ELISA Kits
Showing 1951–2100 of 3623 results
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Necrosis is accompanied by mitochondrial swelling and increased plasma membrane permeability, while apoptosis involves an articulated breakdown of the cell into membrane-bound apoptotic bodies. Lactate dehydrogenase (LDH) is a soluble cytosolic enzyme that is released into the culture medium following loss of membrane integrity resulting from cell- or compound-induced cytotoxicity. LDH activity, therefore, can be used as an indicator of cell membrane integrity and serve as a general means to assess cell viability by measuring plasma membrane permeability. Cayman’s LDH Cytotoxicity Assay Kit measures cell death in response to chemical compounds or environmental factors using a coupled two-step reaction. In the first step, LDH catalyzes the reduction of NAD+ to NADH and H+ by oxidation of lactate to pyruvate. In the second step of the reaction, diaphorase uses the newly-formed NADH and H+ to catalyze the reduction of a tetrazolium salt (INT) to highly-colored formazan which absorbs strongly at 490-520 nm.
Brand:CaymanSKU:601170 - 96 wellsAvailable on backorder
Cholesterol is an essential element of cell membranes and is carried around the body packaged in lipoproteins, primarily low-density lipoproteins (LDLs). The LDL receptors (LDLRs) are cell surface glycoproteins that scavenge LDL from the blood and regulate plasma LDL cholesterol. LDLRs contain five primary domains: the ligand binding domain, the homology with the EGF precursor domain, the O-linked sugars domain, the membrane-spanning region, and a cytoplasmic tail.{13847} In humans, more than 60% of the LDL R3 are found in the liver.{13846} LDLR expression is under hormonal control both in vivo and in vitro. Mutations in the LDLR gene cause disorders such as familial hypercholesterolemia and atherosclerosis.{13847} Murine LDLR is 864 amino acids in length with an estimated molecular weight of 95 kDa. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.{13847} Cayman’s LDL receptor polyclonal antibody detects both glycosylated and unglycosylated proteins in tissue/cell samples such as liver, HepG2, and RAW 264.7 cells.
Brand:CaymanSKU:10007665 - 1 eaAvailable on backorder
Cholesterol is an essential element of cell membranes and is carried around the body packaged in lipoproteins, primarily low-density lipoproteins (LDLs). The LDL receptors (LDLRs) are cell surface glycoproteins that scavenge LDL from the blood and regulate plasma LDL cholesterol. LDLRs contain five primary domains: the ligand binding domain, the homology with the EGF precursor domain, the O-linked sugars domain, the membrane-spanning region, and a cytoplasmic tail.{13847} In humans, more than 60% of the LDL R3 is found in the liver.{13846} LDLR expression is under hormonal control both in vivo and in vitro. Mutations in the LDLR gene cause disorders such as familial hypercholesterolemia and atherosclerosis.{13847} Murine LDLR is 864 amino acids in length with an estimated molecular weight of 95 kDa. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.{13847} Cayman’s LDL receptor polyclonal antibody detects both glycosylated and unglycosylated proteins in tissue/cell samples such as liver, HepG2, and RAW 264.7 cells.
Brand:CaymanSKU:22727 - 50 µgAvailable on backorder
Low-density lipoproteins (LDL) is the major carrier of cholesterol in the blood, accounting for more than 60% of total plasma cholesterol. LDL is taken up by hepatic and extrahepatic tissues through LDL receptor-mediated endocytosis where it is internalized and degraded to free cholesterol and amino acids. Uptake of native and oxidized LDL by monocyte-derived macrophages in the vessel wall results in accumulation of cholesterol and formation of atherosclerotic plaques. The mechanism underlying the uptake of LDL by both hepatic tissue and cells of the cardiovascular system is not clearly understood. Thus, LDL uptake and its regulation are important therapeutic targets for atherosclerosis and related diseases. Cayman’s LDL Uptake Cell-Based Assay Kit provides a convenient tool for studying LDL uptake and regulation at cellular level. The kit employs human LDL conjugated to DyLight™ 550 as a fluorescent probe for detection of LDL uptake into cultured cells. A LDL receptor-specific polyclonal antibody and a DyLight™ 488-conjugated secondary antibody are included for identifying the distribution of LDL receptors.
Brand:CaymanSKU:10011125 - 1 eaAvailable on backorder
Cayman’s LDL Uptake Flow Cytometry Assay Kit employs human LDL conjugated to DyLight™ 488 as a convenient tool for studying the uptake of LDL in cultured cells. Flow cytometry provides the advantage of assessing the uptake of LDL at the single-cell level. In addition, multiplexing with other markers, such as LDLR expression, is possible to gain more information from a single experiment. Lovastatin is included in this kit as a control modulator of LDL uptake by hepatocytes. The reagents provided in this kit are sufficient to test 48 samples by flow cytometry.
