Description
Boster’s human CD48 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD48 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: Q27-S220) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD48 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD48 amount of sample captured in plate.
Synonyms: Antigen CD48 | BCM 1 surface antigen | Bcm-1 | BCM1 surface antigen | BCM1, BLAST1 | BLAST | CD48 antigen | CD48 molecule | CD48 protein | Leucocyte antigen MEM 102 | Leukocyte antigen MEM-102 | MEM102 | SLAMF2 | TCT.1 | P09326
Species: human CD48
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <12pg/ml Specifity: Natural and recombinant human CD48
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, Q27-S220
Gene name: CD48 antigen / CD48 molecule
Protein name: CD48 antigen
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|113|615|2155|117|667|2219
Standard deviation|5.53|46.12|129.3|6.66|58.69|157.54
CV(%)|4.9%|7.5%|6%|5.7%|8.8%|7.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 113 | 100 | 101 | 104 | 104 | 5.12 | 4.9% |
| Sample 2 | 615 | 601 | 670 | 606 | 623 | 27.59 | 4.4% |
| Sample 3 | 2155 | 1979 | 1842 | 1829 | 1951 | 131.49 | 6.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1527-CAP|96-well plate precoated with anti- human CD48 antibody|1
EK1527-ST|Lyophilized recombinant human CD48 standard|10ng/tube×2
EK1527-DA|Biotinylated anti- human CD48 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
