ELISA Kits

Showing 3151–3300 of 3623 results

  • For quantitative detection of rat Plexin A1 in cell culture supernates, serum and plasma (heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK1819

    Available on backorder

  • For quantitative detection of rat Plexin B2 in cell culture supernates, serum and plasma (heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK1883

    Available on backorder

  • For Quantitative Detection of rat CXCL7 in cell culture supernates, serum and plasma (heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK0731

    Available on backorder

  • For quantitative detection of rat RAGE in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK0971

    Available on backorder

  • For quantitative detection of rat RANK in cell culture supernates and serum.   

     

    Brand:
    Boster Bio
    SKU:EK1281

    Available on backorder

  • The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Rat Rank/TNFRSF11A with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates and serum. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Rank/TNFRSF11A tissue specificity:   

     

    Brand:
    Boster Bio
    SKU:FEK1281

    Available on backorder

  • For quantitative detection of rat Rantes in cell culture supernates, serum and plasma(heparin, EDTA, citrate).   

     

    Brand:
    Boster Bio
    SKU:EK0496

    Available on backorder

  • For quantitative detection of rat REG3A in cell culture supernates, serum and plasma (heparin).   

     

    Brand:
    Boster Bio
    SKU:EK1836

    Available on backorder

  • For quantitative detection of rat S100B in cell culture supernates, serum and plasma(heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK1165

    Available on backorder

  • For quantitative detection of rat SAP in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK1209

    Available on backorder

  • For quantitative detection of rat SCF in cell culture supernates, serum and plasma(EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK0508

    Available on backorder

  • For Quantitative Detection of rat SCGB1A1 in cell culture supernates, serum, plasma (heparin, EDTA) and urine.   

     

    Brand:
    Boster Bio
    SKU:EK1636

    Available on backorder

  • Sandwich High Sensitivity ELISA kit for Quantitative The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detection of rat SCGB1A1. 96wells/kit, with removable strips.   

     

    Brand:
    Boster Bio
    SKU:FEK1636

    Available on backorder

  • For quantitative detection of rat SCGB3A1 in cell culture supernates, serum and plasma (heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK1994

    Available on backorder

  • For quantitative detection of rat Sclerostin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK1565

    Available on backorder

  • For quantitative detection of rat soluble E-Selectin in cell culture supernates, serum and plasma(heparin, citrate).   

     

    Brand:
    Boster Bio
    SKU:EK0936

    Available on backorder

  • The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Rat E Selectin/SELE with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. E Selectin/SELE tissue specificity:   

     

    Brand:
    Boster Bio
    SKU:FEK0936

    Available on backorder

  • For quantitative detection of rat SEMA3F in cell culture supernates, serum and plasma (heparin, EDTA, citrate).   

     

    Brand:
    Boster Bio
    SKU:EK1735

    Available on backorder

  • For quantitative detection of rat SEMA4G in cell culture supernates, serum and plasma (heparin, EDTA, citrate).   

     

    Brand:
    Boster Bio
    SKU:EK2055

    Available on backorder

  • For quantitative detection of rat SEMA6A in cell culture supernates, serum and plasma (heparin, EDTA, citrate).   

     

    Brand:
    Boster Bio
    SKU:EK2057

    Available on backorder

  • For quantitative detection of rat SEMA6C in cell culture supernates, serum and plasma (heparin, EDTA, citrate).   

     

    Brand:
    Boster Bio
    SKU:EK2059

    Available on backorder

  • For quantitative detection of rat Serpin A12 in cell culture supernates, serum and plasma (heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK1741

    Available on backorder

  • For quantitative detection of rat Serpin D1 in cell culture supernates, serum and plasma (EDTA, citrate) and urine.   

     

    Brand:
    Boster Bio
    SKU:EK1970

    Available on backorder

  • For quantitative detection of rat SerpinE2 in cell culture supernates, serum and plasma (heparin, EDTA, citrate) and urine.   

     

    Brand:
    Boster Bio
    SKU:EK1886

    Available on backorder

  • For quantitative detection of rat SEZ6L in cell culture supernates, serum and plasma (heparin).   

     

    Brand:
    Boster Bio
    SKU:EK1832

    Available on backorder

  • For quantitative detection of rat soluble L-Selectin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK0937

    Available on backorder

  • For quantitative detection of rat SMOC1 in cell culture supernates, serum and plasma (heparin).   

     

    Brand:
    Boster Bio
    SKU:EK1937

    Available on backorder

  • For quantitative detection of rat STC2 in cell culture supernates, serum and plasma (heparin).   

     

    Brand:
    Boster Bio
    SKU:EK1995

    Available on backorder

  • For Quantitative Detection of rat TEK in cell culture supernates, serum and plasma (heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK1683

    Available on backorder

  • For Quantitative Detection of rat TFF3 in cell culture supernates, serum and plasma (heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK1679

    Available on backorder

  • For quantitative detection of activated rat TGF beta 1 in cell culture supernates, serum, plasma(EDTA) and urine.   

     

    Brand:
    Boster Bio
    SKU:EK0514

    Available on backorder

  • For quantitative detection of activated rat TGF-beta 2 in cell culture supernates, serum and plasma(heparin, EDTA, citrate).   

     

    Brand:
    Boster Bio
    SKU:EK0982

    Available on backorder

  • For quantitative detection of activated rat TGF-beta 3 in cell culture supernates, serum, plasma(EDTA) and urine.   

     

    Brand:
    Boster Bio
    SKU:EK1105

    Available on backorder

  • For quantitative detection of rat TIMP-1 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK0583

    Available on backorder

  • For quantitative detection of rat TIMP-2 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK1302

    Available on backorder

  • For quantitative detection of rat TNF alpha in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK0526

    Available on backorder

  • For quantitative detection of rat TNFRSF19 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK1607

    Available on backorder

  • For quantitative detection of rat TNFSF11 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK1559

    Available on backorder

  • For quantitative detection of rat TNFSF12 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK1177

    Available on backorder

  • For quantitative detection of rat TNFSF14 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK1575

    Available on backorder

  • For quantitative detection of rat TrkA in cell culture supernates and cell lysates.   

     

    Brand:
    Boster Bio
    SKU:EK0847

    Available on backorder

  • For quantitative detection of rat TRKB in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK1596

    Available on backorder

  • For Quantitative Detection of rat TRKC in cell culture supernates and cell lysates.   

     

    Brand:
    Boster Bio
    SKU:EK1660

    Available on backorder

  • For quantitative detection of rat VE-Cadherin/CD144 in cell culture supernates, serum and plasma (heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK1916

    Available on backorder

  • For quantitative detection of rat VEGF in cell culture supernates, serum and plasma(heparin, EDTA, citrate).   

     

    Brand:
    Boster Bio
    SKU:EK0540

    Available on backorder

  • For quantitative detection of rat VEGFR3 in cell culture supernates and serum.   

     

    Brand:
    Boster Bio
    SKU:EK1347

    Available on backorder

  • For quantitative detection of rat WIF1 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK1564

    Available on backorder

  • For quantitative detection of rat WISP1 in cell culture supernates, serum and plasma (heparin, EDTA).   

     

    Brand:
    Boster Bio
    SKU:EK1588

    Available on backorder

  • Brand:
    Cayman
    SKU:700992 - 6 mg

    Available on backorder

  • Brand:
    Cayman
    SKU:10006870 - 1 ea

    Available on backorder

  • Renin is an aspartyl protease of approximately 40 kDa that is released in active form from renal juxtaglomerular cells in response to sodium depletion, decreased blood volume and blood pressure, and b-adrenergic stimulation.{12331,13007,12970} Renin catalyzes the initial and rate limiting step in the renin-angiotensin system (RAS) pathway, converting angiotensinogen into angiotensin I. Angiotensin Converting Enzyme (ACE) subsequently converts angiotensin I to angiotensin II, which is a potent vasoconstrictor. Cayman’s Renin Inhibitor Screening Assay Kit provides a convenient assay in a 96-well format for evaluating human renin inhibitors. The assay utilizes a renin-based synthetic peptide substrate which incorporates the fluorophore EDANS at one end and an EDAN-quenching molecule (Dabcyl) at the other end.{12969} After cleavage by renin, the peptide-EDANS product is released yielding bright fluorescence which can be easily analyzed using excitation wavelengths of 335-345 nm and emission wavelengths of 485-510 nm. The assay kit includes recombinant human renin (sufficient for 100 reactions), substrate, buffers, and complete instructions.  

     

    Brand:
    Cayman
    SKU:10006270 - 96 wells

    Available on backorder

  • Cayman’s Renin Monoclonal Antibody (Clone 3G10) is directed against the full length renin (human recombinant) protein (Item No. 10006217). Renin is an aspartyl protease of approximately 40 kDa produced in the kidney by juxtaglomerular cells of the macula densa.{12331} Renin converts angiotensinogen into angiotensin I, activating a major arm of renal sodium and volume auto-regulation. Native human renin is a glycoprotein derived from preprorenin by sequential cleavage. The first cleavage releases the signal peptide (amino acids 1-23), forming the prorenin (44 kDa). The second cleavage removes the propeptide (amino acids 24-66), forming the functional renin protein (40 kDa). This antibody detects both the active renin and prorenin.  