Brand:CaymanSKU:601470 - 48 testsAvailable on backorder
Leptin, the product of the ob (obese) gene, is produced mainly in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism, and body weight regulation. The primary effect of leptin appears to be mediated by leptin receptors expressed mainly in the hypothalamus. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action; it decreases the appetite and increases energy consumption. Leptin is secreted in circadian fashion with nocturnal rise in both lean and obese patients. Mutations of the ob gene resulting in leptin deficiency are the cause of obesity in the ob/ob mice suggesting that endogeneous leptin can normalize their body weight. In contrast, human obese subjects may have high level of leptin, indicating a mechanism of leptin resistance. This Enzyme Immunometric Assay (EIA) is based on a double-antibody sandwich technique. The wells of the plate supplied with the kit are coated with a polyclonal antibody specific of human leptin. This antibody will bind any Leptin introduced in the wells (sample or standard). [Bertin Catalog No. A05174]
Brand:CaymanSKU:500010 - 96 wellsAvailable on backorder
Leptin is a 16 kDa protein hormone encoded by the obese (ob) gene with important effects in metabolism and regulating body weight. Leptin has dual actions, decreasing appetite and increasing energy consumption. Mutations in the ob gene or leptin receptor gene causes hyperphagia, reduced energy expenditure, and severe obesity. Studies have also shown that leptin is a regulator of reproductive function, bone metabolism and plays a significant role as an immunomodulator. This Enzyme Immunometric Assay (EIA) is based on a double-antibody sandwich technique specific for mouse and rat leptin. [Bertin Catalog No. A05176]
Brand:CaymanSKU:10007609 - 96 wellsAvailable on backorder
Leptin, the product of the ob (obese) gene, is produced mainly in adipose tissue and is considered to play an important role in appetite control, fat metabolism and body weight regulation. The primary effect of leptin appears to be mediated by leptin receptors expressed mainly in the hypothalamus. This Enzyme Immunometric Assay (EIA) is based on a double-antibody sandwich technique specific for the human leptin receptor. The assay utilizes plates coated with a polyclonal antibody specific for the human leptin receptor and a HRP-conjugated polyclonal antibody for detection. [Bertin Catalog No. A05175]
Brand:CaymanSKU:10007608 - 96 wellsAvailable on backorder
Leukotriene A4 hydrolase (LTA4 hydrolase) has been cloned from a variety of species including human, rat, and guinea pig.{969,4144,4143,4142} These species exhibit ~90% homology at the amino acid level. Human LTA4 hydrolase has a calculated molecular mass of 69 kDa based on the deduced amino acid sequence.{969,4144}
Brand:CaymanSKU:160250 - 500 µlAvailable on backorder
Brand:CaymanSKU:420112 - 100 dtnAvailable on backorder
Brand:CaymanSKU:420112 - 500 dtnAvailable on backorder
Leukotriene B4 (LTB4) is synthesized from arachidonic acid by the combined action of 5-lipoxygenase and LTA4 hydrolase.{1719} LTB4 has long been recognized as a potent mediator of inflammation. It stimulates a number of leukocyte functions, including aggregation, stimulation of ion fluxes, enhancement of lysosomal enzyme release, superoxide anion production, chemotaxis, and chemokinesis.{329,1803} In subnanomolar ranges (3.9 x 10−10 M), LTB4 causes chemotaxis and chemokinesis in human PMNLs.{329} At higher concentrations, (1.0 x 10−7 M), LTB4 leads to neutrophil aggregation and degranulation as well as superoxide anion production.{329,83} Plasma levels of LTB4 increase from 100 ng/ml following leukocyte stimulation.{1534,1454,1346} LTB4 is metabolized in leukocytes and hepatocytes to less active 20-hydroxy and 20-carboxy LTB4 by NADPH-dependent cytochrome P450 enzymes followed by β-oxidation at the ω-end to ω-carboxy dinor LTB4 and ω-carboxy tetranor LTB3.{1803,589,1032,377} LTB4 is not excreted in the urine.{1465}
Brand:CaymanSKU:520111 - 480 solid wellsAvailable on backorder
Leukotriene B4 (LTB4) is synthesized from arachidonic acid by the combined action of 5-lipoxygenase and LTA4 hydrolase.{1719} LTB4 has long been recognized as a potent mediator of inflammation. It stimulates a number of leukocyte functions, including aggregation, stimulation of ion fluxes, enhancement of lysosomal enzyme release, superoxide anion production, chemotaxis, and chemokinesis.{329,1803} In subnanomolar ranges (3.9 x 10−10 M), LTB4 causes chemotaxis and chemokinesis in human PMNLs.{329} At higher concentrations, (1.0 x 10−7 M), LTB4 leads to neutrophil aggregation and degranulation as well as superoxide anion production.{329,83} Plasma levels of LTB4 increase from 100 ng/ml following leukocyte stimulation.{1534,1454,1346} LTB4 is metabolized in leukocytes and hepatocytes to less active 20-hydroxy and 20-carboxy LTB4 by NADPH-dependent cytochrome P450 enzymes followed by β-oxidation at the ω-end to ω-carboxy dinor LTB4 and ω-carboxy tetranor LTB3.{1803,589,1032,377} LTB4 is not excreted in the urine.{1465}
Brand:CaymanSKU:520111 - 480 strip wellsAvailable on backorder
Leukotriene B4 (LTB4) is synthesized from arachidonic acid by the combined action of 5-lipoxygenase and LTA4 hydrolase.{1719} LTB4 has long been recognized as a potent mediator of inflammation. It stimulates a number of leukocyte functions, including aggregation, stimulation of ion fluxes, enhancement of lysosomal enzyme release, superoxide anion production, chemotaxis, and chemokinesis.{329,1803} In subnanomolar ranges (3.9 x 10−10 M), LTB4 causes chemotaxis and chemokinesis in human PMNLs.{329} At higher concentrations, (1.0 x 10−7 M), LTB4 leads to neutrophil aggregation and degranulation as well as superoxide anion production.{329,83} Plasma levels of LTB4 increase from 100 ng/ml following leukocyte stimulation.{1534,1454,1346} LTB4 is metabolized in leukocytes and hepatocytes to less active 20-hydroxy and 20-carboxy LTB4 by NADPH-dependent cytochrome P450 enzymes followed by β-oxidation at the ω-end to ω-carboxy dinor LTB4 and ω-carboxy tetranor LTB3.{1803,589,1032,377} LTB4 is not excreted in the urine.{1465}
Brand:CaymanSKU:520111 - 96 solid wellsAvailable on backorder
Leukotriene B4 (LTB4) is synthesized from arachidonic acid by the combined action of 5-lipoxygenase and LTA4 hydrolase.{1719} LTB4 has long been recognized as a potent mediator of inflammation. It stimulates a number of leukocyte functions, including aggregation, stimulation of ion fluxes, enhancement of lysosomal enzyme release, superoxide anion production, chemotaxis, and chemokinesis.{329,1803} In subnanomolar ranges (3.9 x 10−10 M), LTB4 causes chemotaxis and chemokinesis in human PMNLs.{329} At higher concentrations, (1.0 x 10−7 M), LTB4 leads to neutrophil aggregation and degranulation as well as superoxide anion production.{329,83} Plasma levels of LTB4 increase from 100 ng/ml following leukocyte stimulation.{1534,1454,1346} LTB4 is metabolized in leukocytes and hepatocytes to less active 20-hydroxy and 20-carboxy LTB4 by NADPH-dependent cytochrome P450 enzymes followed by β-oxidation at the ω-end to ω-carboxy dinor LTB4 and ω-carboxy tetranor LTB3.{1803,589,1032,377} LTB4 is not excreted in the urine.{1465}
Brand:CaymanSKU:520111 - 96 strip wellsAvailable on backorder
Brand:CaymanSKU:420114 - 1 eaAvailable on backorder
LTB4 stimulates a number of leukocyte functions, including aggregation, stimulation of ion fluxes, enhancement of lysosomal enzyme release, superoxide anion production, chemotaxis, and chemokinesis. Plasma levels of LTB4 increase from 100 ng/ml following leukocyte stimulation. LTB4 is metabolized in leukocytes and hepatocytes to less active 20-hydroxy and 20-carboxy LTB4 and then further oxidized to ω-carboxy dinor LTB4 and ω-carboxy tetranor-LTB3. LTB4 is not excreted in the urine. Cayman’s LTB4 Express ELISA Kit is a competitive assay that can be used for quantification of LTB4 in plasma and other sample matrices. The assay has a range from 15.6-2,000 pg/ml and a sensitivity (80% B/B0) of approximately 45 pg/ml.
