Description
Boster’s human APP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for APP has been precoated onto 96-well plates. Standards(NSO, L18-L688) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for APP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human APP amount of sample captured in plate.
Synonyms: A4 amyloid protein|A4|A4_HUMAN|AAA|ABETA|ABPP|AD 1|AD1|AICD-50|AICD-57|AICD-59|AID(50)|AID(57)|AID(59)|Alzheimer disease 1|Alzheimer disease amyloid protein|Alzheimer’s disease amyloid protein|Amyloid beta (A4) precursor protein|Amyloid beta A4 protein|Amyloid beta A4 protein precursor isoform b|Amyloid beta A4 protein precursor isoform b|Amyloid beta A4 protein precursor isoform c|Amyloid beta A4 protein precursor isoform c|Amyloid beta A4 protein precursor isoform a|Amyloid beta A4 protein precursor isoform b|Amyloid beta A4 protein precursor isoform b|Amyloid beta A4 protein precursor isoform c|Amyloid beta A4 protein precursor isoform c|Amyloid beta protein|Amyloid beta-peptide|Amyloid intracellular domain 50|Amyloid intracellular domain 57|Amyloid intracellular domain 59|Amyloid of aging and alzheimer disease|APP|APP I|APPI|Beta amyloid peptide|Beta-APP40|Beta-APP42|C31|Cerebral vascular amyloid peptide|CTFgamma|CVAP|Gamma-CTF(50)|Gamma-CTF(57)|Gamma-CTF(59)|Human mRNA for amyloid A4 precursor of Alzheimer’s disease|peptidase nexin II|PN 2|PN II|PN-II|PN2|PreA4|Protease nexin II|Protease nexin-II|S-APP-alpha|S-APP-beta
Species: Human APP
Assay: 312pg/ml-20000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human APP
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, L18-L688
Gene name: Amyloid beta A4 protein / amyloid beta (A4) precursor protein
Protein name: Amyloid beta A4 protein
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|775|2619|9426|755|2868|9446
Standard deviation|50.37|188.56|377.04|54.36|226.57|510.08
CV(%)|6.5%|7.2%|4%|7.2%|7.9%|5.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 775 | 742 | 738 | 700 | 738 | 26.58 | 3.6% |
| Sample 2 | 2619 | 3033 | 2635 | 3053 | 2835 | 208.19 | 7.3% |
| Sample 3 | 9426 | 9494 | 8945 | 9504 | 9342 | 231.3 | 2.4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0658-CAP|96-well plate precoated with anti- human APP antibody|1
EK0658-ST|Lyophilized recombinant human APP standard|20ng/tubex2
EK0658-DA|Biotinylated anti- human APP antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