     

    Brand:
    Cayman
    SKU:17624 - 200 µg

    Available on backorder

  • Resistin is a peptide hormone belonging to the class of cysteine-rich secreted proteins termed the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Resistin impairs glucose tolerance and insulin action in mice and also inhibits adipogenesis of murine 3T3-L1 cells. Therefore, resistin has been proposed as an adipocyte secreted factor linking obesity and type 2 diabetes. This Enzyme Immunometric Assay (EIA) is based on a double-antibody sandwich technique for quantification of human resistin. [Bertin Catalog No. A05177]  

     

    Brand:
    Cayman
    SKU:10007610 - 96 wells

    Available on backorder

  • Resistin is a peptide hormone belonging to the class of cysteine-rich secreted proteins termed the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Resistin impairs glucose tolerance and insulin action in mice and also inhibits adipogenesis of murine 3T3-L1 cells. Therefore, resistin has been proposed as an adipocyte secreted factor linking obesity and type 2 diabetes. This Enzyme Immunometric Assay (EIA) is based on a double-antibody sandwich technique for quantification of rat resistin. [Bertin Catalog No. A05179]  

     

    Brand:
    Cayman
    SKU:10007612 - 96 wells

    Available on backorder

  • Brand:
    Cayman
    SKU:400382 - 100 dtn

    Available on backorder

  • Brand:
    Cayman
    SKU:400382 - 500 dtn

    Available on backorder

  • Cayman’s Resolvin D1 (RvD1) ELISA Kit is a competitive assay that can be used for quantification of RvD1 (17(S)-Resolvin D1) in plasma, serum, and cell culture supernatants. The assay has a range from 3.3-2,000 pg/ml and a sensitivity (80% B/B0) of approximately 15 pg/ml.  

     

    Brand:
    Cayman
    SKU:500380 - 480 solid wells

    Available on backorder

  • Cayman’s Resolvin D1 (RvD1) ELISA Kit is a competitive assay that can be used for quantification of RvD1 (17(S)-Resolvin D1) in plasma, serum, and cell culture supernatants. The assay has a range from 3.3-2,000 pg/ml and a sensitivity (80% B/B0) of approximately 15 pg/ml.  

     

    Brand:
    Cayman
    SKU:500380 - 480 strip wells

    Available on backorder

  • Cayman’s Resolvin D1 (RvD1) ELISA Kit is a competitive assay that can be used for quantification of RvD1 (17(S)-Resolvin D1) in plasma, serum, and cell culture supernatants. The assay has a range from 3.3-2,000 pg/ml and a sensitivity (80% B/B0) of approximately 15 pg/ml.  

     

    Brand:
    Cayman
    SKU:500380 - 96 solid wells

    Available on backorder

  • Cayman’s Resolvin D1 (RvD1) ELISA Kit is a competitive assay that can be used for quantification of RvD1 (17(S)-Resolvin D1) in plasma, serum, and cell culture supernatants. The assay has a range from 3.3-2,000 pg/ml and a sensitivity (80% B/B0) of approximately 15 pg/ml.  

     

    Brand:
    Cayman
    SKU:500380 - 96 strip wells

    Available on backorder

  • Brand:
    Cayman
    SKU:400384 - 100 ng

    Available on backorder

  • Brand:
    Cayman
    SKU:401122 - 100 dtn

    Available on backorder

  • Brand:
    Cayman
    SKU:401122 - 500 dtn

    Available on backorder

  • Resolvin D2 (RvD2) is produced physiologically from the sequential oxygenation of docosahexaenoic acid (DHA) by 15- and 5-lipoxygenases. RvD2 reduces polymorphonuclear leukocyte transendothelial migration, the earliest event in acute inflammation, by ~70% at doses as low as 10 pg in the mouse model of peritonitis. In the rat model of burn injury and sepsis, RvD2 restores defective neutrophil motility and dramatically increases survival at doses of 8-10 ng/rat. Resolvins also enhance bacterial clearance by phagocytosis and have been shown to synergistically enhance certain antibiotics. Cayman’s Resolvin D2 ELISA Kit is a competitive assay that can be used for quantification of RvD2. Due to the number and variation of potential sample types, this assay has been validated in Cayman’s ELISA Buffer (Item No. 400060) diluted to 1X (0.1 M potassium phosphate containing 0.1% BSA, 400 mM sodium chloride, 1 mM EDTA, and 0.01% sodium azide), human plasma, and human serum. The assay has a range from 1.6-1,000 pg/ml and a sensitivity (80% B/B0) of approximately 10 pg/ml.  

     

    Brand:
    Cayman
    SKU:501120 - 480 solid wells

    Available on backorder

  • Resolvin D2 (RvD2) is produced physiologically from the sequential oxygenation of docosahexaenoic acid (DHA) by 15- and 5-lipoxygenases. RvD2 reduces polymorphonuclear leukocyte transendothelial migration, the earliest event in acute inflammation, by ~70% at doses as low as 10 pg in the mouse model of peritonitis. In the rat model of burn injury and sepsis, RvD2 restores defective neutrophil motility and dramatically increases survival at doses of 8-10 ng/rat. Resolvins also enhance bacterial clearance by phagocytosis and have been shown to synergistically enhance certain antibiotics. Cayman’s Resolvin D2 ELISA Kit is a competitive assay that can be used for quantification of RvD2. Due to the number and variation of potential sample types, this assay has been validated in Cayman’s ELISA Buffer (Item No. 400060) diluted to 1X (0.1 M potassium phosphate containing 0.1% BSA, 400 mM sodium chloride, 1 mM EDTA, and 0.01% sodium azide), human plasma, and human serum. The assay has a range from 1.6-1,000 pg/ml and a sensitivity (80% B/B0) of approximately 10 pg/ml.  

     

    Brand:
    Cayman
    SKU:501120 - 480 strip wells

    Available on backorder

  • Resolvin D2 (RvD2) is produced physiologically from the sequential oxygenation of docosahexaenoic acid (DHA) by 15- and 5-lipoxygenases. RvD2 reduces polymorphonuclear leukocyte transendothelial migration, the earliest event in acute inflammation, by ~70% at doses as low as 10 pg in the mouse model of peritonitis. In the rat model of burn injury and sepsis, RvD2 restores defective neutrophil motility and dramatically increases survival at doses of 8-10 ng/rat. Resolvins also enhance bacterial clearance by phagocytosis and have been shown to synergistically enhance certain antibiotics. Cayman’s Resolvin D2 ELISA Kit is a competitive assay that can be used for quantification of RvD2. Due to the number and variation of potential sample types, this assay has been validated in Cayman’s ELISA Buffer (Item No. 400060) diluted to 1X (0.1 M potassium phosphate containing 0.1% BSA, 400 mM sodium chloride, 1 mM EDTA, and 0.01% sodium azide), human plasma, and human serum. The assay has a range from 1.6-1,000 pg/ml and a sensitivity (80% B/B0) of approximately 10 pg/ml.  

     

    Brand:
    Cayman
    SKU:501120 - 96 solid wells

    Available on backorder

  • Resolvin D2 (RvD2) is produced physiologically from the sequential oxygenation of docosahexaenoic acid (DHA) by 15- and 5-lipoxygenases. RvD2 reduces polymorphonuclear leukocyte transendothelial migration, the earliest event in acute inflammation, by ~70% at doses as low as 10 pg in the mouse model of peritonitis. In the rat model of burn injury and sepsis, RvD2 restores defective neutrophil motility and dramatically increases survival at doses of 8-10 ng/rat. Resolvins also enhance bacterial clearance by phagocytosis and have been shown to synergistically enhance certain antibiotics. Cayman’s Resolvin D2 ELISA Kit is a competitive assay that can be used for quantification of RvD2. Due to the number and variation of potential sample types, this assay has been validated in Cayman’s ELISA Buffer (Item No. 400060) diluted to 1X (0.1 M potassium phosphate containing 0.1% BSA, 400 mM sodium chloride, 1 mM EDTA, and 0.01% sodium azide), human plasma, and human serum. The assay has a range from 1.6-1,000 pg/ml and a sensitivity (80% B/B0) of approximately 10 pg/ml.  

     

    Brand:
    Cayman
    SKU:501120 - 96 strip wells

    Available on backorder

  • Brand:
    Cayman
    SKU:401124 - 50 ng

    Available on backorder

  • The p90 ribosomal S6 kinases (RSKs) 1-4 are downstream members of the extracellular signal-regulated kinase (ERK)/MAPK cascade. The loss of RSK2 activity in humans leads to Coffin-Lowry syndrome, which is characterized by mental retardation and growth deficits.{14437} Recent work suggests that RSK2 exerts a tonic regulation on G protein-coupled signaling.{14438}  

     

    Brand:
    Cayman
    SKU:10009411 - 1 ea

    Available on backorder

  • Apoptosis is mediated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. The DD-containing serine/threonine kinase RIP regulates Fas-induced apoptosis. A novel CARD-containing serine/threonine kinase that regulates apoptosis was identified and designated RICK for RIP-like interacting CLARP kinase.{7167} RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain. The RICK kinase domain has high sequence homology to that of RIP. Overexpression of RICK promotes the activation of caspase-8 and Fas-induced apoptosis. RICK represents a novel kinase that regulates Fas-induced apoptosis. The mRNA for RICK is expressed in multiple human tissues.{7167} RICK interacts with several Nod and T-cell receptor proteins and thereby confers signal transduction for innate and adaptive immune system responses.{12843}  

     

    Brand:
    Cayman
    SKU:160785 - 500 µl

    Available on backorder

  • Retinoic acid-inducible gene I (RIG-I), also known as DDX58, is a cytosolic DExD/H-box RNA helicase and an immune sensing receptor encoded by DDX58 in humans.{48287} It is composed of a C-terminal repressor domain, a central RNA helicase domain, and two N-terminal caspase recruitment domains (CARDs).{48287,48288} Following recognition of viral dsRNA by the C-terminal and helicase domains, the helicase domain induces an ATP-dependent conformational change, allowing for interaction of the CARD domains with mitochondrial antiviral-signaling protein (MAVS) and induction of a type I interferon (IFN) response.{48287,48288,48289} RIG-I levels are elevated in the epidermis of patients with psoriasis.{48288} DDX58 expression is decreased in tumor tissues from patients with hepatocellular carcinoma (HCC) compared to nontumor hepatic tissue, and low DDX58 expression is associated with poor disease prognosis.{46559} Cayman’s RIG-I Monoclonal Antibody (Clone 1E3) can be used for Western blot, ELISA, and immunohistochemistry applications. The antibody recognizes RIG-I at 106.6 kDa from human and mouse samples.  