Brand:CaymanSKU:10009292 - 480 solid wellsAvailable on backorder
LTB4 stimulates a number of leukocyte functions, including aggregation, stimulation of ion fluxes, enhancement of lysosomal enzyme release, superoxide anion production, chemotaxis, and chemokinesis. Plasma levels of LTB4 increase from 100 ng/ml following leukocyte stimulation. LTB4 is metabolized in leukocytes and hepatocytes to less active 20-hydroxy and 20-carboxy LTB4 and then further oxidized to ω-carboxy dinor LTB4 and ω-carboxy tetranor-LTB3. LTB4 is not excreted in the urine. Cayman’s LTB4 Express ELISA Kit is a competitive assay that can be used for quantification of LTB4 in plasma and other sample matrices. The assay has a range from 15.6-2,000 pg/ml and a sensitivity (80% B/B0) of approximately 45 pg/ml.
Brand:CaymanSKU:10009292 - 480 strip wellsAvailable on backorder
LTB4 stimulates a number of leukocyte functions, including aggregation, stimulation of ion fluxes, enhancement of lysosomal enzyme release, superoxide anion production, chemotaxis, and chemokinesis. Plasma levels of LTB4 increase from 100 ng/ml following leukocyte stimulation. LTB4 is metabolized in leukocytes and hepatocytes to less active 20-hydroxy and 20-carboxy LTB4 and then further oxidized to ω-carboxy dinor LTB4 and ω-carboxy tetranor-LTB3. LTB4 is not excreted in the urine. Cayman’s LTB4 Express ELISA Kit is a competitive assay that can be used for quantification of LTB4 in plasma and other sample matrices. The assay has a range from 15.6-2,000 pg/ml and a sensitivity (80% B/B0) of approximately 45 pg/ml.
Brand:CaymanSKU:10009292 - 96 solid wellsAvailable on backorder
LTB4 stimulates a number of leukocyte functions, including aggregation, stimulation of ion fluxes, enhancement of lysosomal enzyme release, superoxide anion production, chemotaxis, and chemokinesis. Plasma levels of LTB4 increase from 100 ng/ml following leukocyte stimulation. LTB4 is metabolized in leukocytes and hepatocytes to less active 20-hydroxy and 20-carboxy LTB4 and then further oxidized to ω-carboxy dinor LTB4 and ω-carboxy tetranor-LTB3. LTB4 is not excreted in the urine. Cayman’s LTB4 Express ELISA Kit is a competitive assay that can be used for quantification of LTB4 in plasma and other sample matrices. The assay has a range from 15.6-2,000 pg/ml and a sensitivity (80% B/B0) of approximately 45 pg/ml.
Brand:CaymanSKU:10009292 - 96 strip wellsAvailable on backorder
Brand:CaymanSKU:401072 - 100 dtnAvailable on backorder
Brand:CaymanSKU:401072 - 500 dtnAvailable on backorder
Leukotrienes (LTs) are a group of acute inflammatory mediators derived from arachidonic acid in eosinophils, mast cells, and macrophages. Their biosynthesis proceeds via the 5-lipoxygenase (5-LO) pathway in which 5-LO converts arachidonic acid into LTA4 with 5(S)-HpETE as an intermediate. The conjugation of glutathione to LTA4 results in the formation of LTC4, which is then rapidly metabolized to LTD4 and LTE4. This metabolism is essentially complete within 10 minutes in the human lung. Cayman’s LTC4 ELISA Kit is a competitive assay that can be used for quantification of LTC4 in culture media and other sample matrices. The assay has a range from 17.1-5,000 pg/ml and a sensitivity (80% B/B0) value of approximately 40 pg/ml.
Brand:CaymanSKU:501070 - 480 solid wellsAvailable on backorder
Leukotrienes (LTs) are a group of acute inflammatory mediators derived from arachidonic acid in eosinophils, mast cells, and macrophages. Their biosynthesis proceeds via the 5-lipoxygenase (5-LO) pathway in which 5-LO converts arachidonic acid into LTA4 with 5(S)-HpETE as an intermediate. The conjugation of glutathione to LTA4 results in the formation of LTC4, which is then rapidly metabolized to LTD4 and LTE4. This metabolism is essentially complete within 10 minutes in the human lung. Cayman’s LTC4 ELISA Kit is a competitive assay that can be used for quantification of LTC4 in culture media and other sample matrices. The assay has a range from 17.1-5,000 pg/ml and a sensitivity (80% B/B0) value of approximately 40 pg/ml.
Brand:CaymanSKU:501070 - 480 strip wellsAvailable on backorder
Leukotrienes (LTs) are a group of acute inflammatory mediators derived from arachidonic acid in eosinophils, mast cells, and macrophages. Their biosynthesis proceeds via the 5-lipoxygenase (5-LO) pathway in which 5-LO converts arachidonic acid into LTA4 with 5(S)-HpETE as an intermediate. The conjugation of glutathione to LTA4 results in the formation of LTC4, which is then rapidly metabolized to LTD4 and LTE4. This metabolism is essentially complete within 10 minutes in the human lung. Cayman’s LTC4 ELISA Kit is a competitive assay that can be used for quantification of LTC4 in culture media and other sample matrices. The assay has a range from 17.1-5,000 pg/ml and a sensitivity (80% B/B0) value of approximately 40 pg/ml.
Brand:CaymanSKU:501070 - 96 solid wellsAvailable on backorder
Leukotrienes (LTs) are a group of acute inflammatory mediators derived from arachidonic acid in eosinophils, mast cells, and macrophages. Their biosynthesis proceeds via the 5-lipoxygenase (5-LO) pathway in which 5-LO converts arachidonic acid into LTA4 with 5(S)-HpETE as an intermediate. The conjugation of glutathione to LTA4 results in the formation of LTC4, which is then rapidly metabolized to LTD4 and LTE4. This metabolism is essentially complete within 10 minutes in the human lung. Cayman’s LTC4 ELISA Kit is a competitive assay that can be used for quantification of LTC4 in culture media and other sample matrices. The assay has a range from 17.1-5,000 pg/ml and a sensitivity (80% B/B0) value of approximately 40 pg/ml.
