Description
Boster’s human CD80 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD80 has been precoated onto 96-well plates. Standards(NSO, V35-N242) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for CD80 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD80 amount of sample captured in plate.
Synonyms: T-lymphocyte activation antigen CD80;Activation B7-1 antigen;BB1;CTLA-4 counter-receptor B7.1;B7;CD80;CD80;CD28LG, CD28LG1, LAB7;
Species: Human B7-1
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CD80
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: monoclonal antibody from mouse|monoclonal antibody from mouse
Anticoagulant:
Immunogen: Expression system for standard: NSO; Immunogen sequence: V35-N242
Gene name: T-lymphocyte activation antigen CD80 / T-lymphocyte activation antigen CD80
Protein name: T-lymphocyte activation antigen CD80
Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 162 | 445 | 1656 | 163 | 425 | 1791 |
| Standard deviation | 7.29 | 34.71 | 69.55 | 7.98 | 34 | 100.29 |
| CV(%) | 4.5% | 7.8% | 4.2% | 4.9% | 8% | 5.6% |
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 162 | 138 | 164 | 139 | 150 | 12.27 | 8.1% |
| Sample 2 | 445 | 495 | 515 | 457 | 478 | 28.23 | 5.9% |
| Sample 3 | 1656 | 1667 | 1402 | 1560 | 1571 | 106.21 | 6.7% |
*number of samples for each test n=16.
10ng/tube