     

    Brand:
    Cayman
    SKU:25300 - 100 µg

    Available on backorder

  • [Bertin Catalog No. G01033]  

     

    Brand:
    Cayman
    SKU:32823 - 100 µl

    Available on backorder

  • RORγ is a 560 amino acid novel DNA-binding transcription factor belonging to the ROR/RZR orphan nuclear receptor subfamily. The specific functions of this protein are not known. However, reports suggest that this protein is a ligand activated transcription factor and may be essential for lymphoid organogenesis (lymph nodes and Peyer’s patches) and may play an important regulatory role in controlling apoptosis during thymopoiesis. RORγ inhibits the expression of Fas ligand and IL2 and directs the differentiation program of proinflammatory IL-17+ T helper cells. ROR-γ shares 50 to 51% sequence identity with RORα and RORβ and is 88% identical to mouse RORγ. It is highly expressed in skeletal muscle and at lower levels in various other tissues.  

     

    Brand:
    Cayman
    SKU:11003 - 1 ea

    Available on backorder

  • 2,7-Dichlorofluoroscin Diacetate (DCFDA or 2,7-dichlorodihydrofluoroscein diacetate) is a widely-used, cell-permeable redox sensitive fluorescent probe that is oxidized by ROS and certain RNS to yield the highly fluorescent product 2,7-dichlorofluoroscein. Cayman’s ROS Detection Cell-Based Assay Kit (DCFDA) uses DCFDA as a fluorescent probe for the detection of ROS generation. Pyocyanin, a cell-permeable compound capable of redox cycling, is included as a positive control for ROS generation. N-acetyl-cysteine is included as an antioxidant control.  

     

    Brand:
    Cayman
    SKU:601520 - 96 wells

    Available on backorder

  • Dihydroethidium (hydroethidine or DHE) is a widely used ethidium-based, redox-sensitive fluorescent probe. DHE has been shown to be oxidized by superoxide to form 2-hydroxyethidium (2-OH-E+) (ex 500-530 nm/em 590-620 nm) or by non-specific oxidation by other sources of reactive oxygen species (ROS) to form ethidium (E+) (ex 480 nm/em 576 nm).{32281,32282} Given their narrow spectral range, distinguishing between the two species using filter-based optical systems is often difficult and can lead to misreporting of the species of ROS being generated. This assay kit uses DHE as a fluorescent probe for the detection of ROS generation. Antimycin A, an inhibitor of complex III of the mitochondrial electron transport chain, is included as a positive control for ROS generation. N-acetyl Cysteine is included as an antioxidant control.  

     

    Brand:
    Cayman
    SKU:601290 - 96 wells

    Available on backorder

  • p90 ribosomal S6 kinase 1 (RSK1) is a member of the RSK family of serine/threonine kinases that mediates RAS/RAF/MEK/ERK/MAPK signaling.{57305} It contains N- and C-terminal kinase domains connected by a linker region, which contains various residues, including threonine 359 (Thr359) and serine 363 (Ser363), that are subject to phosphorylation and lead to its activation. RSK1 is ubiquitously expressed and localized to the cytoplasm, where it is in a complex with its activator ERK1/2 in quiescent cells.{57305,24631} Upon stimulation of receptor tyrosine kinases (RTKs) by a variety of cytokines, neurotransmitters, or hormones, RSK1 accumulates at the plasma membrane and is phosphorylated by ERK1/2 and 3-phosphoinositide-dependent protein kinase 1 (PDK1), resulting in dissociation of the ERK1/2-RSK1 complex and activation of RSK1.{57304,57305,24631} RSK1 phosphorylates a variety of substrates that have roles in several cellular processes, including cell growth, survival, and proliferation.{24631} Tumor RSK1 levels are increased in patients with breast or prostate cancer.{57305} Cayman’s RSK1 (Phospho-Thr359/Ser363) Monoclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications.  

     

    Brand:
    Cayman
    SKU:32193 - 100 µl

    Available on backorder

  • Brand:
    Cayman
    SKU:32824 - 100 µl

    Available on backorder

  • [Bertin Catalog No. G01036]  

     

    Brand:
    Cayman
    SKU:32826 - 100 µl

    Available on backorder

  • [Bertin Catalog No. G01037]  

     

    Brand:
    Cayman
    SKU:32827 - 100 µl

    Available on backorder

  • Sphingosine-1-phosphate (S1P) exerts its activity by binding to five distinct G-protein-coupled receptors, S1P1/EDG-1, S1P2/EDG-5, S1P3/EDG-3, S1P4/EDG-6, and S1P5/EDG-8.{11895,12477} S1P1 primarily couples with pertussis toxin-sensitive Gi/o proteins to mediate S1P-induced cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis.{11895,12477,10628} Expression of S1P1 is abundant in embryological vasculature and is ubiquitously expressed in adult cells suggesting diverse physiological functions of this receptor.{12477} The human and mouse S1P1 receptors have 382 amino acids with an estimated molecular weight of 43 kDa. Glycosylation at the N-terminal extracellular domain may cause the protein to migrate at a higher position in SDS-PAGE.{12476} Cayman’s S1P1 Polyclonal Antibody detects the receptor at 47 kDa by WB analysis. The antibody can also be used for IF and IHC to study expression patterns of this protein.  

     

    Brand:
    Cayman
    SKU:10005228 - 1 ea

    Available on backorder

  • Sphingosine-1-phosphate (S1P) exerts its activity by binding to five distinct G protein-coupled receptors, S1P1/EDG-1, S1P2/EDG-5, S1P3/EDG-3, S1P4/EDG-6, and S1P5/EDG-8.{11895,12477} S1P1 primarily couples with pertussis toxin-sensitive Gi/o proteins to mediate S1P-induced cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis.{11895,12477,10628} Expression of S1P1 is abundant in embryological vasculature and is ubiquitously expressed in adult cells, suggesting diverse physiological functions of this receptor.{12477} The human and mouse S1P1 receptors have 382 amino acids with an estimated molecular weight of 43 kDa. Glycosylation at the N-terminal extracellular domain may cause the protein to migrate at a higher position in SDS-PAGE.{12476} Cayman’s S1P1 Polyclonal Antibody (protein A-purified) can be used for western blot applications. The antibody recognizes S1P1 at 42 kDa from human, mouse, porcine, and rat samples.  

     

    Brand:
    Cayman
    SKU:20208 - 1 ea

    Available on backorder

  • Sphingosine-1-phosphate (S1P) exerts its activity by binding to five distinct G-protein-coupled receptors, S1P1/EDG-1, S1P2/EDG-5, S1P3/EDG-3, S1P4/EDG-6, and S1P5/EDG-8.{11895,12477} S1P3 couples to Gi/o-ERK, Gq-PLC, and G12/13-Rho axes to mediate S1P-induced cell proliferation, survival, migration, and related signaling events.{11895,12477,10628} S1P3 is widely expressed in various tissues, suggesting diverse physiological functions of this receptor.{10623} The human and mouse S1P3 have 378 amino acids with an estimated molecular weight of 42 kDa. Glycosylation at the N-terminal extracellular domain may cause the protein to migrate at different positions in SDS-PAGE. Cayman’s S1P3 Polyclonal Antibody can be used for immunocytochemistry and Western blot applications. The antibody recognizes S1P3 at 47 kDa from human, mouse, and rat samples. Liver and heart tissues display 2-3 bands between 40-50 kDa, possibly due to different degree of glycosylation.  

     

    Brand:
    Cayman
    SKU:10006373 - 1 ea

    Available on backorder

  • EDG-6 is a member of the endothelial differentiation gene (EDG) G protein-coupled receptor family and is a receptor for sphingosine 1-phosphate (S1P). Binding of S1P to EDG-6 activates Erk, leading to the activation of the transcription factor Elk1 and thus the mitogen-activated protein kinase (MAPK) signal transduction pathway. The protein is specifically expressed in fetal and adult lymphoid and hematopoietic tissue as well as in lung. Human EDG-6 gene is intronless and is mapped to chromosome 19p13.3 at the D19S120 marker.  

     

    Brand:
    Cayman
    SKU:13489 - 1 ea

    Available on backorder

  • Sphingosine 1-phosphate (S1P) receptor EDG-8 is a multi-pass membrane protein belonging to the G protein-coupled receptor 1 family and coupled to both the Gi/0a and G12 subclass of heteromeric G-proteins. EDG-8 acts as a receptor for the bioactive sphingolipid S1P as well as for lysophosphatidic acid (LPA) and gives diverse physiological effects on most types of cells and tissues. EDG-8 may also play a regulatory role in the transformation of radial glial cells into astrocytes and affect proliferative activity of these cells. It is widely expressed in white matter tracts of brain, spleen, peripheral blood leukocytes, placenta, lung, aorta, and fetal spleen. Over expression of EDG-8 leads to a lymphoproliferative disorder often associated with the autoimmune disease known as leukemic large granular lymphocyte (LGL).  

     

    Brand:
    Cayman
    SKU:13490 - 1 ea

    Available on backorder

  • Brand:
    Cayman
    SKU:601011 - 50 µl

    Available on backorder

  • Brand:
    Cayman
    SKU:600491 - 10 ml

    Available on backorder

  • Brand:
    Cayman
    SKU:600491 - 2 ml

    Available on backorder

  • Severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid protein is a viral protein encoded by the N gene in SARS-CoV RNA.{53462} SARS-CoV is a member of the Betacoronavirus genus of viruses and has an approximately 79% sequence identity with SARS-CoV-2, the causative agent of COVID-19.{53459,53461} The SARS-CoV nucleocapsid protein has a greater than 90% similarity to the SARS-CoV-2 nucleocapsid protein, and SARS-CoV-2 contains 27 T cell epitopes that are identical to SARS-CoV T cell epitopes.{49555} The SARS-CoV nucleocapsid protein packages the viral RNA into a helical ribonucleoprotein complex (RNP) that is a template for viral replication.{53460} It is integral for viral self-assembly and involved with regulation of the cell cycle. SARS-CoV is the causative agent of SARS, a primarily respiratory illness characterized by fever, cough, shortness of breath, and an approximately 10% fatality rate.{53461} Cayman’s SARS-CoV Nucleocapsid Protein Polyclonal Antibody can be used for ELISA.  