Brand:CaymanSKU:501070 - 96 strip wellsAvailable on backorder
Brand:CaymanSKU:401062 - 100 dtnAvailable on backorder
Brand:CaymanSKU:401062 - 500 dtnAvailable on backorder
Leukotriene E4 (LTE4) is a product of the 5-lipoxygenase (5-LO) pathway in activated mast cells, eosinophils, and monocytes. LTA4, the primary 5-LO metabolite, is rapidly converted to LTC4 and sequentially to LTD4 and LTE4. While plasma levels of LTC4 are virtually undetectable, exogenously administered LTC4 is recovered in the urine as LTE4 (5-13%) and two prominent oxidized metabolites resulting from several cycles of β-oxidation. Cayman’s LTE4 ELISA Kit is a competitive assay developed for the measurement of LTE4 from urine without a purification step. The assay has a range of 7.8-1,000 pg/ml and a sensitivity (80% B/B0) of approximately 25 pg/ml. This “dilute and go” format has been used successfully to demonstrate significant and reproducible changes in LTE4 levels in the urine of asthmatic patients after antigen challenge. If your samples require additional purification prior to analysis, Cayman’s Cysteinyl Leukotriene Affinity Sorbent (Item No. 400396) can be used for this purpose.
Brand:CaymanSKU:501060 - 480 solid wellsAvailable on backorder
Leukotriene E4 (LTE4) is a product of the 5-lipoxygenase (5-LO) pathway in activated mast cells, eosinophils, and monocytes. LTA4, the primary 5-LO metabolite, is rapidly converted to LTC4 and sequentially to LTD4 and LTE4. While plasma levels of LTC4 are virtually undetectable, exogenously administered LTC4 is recovered in the urine as LTE4 (5-13%) and two prominent oxidized metabolites resulting from several cycles of β-oxidation. Cayman’s LTE4 ELISA Kit is a competitive assay developed for the measurement of LTE4 from urine without a purification step. The assay has a range of 7.8-1,000 pg/ml and a sensitivity (80% B/B0) of approximately 25 pg/ml. This “dilute and go” format has been used successfully to demonstrate significant and reproducible changes in LTE4 levels in the urine of asthmatic patients after antigen challenge. If your samples require additional purification prior to analysis, Cayman’s Cysteinyl Leukotriene Affinity Sorbent (Item No. 400396) can be used for this purpose.
Brand:CaymanSKU:501060 - 480 strip wellsAvailable on backorder
Leukotriene E4 (LTE4) is a product of the 5-lipoxygenase (5-LO) pathway in activated mast cells, eosinophils, and monocytes. LTA4, the primary 5-LO metabolite, is rapidly converted to LTC4 and sequentially to LTD4 and LTE4. While plasma levels of LTC4 are virtually undetectable, exogenously administered LTC4 is recovered in the urine as LTE4 (5-13%) and two prominent oxidized metabolites resulting from several cycles of β-oxidation. Cayman’s LTE4 ELISA Kit is a competitive assay developed for the measurement of LTE4 from urine without a purification step. The assay has a range of 7.8-1,000 pg/ml and a sensitivity (80% B/B0) of approximately 25 pg/ml. This “dilute and go” format has been used successfully to demonstrate significant and reproducible changes in LTE4 levels in the urine of asthmatic patients after antigen challenge. If your samples require additional purification prior to analysis, Cayman’s Cysteinyl Leukotriene Affinity Sorbent (Item No. 400396) can be used for this purpose.
Brand:CaymanSKU:501060 - 96 solid wellsAvailable on backorder
Leukotriene E4 (LTE4) is a product of the 5-lipoxygenase (5-LO) pathway in activated mast cells, eosinophils, and monocytes. LTA4, the primary 5-LO metabolite, is rapidly converted to LTC4 and sequentially to LTD4 and LTE4. While plasma levels of LTC4 are virtually undetectable, exogenously administered LTC4 is recovered in the urine as LTE4 (5-13%) and two prominent oxidized metabolites resulting from several cycles of β-oxidation. Cayman’s LTE4 ELISA Kit is a competitive assay developed for the measurement of LTE4 from urine without a purification step. The assay has a range of 7.8-1,000 pg/ml and a sensitivity (80% B/B0) of approximately 25 pg/ml. This “dilute and go” format has been used successfully to demonstrate significant and reproducible changes in LTE4 levels in the urine of asthmatic patients after antigen challenge. If your samples require additional purification prior to analysis, Cayman’s Cysteinyl Leukotriene Affinity Sorbent (Item No. 400396) can be used for this purpose.
Brand:CaymanSKU:501060 - 96 strip wellsAvailable on backorder
Brand:CaymanSKU:420414 - 1 eaAvailable on backorder
Lipases perform essential roles in the digestion, transportation, and processing of dietary lipids by controlling the clearance of triglyceride-rich lipoproteins from the circulation. Manipulating lipolysis has therapeutic potential in the metabolic disorders frequently associated with obesity. Cayman’s Lipase Activity Assay provides a fluorescence-based method for detecting lipase activity in plasma, serum, tissue homogenates, and cell culture samples. In the assay, lipase hydrolyzes arachidonoyl-1-thioglycerol to arachidonic acid and thioglycerol. Thioglycerol reacts with the thiol fluorometric detector to yield a highly fluorescent product which can be analyzed with an excitation wavelength of 380-390 nm and an emission wavelength of 510-520 nm.
Brand:CaymanSKU:700640 - 96 wellsAvailable on backorder
Brand:CaymanSKU:600046 - 30 mlAvailable on backorder
Brand:CaymanSKU:10008981 - 1 eaAvailable on backorder
Brand:CaymanSKU:600045 - 25 mlAvailable on backorder
Brand:CaymanSKU:600056 - 5 eaAvailable on backorder
Brand:CaymanSKU:600044 - 30 mlAvailable on backorder
Cayman’s Lipid Droplets Fluorescence Assay Kit can be used to study regulators of lipid droplet biogenesis. The main advantage of this assay is that the green fluorescence of Nile Red is both very sensitive and specific for lipid droplets.{17124} Furthermore, changes in lipid droplet biogenesis in various cell types in response to different manipulations can be both qualified and quantified by fluorescence microscopy, flow cytometry, or fluorescent plate readers. Simultaneous visualization of lipid droplets and associated proteins is also possible when used with antibodies conjugated to compatible fluorophores. Oleic acid, which is commonly used to induce lipid droplet formation in cultured cells, is included as a positive control. This kit provides sufficient reagents to effectively treat/stain 480 individual wells of cells when utilized in a 96-well plate format.
Brand:CaymanSKU:500001 - 480 testsAvailable on backorder
Quantification of lipid peroxidation is essential to assess the role of oxidative injury in pathophysiological disorders.{1240,2997,4988} Lipid peroxidation results in the formation of highly reactive and unstable hydroperoxides of both saturated and unsaturated lipids. Our Lipid Hydroperoxide Assay Kit measures the hydroperoxides directly utilizing the redox reactions with ferrous ions.{3237} An easy to use quantitative extraction method was developed to extract lipid hydroperoxides into chloroform, and the extract is directly used in the assay. This procedure eliminates any interference caused by hydrogen peroxide or endogenous ferric ions in the sample and provides a sensitive and reliable assay for lipid peroxidation. This kit is designed for use with either a single-tube spectrophotometer to read the results or with a 96 well microplate reader. The plate used with the microplate reader is a reusable glass plate which is available with the purchase of Item No. 705003. The range of the assay is 0.25-5 nmol hydroperoxide per tube.
