Description
Boster’s human DAN ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DAN has been precoated onto 96-well plates. Standards (NSO, A17-D181) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DAN is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DAN amount of sample captured in plate.
Synonyms: D1S1733E|DAN|DAN domain family member 1|DAND 1|DAND1|Differential screening selected gene aberrant in neuroblastoma|MGC8972|NB|NBL 1|Neuroblastoma candidate region suppression of tumorigenicity 1|Neuroblastoma suppression of tumorigenicity 1|Neuroblastoma suppressor of tumorigenicity 1|NO 3|NO3|Zinc finger protein DAN
Species: Human DAN
Assay: 125pg/ml-8000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human DAN
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, A17-D181
Gene name: Neuroblastoma suppressor of tumorigenicity 1 / neuroblastoma 1, DAN family BMP antagonist
Protein name: Neuroblastoma suppressor of tumorigenicity 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|158|931|5529|160|963|5394
Standard deviation|9.63|70.75|293.03|10.08|75.11|302.06
CV(%)|6.1%|7.6%|5.3%|6.3%|7.8%|5.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 158 | 176 | 186 | 157 | 169 | 12.27 | 7.2% |
| Sample 2 | 931 | 1035 | 907 | 1065 | 984 | 66.89 | 6.7% |
| Sample 3 | 5529 | 5905 | 6039 | 5194 | 5666 | 330.83 | 5.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0746-CAP|96-well plate precoated with anti- human DAN antibody|1
EK0746-ST|Lyophilized recombinant human DAN standard|10ng/tube×2
EK0746-DA|Biotinylated anti- human DAN antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