     

    Brand:
    Cayman
    SKU:31430 - 100 µl

    Available on backorder

  • Severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid protein is a viral protein encoded by the N gene in SARS-CoV RNA.{53462} SARS-CoV is a member of the Betacoronavirus genus of viruses and has an approximately 79% sequence identity with SARS-CoV-2, the causative agent of COVID-19.{53459,53461} The SARS-CoV nucleocapsid protein has a greater than 90% similarity to the SARS-CoV-2 nucleocapsid protein, and SARS-CoV-2 contains 27 T cell epitopes that are identical to SARS-CoV T cell epitopes.{49555} The SARS-CoV nucleocapsid protein packages the viral RNA into a helical ribonucleoprotein complex (RNP) that is a template for viral replication.{53460} It is integral for viral self-assembly and involved with regulation of the cell cycle. SARS-CoV is the causative agent of SARS, a primarily respiratory illness characterized by fever, cough, shortness of breath, and an approximately 10% fatality rate.{53461} Cayman’s SARS-CoV Nucleocapsid Protein Polyclonal Antibody can be used for ELISA.  

     

    Brand:
    Cayman
    SKU:31430 - 200 µl

    Available on backorder

  • Severe acute respiratory syndrome coronavirus (SARS-CoV) surface glycoprotein, also known as the spike glycoprotein, is encoded by the S gene in SARS-CoV RNA.{53462} SARS-CoV is a member of the Betacoronavirus genus of viruses and has an approximately 79% sequence identity with SARS-CoV-2, the causative agent of COVID-19.{53459,53461} The spike protein of SARS-CoV and the related viruses SARS-CoV-2 and Middle East respiratory syndrome coronavirus (MERS-CoV) is a transmembrane glycoprotein that assembles into homotrimers on the virus surface and is comprised of an N-terminal S1 subunit, which contains the receptor binding domain (RBD), and a C-terminal S2 subunit, which facilitates fusion between viral and host cell membranes.{46767,46768,49561} The 193-amino acid RBD of the SARS-CoV spike protein is a target for neutralizing antibodies.{46768,49525} The SARS-CoV RBD, which spans amino acid residues 318 to 510, is 73% identical to that of SARS-CoV-2 and can bind to human angiotensin-converting enzyme 2 (ACE2), which is the host cell surface receptor for both SARS-CoV and SARS-CoV-2.{46767,46768,49561,49525} SARS-CoV is the causative agent of SARS, a primarily respiratory illness characterized by fever, cough, shortness of breath, and an approximately 10% fatality rate.{53461} Cayman’s SARS-CoV Surface Glycoprotein S1 Subunit Monoclonal Antibody can be used for ELISA. The antibody recognizes the S1 subunit to detect the SARS-CoV surface glycoprotein.  

     

    Brand:
    Cayman
    SKU:31431 - 100 µl

    Available on backorder

  • Severe acute respiratory syndrome coronavirus (SARS-CoV) surface glycoprotein, also known as the spike glycoprotein, is encoded by the S gene in SARS-CoV RNA.{53462} SARS-CoV is a member of the Betacoronavirus genus of viruses and has an approximately 79% sequence identity with SARS-CoV-2, the causative agent of COVID-19.{53459,53461} The spike protein of SARS-CoV and the related viruses SARS-CoV-2 and Middle East respiratory syndrome coronavirus (MERS-CoV) is a transmembrane glycoprotein that assembles into homotrimers on the virus surface and is comprised of an N-terminal S1 subunit, which contains the receptor binding domain (RBD), and a C-terminal S2 subunit, which facilitates fusion between viral and host cell membranes.{46767,46768,49561} The 193-amino acid RBD of the SARS-CoV spike protein is a target for neutralizing antibodies.{46768,49525} The SARS-CoV RBD, which spans amino acid residues 318 to 510, is 73% identical to that of SARS-CoV-2 and can bind to human angiotensin-converting enzyme 2 (ACE2), which is the host cell surface receptor for both SARS-CoV and SARS-CoV-2.{46767,46768,49561,49525} SARS-CoV is the causative agent of SARS, a primarily respiratory illness characterized by fever, cough, shortness of breath, and an approximately 10% fatality rate.{53461} Cayman’s SARS-CoV Surface Glycoprotein S1 Subunit Monoclonal Antibody can be used for ELISA. The antibody recognizes the S1 subunit to detect the SARS-CoV surface glycoprotein.  

     

    Brand:
    Cayman
    SKU:31431 - 50 µl

    Available on backorder

  • Severe acute respiratory syndrome coronavirus (SARS-CoV) surface glycoprotein, also known as the spike glycoprotein, is encoded by the S gene in SARS-CoV RNA.{53462} SARS-CoV is a member of the Betacoronavirus genus of viruses and has an approximately 79% sequence identity with SARS-CoV-2, the causative agent of COVID-19.{53459,53461} The spike protein of SARS-CoV and the related viruses SARS-CoV-2 and Middle East respiratory syndrome coronavirus (MERS-CoV) is a transmembrane glycoprotein that assembles into homotrimers on the virus surface and is comprised of an N-terminal S1 subunit, which contains the receptor binding domain (RBD), and a C-terminal S2 subunit, which facilitates fusion between viral and host cell membranes.{46767,46768,49561} The 193-amino acid RBD of the SARS-CoV spike protein is a target for neutralizing antibodies.{46768,49525} The SARS-CoV RBD, which spans amino acid residues 318 to 510, is 73% identical to that of SARS-CoV-2 and can bind to human angiotensin-converting enzyme 2 (ACE2), which is the host cell surface receptor for both SARS-CoV and SARS-CoV-2.{46768,46767,49525,49561} SARS-CoV is the causative agent of SARS, a primarily respiratory illness characterized by fever, cough, shortness of breath, and an approximately 10% fatality rate.{53461} Cayman’s SARS-CoV Surface Glycoprotein S1 Subunit Polyclonal Antibody can be used for ELISA and Western blot (WB; reducing conditions) applications.  

     

    Brand:
    Cayman
    SKU:31433 - 100 µl

    Available on backorder

  • Severe acute respiratory syndrome coronavirus (SARS-CoV) surface glycoprotein, also known as the spike glycoprotein, is encoded by the S gene in SARS-CoV RNA.{53462} SARS-CoV is a member of the Betacoronavirus genus of viruses and has an approximately 79% sequence identity with SARS-CoV-2, the causative agent of COVID-19.{53459,53461} The spike protein of SARS-CoV and the related viruses SARS-CoV-2 and Middle East respiratory syndrome coronavirus (MERS-CoV) is a transmembrane glycoprotein that assembles into homotrimers on the virus surface and is comprised of an N-terminal S1 subunit, which contains the receptor binding domain (RBD), and a C-terminal S2 subunit, which facilitates fusion between viral and host cell membranes.{46767,46768,49561} The 193-amino acid RBD of the SARS-CoV spike protein is a target for neutralizing antibodies.{46768,49525} The SARS-CoV RBD, which spans amino acid residues 318 to 510, is 73% identical to that of SARS-CoV-2 and can bind to human angiotensin-converting enzyme 2 (ACE2), which is the host cell surface receptor for both SARS-CoV and SARS-CoV-2.{46768,46767,49525,49561} SARS-CoV is the causative agent of SARS, a primarily respiratory illness characterized by fever, cough, shortness of breath, and an approximately 10% fatality rate.{53461} Cayman’s SARS-CoV Surface Glycoprotein S1 Subunit Polyclonal Antibody can be used for ELISA and Western blot (WB; reducing conditions) applications.  

     

    Brand:
    Cayman
    SKU:31433 - 200 µl

    Available on backorder

  • Severe acute respiratory syndrome coronavirus (SARS-CoV) surface glycoprotein, also known as the spike glycoprotein, is encoded by the S gene in SARS-CoV RNA.{53462} SARS-CoV is a member of the Betacoronavirus genus of viruses and has an approximately 79% sequence identity with SARS-CoV-2, the causative agent of COVID-19.{53459,53461} The spike protein of SARS-CoV and the related viruses SARS-CoV-2 and Middle East respiratory syndrome coronavirus (MERS-CoV) is a transmembrane glycoprotein that assembles into homotrimers on the virus surface and is comprised of an N-terminal S1 subunit, which contains the receptor binding domain (RBD), and a C-terminal S2 subunit, which facilitates fusion between viral and host cell membranes.{46767,46768,49561} The 193-amino acid RBD of the SARS-CoV spike protein is a target for neutralizing antibodies.{46768,49525} The SARS-CoV RBD, which spans amino acid residues 318 to 510, is 73% identical to that of SARS-CoV-2 and can bind to human angiotensin-converting enzyme 2 (ACE2), which is the host cell surface receptor for both SARS-CoV and SARS-CoV-2.{46768,46767,49525,49561} SARS-CoV is the causative agent of SARS, a primarily respiratory illness characterized by fever, cough, shortness of breath, and an approximately 10% fatality rate.{53461} Cayman’s SARS-CoV Surface Glycoprotein S1 Subunit Polyclonal Antibody can be used for ELISA and Western blot (WB; reducing conditions) applications.  

     

    Brand:
    Cayman
    SKU:31433 - 50 µl

    Available on backorder

  • SARS-CoV-2 N Antibody Pair 2 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). An antibody specific for SARS-CoV-2 Nucleoprotein (N) has been pre-coated onto the microwells.