Brand:CaymanSKU:705002 - 100 dtnAvailable on backorder
Quantification of lipid peroxidation is essential to assess the role of oxidative injury in pathophysiological disorders.{1240,2997,4988} Lipid peroxidation results in the formation of highly reactive and unstable hydroperoxides of both saturated and unsaturated lipids. Our Lipid Hydroperoxide Assay Kit measures the hydroperoxides directly utilizing the redox reactions with ferrous ions.{3237} An easy to use quantitative extraction method was developed to extract lipid hydroperoxides into chloroform, and the extract is directly used in the assay. This procedure eliminates any interference caused by hydrogen peroxide or endogenous ferric ions in the sample and provides a sensitive and reliable assay for lipid peroxidation. This kit is available in two formats: 100 dtn, which is designed to be read using a single-tube spectrophotometer, and 96 wells, which is designed to be read using a 96-well microplate reader. The 96 well kit contains a special glass plate which is resistant to organic solvents used in the assay; this is the only difference between the two kit types.
Brand:CaymanSKU:705003 - 96 wellsAvailable on backorder
Lipoxin A4 (LXA4; Item No. 90410) is a part of the specialized pro-resolving mediator (SPM) family of polyunsaturated fatty acid (PUFA) metabolites.{35079} It is formed from arachidonic acid through double lipoxygenase-catalyzed reactions initiated by either 5-lipoxygenase (5-LO) followed by 12-LO/15-LO, with a leukotriene A4 (LTA4) intermediate, or by 15-LO followed by 5-LO, with 15(S)-HETE and 5(S)-Hp-15(S)-HETE intermediates. The generation of LXA4 typically requires transcellular metabolism of arachidonic acid.{35079,35339} For example, LTA4 that is synthesized in neutrophils by 5-LO is metabolized to LXA4 in platelets by 12-LO.{35339} LXA4 is released during the resolution phase of inflammation and binds to the G protein-coupled LXA4 receptor (ALX)/formyl peptide receptor (FPR2), which is located on leukocytes, and induces cell type-specific signaling.1 In macrophages, it activates phosphatidylinositol 3-kinase (PI3K) and AKT, stimulates non-phlogistic phagocytosis of apoptotic leukocytes, and inhibits apoptosis to prolong the phagocytotic phase.{11078,35388} In neutrophils, it inhibits chemotaxis and transmigration and inhibits production of LTB4 (Item No. 20110).{35389} Cayman’s LXA4 ELISA Kit is a competitive assay which can be used for quantification of LXA4. This assay has been tested in urine, serum, and plasma. The ELISA typically displays an IC50 (50% B/B0) of approximately 200 pg/ml and a detection limit (80% B/B0) of approximately 50 pg/ml.
Brand:CaymanSKU:590410 - 96 solid wellAvailable on backorder
Lipoxin A4 (LXA4; Item No. 90410) is a part of the specialized pro-resolving mediator (SPM) family of polyunsaturated fatty acid (PUFA) metabolites.{35079} It is formed from arachidonic acid through double lipoxygenase-catalyzed reactions initiated by either 5-lipoxygenase (5-LO) followed by 12-LO/15-LO, with a leukotriene A4 (LTA4) intermediate, or by 15-LO followed by 5-LO, with 15(S)-HETE and 5(S)-Hp-15(S)-HETE intermediates. The generation of LXA4 typically requires transcellular metabolism of arachidonic acid.{35079,35339} For example, LTA4 that is synthesized in neutrophils by 5-LO is metabolized to LXA4 in platelets by 12-LO.{35339} LXA4 is released during the resolution phase of inflammation and binds to the G protein-coupled LXA4 receptor (ALX)/formyl peptide receptor (FPR2), which is located on leukocytes, and induces cell type-specific signaling.1 In macrophages, it activates phosphatidylinositol 3-kinase (PI3K) and AKT, stimulates non-phlogistic phagocytosis of apoptotic leukocytes, and inhibits apoptosis to prolong the phagocytotic phase.{11078,35388} In neutrophils, it inhibits chemotaxis and transmigration and inhibits production of LTB4 (Item No. 20110).{35389} Cayman’s LXA4 ELISA Kit is a competitive assay which can be used for quantification of LXA4. This assay has been tested in urine, serum, and plasma. The ELISA typically displays an IC50 (50% B/B0) of approximately 200 pg/ml and a detection limit (80% B/B0) of approximately 50 pg/ml.
Brand:CaymanSKU:590410 - 96 strip wellAvailable on backorder
Cayman’s Lipoxin B4 (LXB4) ELISA Kit is a competitive assay that can be used for quantification of LXB4 in plasma, serum, and urine. The assay has a range of 1.6-1,000 pg/ml with a midpoint (50% B/B0) of approximately 41 pg/ml and a sensitivity (80% B/B0) of approximately 9.5 pg/ml.
Brand:CaymanSKU:501920 - 96 solid wellAvailable on backorder
Cayman’s Lipoxin B4 (LXB4) ELISA Kit is a competitive assay that can be used for quantification of LXB4 in plasma, serum, and urine. The assay has a range of 1.6-1,000 pg/ml with a midpoint (50% B/B0) of approximately 41 pg/ml and a sensitivity (80% B/B0) of approximately 9.5 pg/ml.
Brand:CaymanSKU:501920 - 96 strip wellAvailable on backorder
Lipoxin B4 ELISA Polyclonal Antiserum has been tested and formulated to work exclusively with Cayman’s Lipoxin B4 ELISA Kit (Item No. 501920). Please visit Lipoxin B4 ELISA Kit (Item No. 501920) for the kit protocol, procedures, and product handling.
Brand:CaymanSKU:401922 - 100 dtnAvailable on backorder
Lipoxin B4 ELISA Standard has been tested and formulated to work exclusively with Cayman’s Lipoxin B4 ELISA Kit (Item No. 501920). Please visit Lipoxin B4 ELISA Kit (Item No. 501920) for the kit protocol, procedures, and product handling.
