7.4%|7.9%|5.7%|9%|8.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 36 | 31 | 34 | 34 | 33 | 1.78 | 5.3% |
| Sample 2 | 181 | 203 | 202 | 192 | 194 | 8.9 | 4.5% |
| Sample 3 | 476 | 507 | 504 | 491 | 494 | 12.25 | 2.4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0329-CAP|96-well plate precoated with anti- human Eotaxin antibody|1
EK0329-ST|Lyophilized recombinant human Eotaxin standard|10ng/tube×2
EK0329-DA|Biotinylated anti- human Eotaxin antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