    Brand:
    Cusabio
    SKU:CSB-EAP33255A2

    Available on backorder

  • Cayman’s SARS-CoV-2 Neutralizing Antibody Human Plasma Control Set contains human plasma collected prior to the SARS-CoV-2 pandemic that has been spiked at low, mid, or high levels with a recombinant human anti-SARS-CoV-2 neutralizing IgG antibody. A negative control, free of neutralizing antibody, is also included. The levels of neutralizing antibody to be added to each control were determined by screening numerous positive and negative samples using Cayman’s SARS-CoV-2 Neutralizing Antibody Detection ELISA Kit (Item No. 502070). The amount of neutralizing antibody spiked into the controls was designed to be representative of the range of antibody levels found in a typical sample set.  

     

    Brand:
    Cayman
    SKU:502080 - 1 ea

    Available on backorder

  • Cayman’s SARS-CoV-2 Neutralizing Antibody Human Serum Control Set contains human serum collected prior to the SARS-CoV-2 pandemic that has been spiked at low, mid, or high levels with a recombinant human anti-SARS-CoV-2 neutralizing IgG antibody. A negative control, free of neutralizing antibody, is also included. The levels of neutralizing antibody to be added to each control were determined by screening numerous positive and negative samples using Cayman’s SARS-CoV-2 Neutralizing Antibody Detection ELISA Kit (Item No. 502070). The amount of neutralizing antibody spiked into the controls was designed to be representative of the range of antibody levels found in a typical sample set.  

     

    Brand:
    Cayman
    SKU:502090 - 1 ea

    Available on backorder

  • SARS-CoV-2 Neutralizing Antibody Negative Control has been tested and formulated to work exclusively with Cayman’s SARS-CoV-2 Neutralizing Antibody Detection ELISA Kit (Item No. 502070). Please visit SARS-CoV-2 Neutralizing Antibody Detection ELISA Kit (Item No. 502070) for the kit protocol, procedures, and product handling.  

     

    Brand:
    Cayman
    SKU:502075 - 500 µl

    Available on backorder

  • SARS-CoV-2 Neutralizing Antibody Positive Control has been tested and formulated to work exclusively with Cayman’s SARS-CoV-2 Neutralizing Antibody Detection ELISA Kit (Item No. 502070). Please visit SARS-CoV-2 Neutralizing Antibody Detection ELISA Kit (Item No. 502070) for the kit protocol, procedures, and product handling.  

     

    Brand:
    Cayman
    SKU:502073 - 500 µl

    Available on backorder

  • SARS-CoV-2 Neutralizing Antibody Standard has been tested and formulated to work exclusively with Cayman’s SARS-CoV-2 Neutralizing Antibody Detection ELISA Kit (Item No. 502070). Please visit SARS-CoV-2 Neutralizing Antibody Detection ELISA Kit (Item No. 502070) for the kit protocol, procedures, and product handling.  

     

    Brand:
    Cayman
    SKU:502074 - 1 µg

    Available on backorder

  • Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive-stranded RNA virus and the causative agent of COVID-19, a primarily respiratory illness characterized by fever, cough, and shortness of breath that can lead to life-threatening complications.{53462,53459,53461,55257,55258} The SARS-CoV-2 genome encodes four structural proteins: surface glycoprotein, envelope, membrane, and nucleocapsid.{53459,53462} The surface glycoprotein, also known as the spike glycoprotein, is located on the outer envelope of the virion and binds to the carboxypeptidase angiotensin-converting enzyme 2 (ACE2), which acts as the functional receptor for SARS-CoV-2 and facilitates virus cell entry.{53462} SARS-CoV-2 infection results in the production of neutralizing antibodies, which bind to the SARS-CoV-2 spike glycoprotein receptor binding domain and prevent further viral entry and infection, starting approximately 4-10 days after symptom onset.{55337,55338} Plasma levels of SARS-CoV-2 spike glycoprotein-specific IgG antibodies increase for at least four weeks following symptom onset.{55337,55339} SARS-CoV-2 plasma antibody levels begin to decrease 2-3 months post-infection in both symptomatic and asymptomatic individuals, disappearing completely in some asymptomatic individuals.{55340} This product contains human serum negative for SARS-CoV-2 neutralizing antibodies.  

     

    Brand:
    Cayman
    SKU:31567 - 50 µl

    Available on backorder

  • Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive-stranded RNA virus and the causative agent of COVID-19, a primarily respiratory illness characterized by fever, cough, and shortness of breath that can lead to life-threatening complications.{53462,53459,53461,55257,55258} The SARS-CoV-2 genome encodes four structural proteins: surface glycoprotein, envelope, membrane, and nucleocapsid.{53459,53462} The surface glycoprotein, also known as the spike glycoprotein, is located on the outer envelope of the virion and binds to the carboxypeptidase angiotensin-converting enzyme 2 (ACE2), which acts as the functional receptor for SARS-CoV-2 and facilitates virus cell entry.{53462} SARS-CoV-2 infection results in the production of neutralizing antibodies, which bind to the SARS-CoV-2 spike glycoprotein receptor binding domain and prevent further viral entry and infection, starting approximately 4-10 days after symptom onset.{55337,55338} Plasma levels of SARS-CoV-2 spike glycoprotein-specific IgG antibodies increase for at least four weeks following symptom onset.{55337,55339} SARS-CoV-2 plasma antibody levels begin to decrease 2-3 months post-infection in both symptomatic and asymptomatic individuals, disappearing completely in some asymptomatic individuals.{55340} This product contains pre-pandemic human serum negative for SARS-CoV-2 neutralizing antibodies.  

     

    Brand:
    Cayman
    SKU:31569 - 50 µl

    Available on backorder

  • Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive-stranded RNA virus and the causative agent of COVID-19, a primarily respiratory illness characterized by fever, cough, and shortness of breath that can lead to life-threatening complications.{53462,53459,53461,55257,55258} The SARS-CoV-2 genome encodes four structural proteins: surface glycoprotein, envelope, membrane, and nucleocapsid.{53459,53462} The surface glycoprotein, also known as the spike glycoprotein, is located on the outer envelope of the virion and binds to the carboxypeptidase angiotensin-converting enzyme 2 (ACE2), which acts as the functional receptor for SARS-CoV-2 and facilitates virus cell entry.{53462} SARS-CoV-2 infection results in the production of neutralizing antibodies, which bind to the SARS-CoV-2 spike glycoprotein receptor binding domain and prevent further viral entry and infection, starting approximately 4-10 days after symptom onset.{55337,55338} Plasma levels of SARS-CoV-2 spike glycoprotein-specific IgG antibodies increase for at least four weeks following symptom onset.{55337,55339} SARS-CoV-2 plasma antibody levels begin to decrease 2-3 months post-infection in both symptomatic and asymptomatic individuals, disappearing completely in some asymptomatic individuals.{55340} This product contains human serum positive for SARS-CoV-2 neutralizing antibodies.  

     

    Brand:
    Cayman
    SKU:31568 - 50 µl

    Available on backorder

  • The SARS-CoV-2 Spike RBD Antibody is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). An antibody specific for SARS-CoV-2 Spike RBD has been pre-coated onto the microwells. The SARS-CoV-2 Spike RBD protein in samples is captured by the coated antibody after incubation.

    Brand:
    Cusabio
    SKU:CSB-EAP33245

    Available on backorder

  • Cayman’s SARS-CoV-2 Spike-ACE2 Interaction Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of the SARS-CoV-2 spike and ACE2 interaction. The assay uses a recombinant rabbit Fc-tagged SARS-CoV-2 spike S1 RBD that binds to a plate precoated with a mouse anti-rabbit antibody. A recombinant His-tagged ACE2 protein binds the spike RBD and the complex is detected with an HRP-conjugated anti-His antibody, which is easily quantified by reading the absorbance at 450 nm. A control is included for competition of the SARS-CoV-2 spike RBD-ACE2 interaction.  

     

    Brand:
    Cayman
    SKU:502050 - 96 solid wells

    Available on backorder

  • Cayman’s SARS-CoV-2 Spike-ACE2 Interaction Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of the SARS-CoV-2 spike and ACE2 interaction. The assay uses a recombinant rabbit Fc-tagged SARS-CoV-2 spike S1 RBD that binds to a plate precoated with a mouse anti-rabbit antibody. A recombinant His-tagged ACE2 protein binds the spike RBD and the complex is detected with an HRP-conjugated anti-His antibody, which is easily quantified by reading the absorbance at 450 nm. A control is included for competition of the SARS-CoV-2 spike RBD-ACE2 interaction.  

     

    Brand:
    Cayman
    SKU:502050 - 96 strip wells

    Available on backorder

  • Severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid protein is a viral protein encoded by the N gene in SARS-CoV RNA.{53462} SARS-CoV is a member of the Betacoronavirus genus of viruses and has an approximately 79% sequence identity with SARS-CoV-2.{53459,53461} The SARS-CoV nucleocapsid protein has a greater than 90% similarity to the SARS-CoV-2 nucleocapsid protein, and SARS-CoV-2 contains 27 T cell epitopes that are identical to SARS-CoV T cell epitopes.{49555} The SARS-CoV nucleocapsid protein packages the viral RNA into a helical ribonucleoprotein complex (RNP) that is a template for viral replication.{53460} It is integral for viral self-assembly and involved with regulation of the cell cycle. SARS-CoV and SARS-CoV-2 are the causative agents of SARS and COVID-19, respectively, both of which are primarily respiratory illnesses characterized by fever, cough, and shortness of breath that can lead to life-threatening complications.{53461,55257,55258} Cayman’s SARS-CoV/SARS-CoV-2 Nucleocapsid Protein Monoclonal Antibody (Clone 001) can be used for ELISA and Western blot (WB; reducing conditions) applications.  