Brand:CaymanSKU:401924 - 25 ngAvailable on backorder
Brand:CaymanSKU:760710 - 1 eaAvailable on backorder
Lipoxygenases are members of a class of non-heme iron-containing dioxygenases that catalyze the addition of molecular oxygen to fatty acids containing a cis,cis-1,4-pentadiene system to give an unsaturated fatty acid hydroperoxide. In mammals, lipoxygenases carry out the first step in the arachidonic acid cascade.{3140,2972} 5- and 15-LOs lead to the biologically active lipoxins, whereas 5-LO leads to 5,6-epoxy-leukotrienes which are involved in a variety of inflammatory responses, including neutrophil chemotaxis, vascular permeability, and smooth muscle contraction.{482} In contrast, Nassar, et al., suggested that animal 15-LO products act as anti-inflammatory agents.{1390} This implies that the 5- and 15-LO pathways may play a role in regulating inflammation. To elucidate the role of each lipoxygenase, it is particularly important to develop inhibitors that are enzyme specific. This assay kit provides an accurate and convenient method for screening lipoxygenase inhibitors. This assay measures the hydroperoxides generated from the incubation of a lipoxygenase (5-, 12-, or 15-LO) with either arachidonic or linoleic acid.
Brand:CaymanSKU:760700 - 96 wellsAvailable on backorder
LL-37 is an antimicrobial peptide cleaved from the only described human cathelicidin, hCAP18. Production of LL-37 is activated by M. tuberculosis infection in neutrophils and epithelial cells of the lung. {17198} LL-37 is a component of neutrophil extracellular traps (NETs), and its presence on NETs has been associated with the activation of inflammation and NLRP3 inflammasomes. {26640} Autoimmune disorders such as systemic lupus erythematosus have been associated with the generation of autoantibodies against NET proteins. {26640} LL-37 has a predicted molecular weight of 5 kDa; Cayman’s LL-37 Polyclonal Antibody detects a 5 kDa band, as well as an 18 kDa band corresponding to the hCAP18 precursor to LL-37.
Brand:CaymanSKU:15637 - 1 eaAvailable on backorder
LPA1 (also known as EDG-2) is one of four known LPA receptors (LPA1-4).{13001} LPA1-3 are part a family of G protein-coupled receptors that share identity to the sphingosine-1-phosphate receptors (S1P1-5).{11049} LPA1 couples with three types of G proteins, Gi/o, Gq, and G12/13 to induce a range of cellular responses including activation of phospholipase C, multiple kinases, the serum response element and cell proliferation.{11808,12477} LPA1 mRNA is detected a wide range of tissues and cells including brain, heart, small intestine, spleen, testis, kidney, HeLa cells, and A549 cells.{11810,12912} Mouse and human LPA1 have 364 amino acids with an estimated molecular weight of 41 kDa.{12477} Cayman’s LPA1 Polyclonal Antibody detects the protein from several species from 43-50 kDa, suggesting heterogeneous LPA1 post-translational modifications in distinct cells.
Brand:CaymanSKU:10005280 - 1 eaAvailable on backorder
Lysophosphatidic acid receptor 3 (LPA3, also known as EDG-7) is one of three LPA receptors (LPA1, LPA2, and LPA3) that are members of large family of G protein-coupled receptors that also include those for sphingosine-1-phosphate (S1P1-5).{11049} The LPA receptors mediate many cellular responses including cytoskeletal rearrangements, cell proliferation, and inhibition of gap junction communication.{11808,11809} Mouse and human LPA3 have 353 amino acids with an estimated molecular weight of 40 kDa.{11811,11810} The mRNA level of LPA3 is high in testes, kidney, and lung but low in intestine, heart, thymus, and stomach.{11810} Cayman Chemical’s LPA3 polyclonal antibody can be used for western blot, immunofluorescence, and immunocytochemical analysis for LPA3 on samples of human, mouse, and rat origin. Cayman’s LPA3 Polyclonal Antibody detects a protein at around 40 kDa in human HepG2 cells, mouse macrophages, and in mouse and rat liver.
Brand:CaymanSKU:10004840 - 1 eaAvailable on backorder
Brand:CaymanSKU:705016 - 1 eaAvailable on backorder
Brand:CaymanSKU:705010 - 1 eaAvailable on backorder
Brand:CaymanSKU:705012 - 1 eaAvailable on backorder
Brand:CaymanSKU:700122 - 1 eaAvailable on backorder
Brand:CaymanSKU:700121 - 1 eaAvailable on backorder
Brand:CaymanSKU:700123 - 1 eaAvailable on backorder
Brand:CaymanSKU:700124 - 1 eaAvailable on backorder
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Lysine-specific demethylase 1 (LSD1) is a histone demethylase whose actions on specific lysine residues repress transcription of chromosomal DNA. LSD1 also inhibits the tumor suppressor activity of p53 by demethylating a specific lysine residue. Inhibitors of LSD1 are important tools used to elucidate mechanisms of transcription and cell cycle progression and have therapeutic potential for treating cancer. Cayman’s LSD1 Inhibitor Screening Assay Kit provides a convenient fluorescence-based method for screening LSD1-specific inhibitors. The assay is based on the multistep enzymatic reaction in which LSD1 first produces H2O2 during the demethylation of lysine 4 on a peptide corresponding to the first 21 amino acids of the N-terminal tail of histone H3. In the presence of horseradish peroxidase, H2O2 reacts with ADHP to produce the highly fluorescent compound resorufin that can be analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.
Brand:CaymanSKU:700120 - 96 wellsAvailable on backorder
LSD1 is a flavin-containing nuclear protein having a N-terminal SWIRM domain, a C-terminal FAD-binding motif, and an amine oxidase domain. It functions as a transcriptional corepressor and catalyzes the flavin-dependent demethylation of Lys4 of histone 3 resulting in the formation of methyl-free lysine and release of formaldehyde. LSD1 has been found to be typically associated with CoREST and histone deacetylases 1 and 4 and participates in the silencing of endogenous neuron-specific genes.
Brand:CaymanSKU:13554 - 1 eaAvailable on backorder
LSD1, the first known lysine-specific histone demethylase, is an 886 amino acid nuclear protein belonging to flavin monoamine oxidase family. It contains a SWIRM domain, a FAD-binding motif and an amine oxidase domaine. This protein is ubiquitously expressed and is a component of several histone deacetylase complexes. LSD1 acts as a component of the CoREST and other transcriptional co-repressor complexes and also plays an important role in silencing neuronal-specific genes in non-neuronal cells. It is also known to demethylate Lys4 of histone H3, a specific tag for epigenetic transcriptional activation. Reports suggest that it plays an important role in stimulating androgen-receptor-dependent transcription converting oxygen to hydrogen peroxide (might use alternative electron acceptors). Along with nuclear FHL2 it serves as a novel biomarker predictive for prostate cancer.