     

    Brand:
    Cayman
    SKU:30822 - 100 µl

    Available on backorder

  • Severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid protein is a viral protein encoded by the N gene in SARS-CoV RNA.{53462} SARS-CoV is a member of the Betacoronavirus genus of viruses and has an approximately 79% sequence identity with SARS-CoV-2.{53459,53461} The SARS-CoV nucleocapsid protein has a greater than 90% similarity to the SARS-CoV-2 nucleocapsid protein, and SARS-CoV-2 contains 27 T cell epitopes that are identical to SARS-CoV T cell epitopes.{49555} The SARS-CoV nucleocapsid protein packages the viral RNA into a helical ribonucleoprotein complex (RNP) that is a template for viral replication.{53460} It is integral for viral self-assembly and involved with regulation of the cell cycle. SARS-CoV and SARS-CoV-2 are the causative agents of SARS and COVID-19, respectively, both of which are primarily respiratory illnesses characterized by fever, cough, and shortness of breath that can lead to life-threatening complications.{53461,55257,55258} Cayman’s SARS-CoV/SARS-CoV-2 Nucleocapsid Protein Monoclonal Antibody (Clone 001) can be used for ELISA and Western blot (WB; reducing conditions) applications.  

     

    Brand:
    Cayman
    SKU:30822 - 50 µl

    Available on backorder

  • Severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid protein is a viral protein encoded by the N gene in SARS-CoV RNA.{53462} SARS-CoV is a member of the Betacoronavirus genus of viruses and has an approximately 79% sequence identity with SARS-CoV-2.{53459,53461} The SARS-CoV nucleocapsid protein has a greater than 90% similarity to the SARS-CoV-2 nucleocapsid protein, and SARS-CoV-2 contains 27 T cell epitopes that are identical to SARS-CoV T cell epitopes.{49555} The SARS-CoV nucleocapsid protein packages the viral RNA into a helical ribonucleoprotein complex (RNP) that is a template for viral replication.{53460} It is integral for viral self-assembly and involved with regulation of the cell cycle. SARS-CoV and SARS-CoV-2 are the causative agents of SARS and COVID-19, respectively, both of which are primarily respiratory illnesses characterized by fever, cough, and shortness of breath that can lead to life-threatening complications.{53461,55257,55258} Cayman’s SARS-CoV/SARS-CoV-2 Nucleocapsid Protein Monoclonal Antibody can be used for ELISA and Western blot (WB; reducing conditions) applications.  

     

    Brand:
    Cayman
    SKU:31428 - 100 µl

    Available on backorder

  • Severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid protein is a viral protein encoded by the N gene in SARS-CoV RNA.{53462} SARS-CoV is a member of the Betacoronavirus genus of viruses and has an approximately 79% sequence identity with SARS-CoV-2.{53459,53461} The SARS-CoV nucleocapsid protein has a greater than 90% similarity to the SARS-CoV-2 nucleocapsid protein, and SARS-CoV-2 contains 27 T cell epitopes that are identical to SARS-CoV T cell epitopes.{49555} The SARS-CoV nucleocapsid protein packages the viral RNA into a helical ribonucleoprotein complex (RNP) that is a template for viral replication.{53460} It is integral for viral self-assembly and involved with regulation of the cell cycle. SARS-CoV and SARS-CoV-2 are the causative agents of SARS and COVID-19, respectively, both of which are primarily respiratory illnesses characterized by fever, cough, and shortness of breath that can lead to life-threatening complications.{53461,55257,55258} Cayman’s SARS-CoV/SARS-CoV-2 Nucleocapsid Protein Monoclonal Antibody can be used for ELISA and Western blot (WB; reducing conditions) applications.  

     

    Brand:
    Cayman
    SKU:31428 - 50 µl

    Available on backorder

  • Severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid protein is a viral protein encoded by the N gene in SARS-CoV RNA.{53462} SARS-CoV is a member of the Betacoronavirus genus of viruses and has an approximately 79% sequence identity with SARS-CoV-2.{53459,53461} The SARS-CoV-2 nucleocapsid protein gene sequence is greater than 90% similar to the SARS-CoV nucleocapsid protein, and it contains 27 T cell epitopes that are identical to SARS-CoV T cell epitopes.{49555} The SARS-CoV nucleocapsid protein packages the viral RNA into a helical ribonucleoprotein complex (RNP) that is a template for viral replication.{53460} It is integral for viral self-assembly and is involved with regulation of the cell cycle. SARS-CoV and SARS-CoV-2 are the causative agents of SARS and COVID-19, respectively, both of which are primarily respiratory illnesses characterized by fever, cough, and shortness of breath that can lead to life-threatening complications.{53461,55257,55258} Cayman’s SARS-CoV/SARS-CoV-2 Nucleocapsid Protein Polyclonal Antibody can be used for ELISA and Western blot (WB) applications.  

     

    Brand:
    Cayman
    SKU:31429 - 100 µl

    Available on backorder

  • Severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid protein is a viral protein encoded by the N gene in SARS-CoV RNA.{53462} SARS-CoV is a member of the Betacoronavirus genus of viruses and has an approximately 79% sequence identity with SARS-CoV-2.{53459,53461} The SARS-CoV-2 nucleocapsid protein gene sequence is greater than 90% similar to the SARS-CoV nucleocapsid protein, and it contains 27 T cell epitopes that are identical to SARS-CoV T cell epitopes.{49555} The SARS-CoV nucleocapsid protein packages the viral RNA into a helical ribonucleoprotein complex (RNP) that is a template for viral replication.{53460} It is integral for viral self-assembly and is involved with regulation of the cell cycle. SARS-CoV and SARS-CoV-2 are the causative agents of SARS and COVID-19, respectively, both of which are primarily respiratory illnesses characterized by fever, cough, and shortness of breath that can lead to life-threatening complications.{53461,55257,55258} Cayman’s SARS-CoV/SARS-CoV-2 Nucleocapsid Protein Polyclonal Antibody can be used for ELISA and Western blot (WB) applications.  

     

    Brand:
    Cayman
    SKU:31429 - 50 µl

    Available on backorder

  • Severe acute respiratory syndrome coronavirus (SARS-CoV) surface glycoprotein, also known as the spike protein, is a viral structural protein encoded by the S gene in SARS-CoV RNA that contains the receptor binding domain (RBD).{53462} SARS-CoV is a member of the Betacoronavirus genus of viruses and has an approximately 79% sequence identity with SARS-CoV-2, the causative agent of COVID-19.{53459,53461} The spike protein of SARS-CoV and the related viruses SARS-CoV-2 and Middle East respiratory syndrome coronavirus (MERS-CoV) is a transmembrane glycoprotein that assembles into homotrimers on the virus surface and is comprised of an N-terminal S1 subunit, which contains the receptor binding domain (RBD), and a C-terminal S2 subunit, which facilitates fusion between viral and host cell membranes.{46767,46768,49561} The 193-amino acid RBD of the SARS-CoV spike protein is a target for neutralizing antibodies.{46768,49525} The SARS-CoV RBD, which spans amino acid residues 318 to 510, is 73% identical to that of SARS-CoV-2 and can bind to human angiotensin-converting enzyme 2 (ACE2), which is the host cell surface receptor for both SARS-CoV and SARS-CoV-2.{46767,46768,49561,49525} SARS-CoV is the causative agent of SARS, a primarily respiratory illness characterized by fever, cough, shortness of breath, and an approximately 10% fatality rate.{53461} Cayman’s SARS-CoV-2 Surface Glycoprotein Chimeric Monoclonal Antibody is comprised of human IgG1κ constant domains and variable regions from a mouse immunized with purified recombinant SARS-CoV Surface Glycoprotein RBD protein. This antibody can be used for ELISA.  

     

    Brand:
    Cayman
    SKU:30832 - 100 µl

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  • Severe acute respiratory syndrome coronavirus (SARS-CoV) surface glycoprotein, also known as the spike protein, is a viral structural protein encoded by the S gene in SARS-CoV RNA that contains the receptor binding domain (RBD).{53462} SARS-CoV is a member of the Betacoronavirus genus of viruses and has an approximately 79% sequence identity with SARS-CoV-2, the causative agent of COVID-19.{53459,53461} The spike protein of SARS-CoV and the related viruses SARS-CoV-2 and Middle East respiratory syndrome coronavirus (MERS-CoV) is a transmembrane glycoprotein that assembles into homotrimers on the virus surface and is comprised of an N-terminal S1 subunit, which contains the receptor binding domain (RBD), and a C-terminal S2 subunit, which facilitates fusion between viral and host cell membranes.{46767,46768,49561} The 193-amino acid RBD of the SARS-CoV spike protein is a target for neutralizing antibodies.{46768,49525} The SARS-CoV RBD, which spans amino acid residues 318 to 510, is 73% identical to that of SARS-CoV-2 and can bind to human angiotensin-converting enzyme 2 (ACE2), which is the host cell surface receptor for both SARS-CoV and SARS-CoV-2.{46767,46768,49561,49525} SARS-CoV is the causative agent of SARS, a primarily respiratory illness characterized by fever, cough, shortness of breath, and an approximately 10% fatality rate.{53461} Cayman’s SARS-CoV-2 Surface Glycoprotein Chimeric Monoclonal Antibody is comprised of human IgG1κ constant domains and variable regions from a mouse immunized with purified recombinant SARS-CoV Surface Glycoprotein RBD protein. This antibody can be used for ELISA.  

     

    Brand:
    Cayman
    SKU:30832 - 50 µl

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  • Secreted alkaline phosphatase (SEAP) is commonly used as a reporter of gene expression. Compared to other conventional intracellular reporters such as chloramphenicol acetyltransferase (CAT) and firefly luciferase, SEAP has the advantage of being secreted from transfected cells into the culture medium. Cayman’s Secreted Alkaline Phosphatase Reporter Gene Assay Kit (Luminescence) provides a simple chemiluminescence method for the sensitive quantitation of SEAP in conditioned cell culture medium from transfected cells. The assay can detect SEAP activity in the milliunits/well (mU/ml) range. The kit includes enough reagents to run three 96-well plates. The assay is easy to perform and can be completed within one hour.  

     

    Brand:
    Cayman
    SKU:600260 - 300 tests

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  • Brand:
    Cayman
    SKU:600037 - 1 ea

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  • Brand:
    Cayman
    SKU:600036 - 1 ea

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  • Detection of the egg yolk protein vitellogenin (Vtg), egg shell proteins Zona radiata proteins (Zrp), cytochrome P450 1A (CYP1A) and Metallothionein (MT) are simple and sensitive biomarkers for endocrine pollutant exposure in fish. The Enzyme-Linked Immunosorbent Assay (ELISA) method is a well established method suitable for detection of such biomarkers. The semi-quantitative Biomarker ELISA Kit is well suited for applications such as environmental and effluent monitoring and can easily be combined with standard fish tests according to OECD Guidelines for Testing of Chemicals.  

     

    Brand:
    Cayman
    SKU:10007680 - 480 wells

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  • Detection of the egg yolk protein vitellogenin (Vtg), egg shell proteins Zona radiata proteins (Zrp), cytochrome P450 1A (CYP1A) and Metallothionein (MT) are simple and sensitive biomarkers for endocrine pollutant exposure in fish. The Enzyme-Linked Immunosorbent Assay (ELISA) method is a well established method suitable for detection of such biomarkers. The semi-quantitative Biomarker ELISA Kit is well suited for applications such as environmental and effluent monitoring and can easily be combined with standard fish tests according to OECD Guidelines for Testing of Chemicals.  

     

    Brand:
    Cayman
    SKU:10007680 - 96 wells

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  • Detection of the egg yolk protein vitellogenin (Vtg), egg shell proteins Zona radiata proteins (Zrp), cytochrome P450 1A (CYP1A) and Metallothionein (MT) are simple and sensitive biomarkers for endocrine pollutant exposure in fish. The Enzyme-Linked Immunosorbent Assay (ELISA) method is a well established method suitable for detection of such biomarkers. The semi-quantitative Biomarker ELISA Kit is well suited for applications such as environmental and effluent monitoring and can easily be combined with standard fish tests according to OECD Guidelines for Testing of Chemicals.  

     

    Brand:
    Cayman
    SKU:10008659 - 480 wells

    Available on backorder

  • Detection of the egg yolk protein vitellogenin (Vtg), egg shell proteins Zona radiata proteins (Zrp), cytochrome P450 1A (CYP1A) and Metallothionein (MT) are simple and sensitive biomarkers for endocrine pollutant exposure in fish. The Enzyme-Linked Immunosorbent Assay (ELISA) method is a well established method suitable for detection of such biomarkers. The semi-quantitative Biomarker ELISA Kit is well suited for applications such as environmental and effluent monitoring and can easily be combined with standard fish tests according to OECD Guidelines for Testing of Chemicals.  

     

    Brand:
    Cayman
    SKU:10008659 - 96 wells

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  • Sphingolipids play essential roles in various cellular events, including proliferation, differentiation, senescence, apoptosis, and inflammatory responses.{12350} Serine palmitoyltransferase (SPT) is the initial and rate-limiting enzyme in the de novo sphingolipid biosynthesis, and thus regulates the level of sphingolipids in cells.{12348} Immunohistochemical study revealed widespread distribution of the enzyme with most strong expression in brain and digestive tract.{12347} Two subunits, SPT1 and SPT2 at a stoichiometry of 1:1, are involved in the enzymatic activity of SPT.{12349} Cayman Chemical’s SPT2 Polyclonal Antibody recognizes SPT2, the long chain subunit of the enzyme. The antibody stains mainly cell nuclei and occasionally both cell nuclei and cytoplasm in formalin-fixed, paraffin-embedded rat brain tissue. The nuclear localization of SPT2 may suggest that SPT2 associates with another nuclear protein or is modified and transported to the nucleus.{12348}  

     

    Brand:
    Cayman
    SKU:10005260 - 1 ea

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  • Serum retinol binding protein 4 (sRBP4) binds one equivalent of vitamin A and is one of the major retinol carriers found in the blood of mammals.{14372, 14373} Human RBP4 is a monomeric 21 kDa β-sheet-rich protein that contains three disulfide bonds and belongs to the lipocalin protein family.{14379} sRBP4 is synthesized and sequestered in hepatocytes until retinol binding triggers its secretion.{14379} In plasma, sRBP4 typically forms a 1:1 complex with the 55 kDa tetrameric protein transthyretin (TTR) which prevents RBP from being removed from the plasma by glomerular filtration.{14378} Recent studies have shown that sRBP4 is an adipocyte-derived “signal” that may contribute to the pathogenesis of type 2 diabetes.{14371, 14380} Elevation of sRBP4 causes systemic insulin resistance whereas reduction of serum concentrations improves insulin action.{14371, 14376, 14374} The highest known concentrations of this protein exist in serum, liver, and skeletal muscle.{14374,14375,149371}  

     

    Brand:
    Cayman
    SKU:10007681 - 500 µl

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  • Diverse signal transduction pathways impinging on the N-terminal tails of histones lead to a number of post-translational modifications including acetylation, phosphorylation, poly (ADP-ribosylation), ubiquitination, and methylation. These modifications play critical roles in regulating chromatin structure and gene expression.{17798} Histone methyltransferases selectively methylate evolutionarily conserved arginine or lysine residues, primarily in the N-terminal tails of histones H3 and H4. SET7/9 utilizes S-adenosylmethionine to methylate histone H3 at lysine 4.{16846,17224,17917} Human SET7/9 is a 366 amino acid protein with observed migration on SDS-PAGE at 49 kDa.  

     

    Brand:
    Cayman
    SKU:13780 - 500 µl

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  • Brand:
    Cayman
    SKU:600492 - 25 µl

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  • Brand:
    Cayman
    SKU:700271 - 1 ea

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  • Brand:
    Cayman
    SKU:700272 - 1 ea

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  • Diverse signal transduction pathways impinging on the N-terminal tails of histones lead to a number of post-translational modifications including acetylation, phosphorylation, poly(ADP-ribosylation), ubiquitination, and methylation. These modifications play critical roles in regulating chromatin structure and gene expression. Histone methyltransferases (HMTases) selectively methylate evolutionarily conserved arginine or lysine residues, primarily in the N-terminal tails of histones H3 and H4. SET7/9 is a histone specific HMTase that methylates histone H3 lysine 4. SET7/9 transfers methyl groups to lysine 4 of histone H3 in complex with S-adenosyl-L-methionine. Human SET7/9 is a 366 amino acid protein.  

     

    Brand:
    Cayman
    SKU:13731 - 1 ea

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  • Most histone lysine methyltransferases contain a conserved domain (SET) that utilizes S-adenosyl-L-methionine (SAM or AdoMet) as a co-factor to catalyze the methylation of lysine.{20237} SET7/9 (KMT7) is a SET domain-containing mono-methyltransferase that acts on a large number of histone and non-histone targets including histone H3, TAF10, p53, viral Tat, and estrogen receptor α.{16846,19351} Given this broad substrate specificity, SET7/9 has important implications for a variety of human diseases, and SET7/9 has emerged as a model for studying the catalytic mechanism SET domain histone methyltransferases. This fluorescence polarization assay is based upon a proprietary small molecule fluorescent probe* that binds to the SAM binding pocket in SET7/9. Binding of the small molecule probe to SET7/9 induces an increase in fluorescence polarization. Binding of the probe can be competed with the endogenous cofactor SAM or by the inhibitor sinefungin, but is unaffected by the histone H3 peptide substrate. The SET7/9 SAM-Screener™ Assay is robust (Z’ >0.6) and exhibits a greater than 100 mP shift over a range of 0-250 nM SET7/9. The assay is suitable for high-throughput screening in the provided 384-well plate or can be scaled to higher density plate formats (e.g., 1,536-well) if desired.*United States Patent 9,120,820  

     

    Brand:
    Cayman
    SKU:600490 - 1920 wells

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  • Most histone lysine methyltransferases contain a conserved domain (SET) that utilizes S-adenosyl-L-methionine (SAM or AdoMet) as a co-factor to catalyze the methylation of lysine.{20237} SET7/9 (KMT7) is a SET domain-containing mono-methyltransferase that acts on a large number of histone and non-histone targets including histone H3, TAF10, p53, viral Tat, and estrogen receptor α.{16846,19351} Given this broad substrate specificity, SET7/9 has important implications for a variety of human diseases, and SET7/9 has emerged as a model for studying the catalytic mechanism SET domain histone methyltransferases. This fluorescence polarization assay is based upon a proprietary small molecule fluorescent probe* that binds to the SAM binding pocket in SET7/9. Binding of the small molecule probe to SET7/9 induces an increase in fluorescence polarization. Binding of the probe can be competed with the endogenous cofactor SAM or by the inhibitor sinefungin, but is unaffected by the histone H3 peptide substrate. The SET7/9 SAM-Screener™ Assay is robust (Z’ >0.6) and exhibits a greater than 100 mP shift over a range of 0-250 nM SET7/9. The assay is suitable for high-throughput screening in the provided 384-well plate or can be scaled to higher density plate formats (e.g., 1,536-well) if desired.*United States Patent 9,120,820  

     

    Brand:
    Cayman
    SKU:600490 - 384 wells

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  • Brand:
    Cayman
    SKU:700351 - 1 ea

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  • Brand:
    Cayman
    SKU:700352 - 1 ea

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  • SET domain protein 3 (SETD3) is an actin histidine methyltransferase encoded by the SETD3 gene in humans and a member of the SET family of methyltransferases.{53440} It contains an N-terminal SET domain, responsible for transferring a methyl group from S-adenosyl methionine to histidine 73 on β-actin, that forms a cleft with a C-terminal domain that is similar to large subunit methyltransferase (LSMT) domains.{35557} SETD3 is ubiquitously expressed and localizes to the cytoplasm. SETD3 overexpression in liver cancer cell lines increases proliferation while shRNA knockdown decreases it.{53440} Overexpression of SETD3 increases tumor size in a HepG2 mouse xenograft model, and a xenograft model using cancer cells with an endogenous knockdown of SETD3 reduces tumor size. SETD3 protein levels are increased in isolated human cancer tissues compared with adjacent tissue. Setd3 knockout in mice leads to skeletal muscle myopathy, abnormal cardiac electrocardiogram, and, in female mice, delayed parturition.{35557} Cayman’s SETD3 Monoclonal Antibody can be used for ELISA and Western blot applications. The antibody recognizes SETD3 at 67 kDa from human samples.  