Brand:CaymanSKU:13486 - 1 eaAvailable on backorder
Luteinizing hormone (LH) is a glycoprotein produced by the pituitary gland and consists of two subunits with a total molecular mass of approximately 30 kDa. The α-subunit is identical to the α-subunit of other pituitary hormones, such follicle stimulating hormone (FSH), thyroid stimulating hormone (TSH), and chorionic gonadotropin (hCG). The β-subunit is unique to LH and confers the specific biological activity to the molecule. Luteinizing hormone stimulates ovulation and the development of the corpus luteum, and maintains the function of the corpus luteum during the first two weeks of pregnancy. In males, LH stimulates the production of testosterone by the testis. Cayman’s Luteinizing Hormone ELISA (human) Kit is an immunometric (i.e., sandwich) ELISA which can be used to measure luteinizing hormone within the range of 0.5-200 mIU/ml. This assay offers specific and sensitive analysis of LH in human serum and has not been validated for other types of samples.
Brand:CaymanSKU:500720 - 96 wellsAvailable on backorder
Lysophosphatidic acid (LPA) is an extracellular signaling lipid that evokes multiple biological functions including induction of platelet aggregation, smooth muscle contraction, and stimulation of cell proliferation and chemotaxis.{12477} Lysophospholipase D (lysoPLD) was first discovered in 1999 as the enzyme responsible for generating LPA from lysophosphatidylcholine (LPC).{13068} It was later revealed to be identical to an autocrine motility factor, autotaxin (ATX), which plays a role in tumor progression and metastasis.{13069,13071} LysoPLD/ATX mRNA is widely expressed with highest levels found in brain, ovary, lung, intestine, and testis.{13067,13070} Rat lysoPLD is composed of 885 amino acids with an estimated molecular weight of 101 kDa. The protein is reported to be heavily glycosylated and thus its apparent size on SDS-PAGE may be run as high as 125 kDa. Useful positive controls include cerebrospinal fluid, mouse ascites or seminal plasma.
Brand:CaymanSKU:10005375 - 1 eaAvailable on backorder
Lysosomal acid lipase (LAL) is a lysosomal enzyme that hydrolyzes cholesteryl esters and triglycerides to produce cholesterol, glycerol, and free fatty acids.{42857} LAL deficiency is due to mutations in the LAL gene, LIPA, that lead to decreases in LAL activity. Wolman’s disease is a severe form of LAL deficiency that begins in infancy and is characterized by a nearly complete or complete lack of LAL activity resulting in gastrointestinal disorders, hepatomegaly, and failure to thrive, leading to hypercholesterolemia and fatality within months without treatment. Cholesterol ester storage disorder is a less severe form of LAL deficiency in which LAL activity is reduced but not abolished. It presents later in life and is characterized by gastrointestinal disturbances, dyslipidemia, hepatomegaly, and impaired liver function. Research towards development of methods to detect deficiency in this enzyme has become an important goal in diagnosing and treating individuals with this disorder.
Brand:CaymanSKU:24854 - 1 eaAvailable on backorder
Lysosomes are intracellular organelles that contain enzymes used for the hydrolysis of waste materials and other cellular debris. The pH of the lysosome is ~4.5-4.8 and is optimal for these hydrolytic enzymes. The lysosomal pH gradient is maintained through vacuolar ATPases which pump protons into the lysosomes. Cayman’s Lysosomal Staining Kit (Red Fluorescence) utilizes a red fluorescent dye that permeates the lysosomes based on pH gradient. Once protonated, the dye is unable to leave the lysosome resulting in enhanced fluorescence. Included in this kit is bafilomycin A1, which is an inhibitor of the vacuolar ATPase. Treatment with bafilomycin A1 results in decreased lysosomal fluorescence upon staining with the lysosomal staining reagent.
Brand:CaymanSKU:601810 - 96 wellsAvailable on backorder
Lysozymes are a critical component of the innate immune system, facilitating the breakdown of bacterial cell walls by catalyzing the hydrolysis of peptidoglycan components. Lysozyme C is a component of many animal secretions such as saliva, tears, and mucus.{25779} Elevated serum levels of lysozymes have been associated with rheumatoid arthritis and systemic lupus.{25778} Lysozyme C is a component of neutrophil extracellular traps (NETs), which have the ability to contact and kill pathogens.{24590} Cayman’s Monoclonal Antibody detects lysozyme C in neutrophil-derived NETs.
Brand:CaymanSKU:15641 - 1 eaAvailable on backorder
Brand:CaymanSKU:705194 - 1 eaAvailable on backorder
Brand:CaymanSKU:705195 - 1 eaAvailable on backorder
Cayman’s Malachite Green Phosphate Assay Kit provides a fast, reproducible, colorimetric method for measuring inorganic free phosphate in aqueous solutions. The assay method is based on the formation of a complex between malachite green molybdate and free orthophosphate that absorbs at 620-640 nm.{15225,15228} Applications for this assay include quantification of phosphorylation and phosphate release from protein phosphatase substrates.{15227} This assay is a reliable and suitable means of detecting and quantifying minimal amounts of inorganic free phosphate and is amenable to high-throughput screening applications.{15226} The assay is formatted to a 96-well format, but could easily be modified for use in 384-well or cuvette-based assays.
Brand:CaymanSKU:10009325 - 2 x 96 wellsAvailable on backorder
Microtubule-associated protein 2 (MAP2) belongs to the microtubule-associated protein (MAP) family of cytoskeletal filament proteins, which includes MAP1-5 and tau.{55112} MAP2 is composed of an N-terminal projection domain that interacts with PKA and a C-terminal domain that contains microtubule binding repeats containing a KXGS motif that is subject to phosphorylation.{55113} Five isoforms of MAP2 are generated through alternative splicing and are found in the cell body and dendrites of neurons, with some isoforms also found in astrocytes and oligodendrocytes of the CNS and in the testis.{55113,55112} MAP2 is expressed in mature neurons, with the MAP2a and MAP2c isoforms expressed in a developmental stage-dependent manner while the MAP2b isoform is expressed throughout development and adulthood.{55114,55112} MAP2 binds to and stabilizes microtubules and increases their rigidity, as well as interacts with F-actin during neurite initiation and with proteins involved in signal transduction.{55113} Phosphorylation of the KXGS motif by MAP/microtubule affinity-regulating kinase 1 (MARK1), MARK2, or PKA decreases the affinity of MAP2 for microtubules, and the detachment of MAP2 leads to microtubule destabilization and disassembly. Decreased levels of MAP2 protein have been found in postmortem brain tissue from patients with a Lewy body variant of Alzheimer’s disease.{55115} MAP2 has been found in cytoplasmic neuronal Lewy bodies colocalized with α-synuclein and ubiquitin in postmortem substantia nigra from patients with Parkinson’s disease.{55116} Cayman’s MAP2 (bovine) Monoclonal Antibody (Clone 4H5) can be used for immunocytochemistry (ICC), immunohistochemistry (IHC), and Western blot (WB) applications. The antibody recognizes MAP2 at approximately 280 kDa from human, bovine, mouse, and rat samples.