     

    Brand:
    Cayman
    SKU:29208 - 100 µg

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  • Brand:
    Cayman
    SKU:10010994 - 1 ea

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  • Brand:
    Cayman
    SKU:10011081 - 1 ea

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  • Brand:
    Cayman
    SKU:10010993 - 1 ea

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  • Sirtuins (SIRTs) represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs). Human SIRT1 is the homolog of yeast Silent information regulator 2 (Sir2) and has been shown to regulate the activity of the p53 tumor suppressor and inhibit apoptosis. Small molecule activators of SIRT1, such as resveratrol, extend lifespan in yeast and C. elegans in a manner that resembles caloric restriction. However, this activation of SIRT1 is under much debate. It was determined that the in vitro activation of SIRT1 and Sir2 by resveratrol was a substrate-specific event (i.e., the binding of resveratrol to SIRT1 promoted a conformational change that better accommodated the attached coumarin group in the peptide substrate). Additional research needs to be done to further elucidate the mechanism behind SIRT1 activation, as well as, to establish its role in aging, cancer, and neurodegenerative disease. Cayman’s SIRT1 Direct Fluorescent Screening Assay Kit provides a convenient fluorescence-based method for screening SIRT1 inhibitors or activators. The procedure requires only two easy steps, both performed in the same microplate. In the first step, the substrate, which comprises the p53 sequence Arg-His-Lys-Lys(e-acetyl)-AMC, is incubated with human recombinant SIRT1 along with its cosubstrate NAD+. Deacetylation sensitizes the substrate such that treatment with the developer in the second step releases a fluorescent product. The fluorophore can be easily analyzed using an excitation wavelength of 350-360 nm and an emission wavelength of 450-465 nm.  

     

    Brand:
    Cayman
    SKU:10010401 - 96 wells

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  • Brand:
    Cayman
    SKU:10011080 - 1 ea

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  • Sirtuins (SIRTs) represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs). Human SIRT1 is the homolog of yeast Sir2 (Silent information regulator 2) and has been shown to regulate the activity of the p53 tumor suppressor and inhibit apoptosis. Small molecule activators of SIRT1, such as resveratrol, extend lifespan in yeast and C. elegans in a manner that resembles caloric restriction. Cayman’s SIRT1 FRET-based Screening Assay Kit provides a convenient fluorescence-based method for screening SIRT1 inhibitors or activators. The procedure requires only two easy steps, both performed in the same microplate. In the first step, the substrate, which is coupled to the fluorophore and quencher, is incubated with human recombinant SIRT1 along with its cosubstrate NAD+. Deacetylation sensitizes the substrate such that treatment with the developer in the second step results in the separation of the quencher and fluorophore. The resulting fluorescence can be easily analyzed using an excitation wavelength of 335-345 nm and emission wavelength of 440-465 nm.  

     

    Brand:
    Cayman
    SKU:10010991 - 96 wells

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  • Brand:
    Cayman
    SKU:700282 - 1 ea

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  • Brand:
    Cayman
    SKU:700281 - 1 ea

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  • The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) that catalyze a reaction coupling lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose. Cayman’s SIRT2 Direct Fluorescent Screening Assay provides a fluorescence-based method for screening SIRT2 inhibitors or activators. The procedure requires only two easy steps, both performed in the same microplate. In the first step, the substrate, which comprises the p53 sequence Gln-Pro-Lys-Lys(ε-acetyl)-AMC, is incubated with human recombinant SIRT2 along with its cosubstrate NAD+. Deacetylation sensitizes the substrate such that treatment with the Developer in the second step releases a fluorescent product.  

     

    Brand:
    Cayman
    SKU:700280 - 96 wells

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  • Brand:
    Cayman
    SKU:10012541 - 1 ea

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  • Brand:
    Cayman
    SKU:10012540 - 1 ea

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  • The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) that catalyze a reaction coupling lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose. SIRT3 is a mitochondrial protein that plays a role in metabolic regulation and other cellular processes. It is the only human sirtuin with a direct genetic link with longevity. Additional research needs to be done to further elucidate the mechanism behind the involvement of SIRT3 in metabolic dysfunction, aging, cancer, and neurodegenerative disease. Cayman’s SIRT3 Direct Fluorescent Screening Assay Kit provides a convenient fluorescence-based method for screening SIRT3 inhibitors or activators. The procedure requires only two easy steps, both performed in the same microplate. In the first step, the substrate, which comprises the p53 sequence Gln-Pro-Lys-Lys(e-acetyl)-AMC, is incubated with human recombinant SIRT3 along with its cosubstrate NAD+. Deacetylation sensitizes the substrate such that treatment with the developer in the second step releases a fluorescent product. The fluorophore can be analyzed with an excitation wavelength of 350-360 nm and an emission wavelength of 450-465 nm.  

     

    Brand:
    Cayman
    SKU:10011566 - 96 wells

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  • SIRT7 is a member of a famiy of proteins called sirtuins (SIR2-like proteins) and are present in prokaryotes and eukaryotes. All SIR2-like proteins have a sirtuin core domain, which contains a series of sequence motifs conserved in organisms ranging from bacteria to humans. Bacterial, yeast, and mammalian sirtuins are able to metabolize NAD+ and several act as mono-ADP-ribosyltransferases. The enzymatic function of sirtuins is not yet completely understood but recent reports of histone-activated SIR2-mediated NAD+ metabolism and NAD+-activated SIR2-mediated histone deacetylation suggest a possible coupled reciprocal activation mechanism involving interactions of SIR2 with NAD+ and the N-ε-acetyl-lysine groups of acetylated histone.  

     

    Brand:
    Cayman
    SKU:13477 - 1 ea

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  • Smac is a mitochondrial protein involved in the initiation of apoptosis.{8246} It is a ubiquitously expressed α-helical protein that forms homodimers.{8246,59207} It is localized to the mitochondria but is released following apoptotic stimuli into the cytoplasm, where it binds to inhibitor of apoptosis proteins (IAPs), which are overexpressed in a variety of cancer cells.{8246,59208} Smac binding to IAPs blocks their inhibitory effect on caspase-3, -7, and -9 and leads to the activation of apoptosis.{59209,59208} Knockout of DIABLO, the gene encoding Smac, in colon cancer cells in vitro induces resistance to apoptosis induced by NSAIDs and TRAIL, but not cisplatin (Item No. 13119), mitomycin C (Item No. 11435), or camptothecin (Item No. 11694).{59210} DIABLO knockout in mice does not induce changes in apoptosis or tumorigenesis, indicating a redundancy of its function.{59208} Overexpression of DIABLO in vitro does not affect basal levels of apoptosis but increases the sensitivity of cells to other apoptotic stimuli.{59211} Cayman’s Smac Rabbit Monoclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications.  

     

    Brand:
    Cayman
    SKU:32221 - 100 µl

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  • SMADs are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily.{19268} SMADs can be divided into receptor-regulated SMADs (R-SMADs:SMAD1, SMAD2, SMAD3, SMAD5, SMAD8, and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8, and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature. This antibody is expected to detect SMAD 1, 5, 8, and 9.  

     

    Brand:
    Cayman
    SKU:10822 - 100 µg

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  • SMAD2 is an intracellular protein belonging to the Dwarfin (DWA/B)/SMAD family. SMAD2 associates with SMAD4 for translocation to the nucleus. It acts as an intracellular mediator of the TGFβ family of cytokines and activin type 1 receptor (ACVR1C). Hence it regulates multiple cellular processes like cell growth, proliferation, differentiation, and apoptosis and also cooperates with transcription factors to regulate expression of defined genes. Apart from playing an important role in TGFβ1-induced CTGF expression, SMADs also act as markers of epithelial-mesenchymal transition. SMAD2 also acts as an integrator of multiple signals in the regulation of NOS3 expression. It is ubiquitously expressed in most of tissues and may contribute to colorectal, hepatocellular, lung, and breast carcinomas as well as cervical cancer.  

     

    Brand:
    Cayman
    SKU:10823 - 100 µg

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  • SMAD3, a member of the SMAD family of proteins, is involved in the TGF-β signaling cascade. A human homolog of Drosophila Mad protein, SMAD3 consists of an N-terminal DNA-binding MH1 domain, a linker region and a C-terminal MH2 domain. It is a receptor-regulated SMAD (R-SMAD) that functions downstream of TGF-β and activin receptors and mediates their signaling.{19270,19289} It is recruited by SARA (SMAD anchor for receptor activation) to the receptor kinase for phosphorylation. Upon phosphorylation, SMAD3 dissociates from SARA, forms a complex with SMAD4 and transmigrates into the nucleus where it complexes with other cofactors and acts as a transcription factor. It has an indispensable role in cell proliferation, differentiation, apoptosis and formation of ECM. Loss of SMAD3 results in childhood T cell leukemia.  

     

    Brand:
    Cayman
    SKU:10832 - 100 µg

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