Brand:CaymanSKU:29280 - 100 µlAvailable on backorder
Microtubule-associated protein 2 (MAP2) belongs to the microtubule-associated protein (MAP) family of cytoskeletal filament proteins, which includes MAP1-5 and tau.{55112} MAP2 is composed of an N-terminal projection domain that interacts with PKA and a C-terminal domain that contains microtubule binding repeats containing a KXGS motif that is subject to phosphorylation.{55113} Five isoforms of MAP2 are generated through alternative splicing and are found in the cell body and dendrites of neurons, with some isoforms also found in astrocytes and oligodendrocytes of the CNS and in the testis.{55112,55113} MAP2 is expressed in mature neurons, with the MAP2a and MAP2c isoforms expressed in a developmental stage-dependent manner while the MAP2b isoform is expressed throughout development and adulthood.{55112,55114} MAP2 binds to and stabilizes microtubules and increases their rigidity, as well as interacts with F-actin during neurite initiation and with proteins involved in signal transduction.{55113} Phosphorylation of the KXGS motif by MAP/microtubule affinity-regulating kinase 1 (MARK1), MARK2, or PKA decreases the affinity of MAP2 for microtubules, and the detachment of MAP2 leads to microtubule destabilization and disassembly. Decreased levels of MAP2 protein have been found in postmortem brain tissue from patients with a Lewy body variant of Alzheimer’s disease.{55115} MAP2 has been found in cytoplasmic neuronal Lewy bodies colocalized with α-synuclein and ubiquitin in postmortem substantia nigra from patients with Parkinson’s disease.{55116} Cayman’s MAP2 (bovine) Polyclonal Antibody can be used for immunocytochemistry (ICC), immunohistochemistry (IHC), Western blot (WB) applications. The antibody recognizes MAP2 at approximately 280 kDa from human, bovine, mouse, and rat samples.
Brand:CaymanSKU:29281 - 100 µlAvailable on backorder
Microtubule-associated protein 2 (MAP2) belongs to the microtubule-associated protein (MAP) family of cytoskeletal filament proteins, which includes MAP1-5 and tau.{55112} MAP2 is composed of an N-terminal projection domain that interacts with PKA and a C-terminal domain that contains microtubule binding repeats containing a KXGS motif that is subject to phosphorylation.{55113} Five isoforms of MAP2 are generated through alternative splicing and are found in the cell body and dendrites of neurons, with some isoforms also found in astrocytes and oligodendrocytes of the CNS and in the testis.{55112,55113} MAP2 is expressed in mature neurons, with the MAP2a and MAP2c isoforms expressed in a developmental stage-dependent manner while the MAP2b isoform is expressed throughout development and adulthood.{55112,55114} MAP2 binds to and stabilizes microtubules and increases their rigidity, as well as interacts with F-actin during neurite initiation and with proteins involved in signal transduction.{55113} Phosphorylation of the KXGS motif by MAP/microtubule affinity-regulating kinase 1 (MARK1), MARK2, or PKA decreases the affinity of MAP2 for microtubules, and the detachment of MAP2 leads to microtubule destabilization and disassembly. Decreased levels of MAP2 protein have been found in postmortem brain tissue from patients with a Lewy body variant of Alzheimer’s disease.{55115} MAP2 has been found in cytoplasmic neuronal Lewy bodies colocalized with α-synuclein and ubiquitin in postmortem substantia nigra from patients with Parkinson’s disease.{55116} Cayman’s MAP2 (human) Monoclonal Antibody can be used for immunocytochemistry (ICC), immunohistochemistry (IHC), and Western blot (WB) applications. The antibody recognizes MAP2 at approximately 280 kDa from human, mouse, and rat samples.
Brand:CaymanSKU:29282 - 100 µlAvailable on backorder
Maresins (macrophage mediators in resolving inflammation) are members of a class of potent anti-inflammatory and pro-resolving mediators that are synthesized by macrophages in the presence of docosahexaenoic acid (DHA). Maresin 1 (MaR1) is produced via oxidation of endogenous DHA by 12-lipoxygenase. MaR1 has been shown to reduce neutrophil infiltration in murine peritonitis, enhance efferocytosis, inhibit TRPV1 currents in neurons, reduce neuropathic pain in mice, and attenuate disease severity in mouse models of colitis. Although circulating levels of MaR1 may be quite low, MaR1 can be detected in urine.
Brand:CaymanSKU:501150 - 96 solid wellsAvailable on backorder
Maresins (macrophage mediators in resolving inflammation) are members of a class of potent anti-inflammatory and pro-resolving mediators that are synthesized by macrophages in the presence of docosahexaenoic acid (DHA). Maresin 1 (MaR1) is produced via oxidation of endogenous DHA by 12-lipoxygenase. MaR1 has been shown to reduce neutrophil infiltration in murine peritonitis, enhance efferocytosis, inhibit TRPV1 currents in neurons, reduce neuropathic pain in mice, and attenuate disease severity in mouse models of colitis. Although circulating levels of MaR1 may be quite low, MaR1 can be detected in urine.
Brand:CaymanSKU:501150 - 96 strip wellsAvailable on backorder
MAT1 also known as MNAT1 belong to the complex of Cyclin dependent kinase-activating kinase, together with CDK-7 and Cyclin H where it plays he key role for regulating the activity of the complex. It is also playing a significant role in cell cycle, transcription and differentiation. [Bertin Catalog No. G01019]
Brand:CaymanSKU:32788 - 100 µlAvailable on backorder
MAT1 also known as MNAT1 belong to the complex of Cyclin dependent kinase-activating kinase, together with CDK-7 and Cyclin H where it plays the key role for regulating the activity of the complex. It also plays a significant role in cell cycle, transcription, and differentiation. [Bertin Catalog No. G01020]
Brand:CaymanSKU:32789 - 100 µlAvailable on backorder
Mitochondrial antiviral-signaling protein (MAVS) is a mitochondrial membrane-bound adapter protein that initiates antiviral immunity.{46479} MAVS is activated upon viral infection by the dsRNA-sensing receptors RIG-I and MDA5 and by mitochondrial reactive oxygen species (ROS). Upon activation, MAVS polymerizes and binds to TANK-binding kinase 1 (TBK1) and IKKε, which form a signaling platform to activate IFN regulatory factor 3 (IRF3) and NF-ĸB, respectively.{46479,46480} MAVS also mediates recruitment of the NLRP3 inflammasome to the mitochondria and activates caspase-1 to release IL-1β in response to non-crystalline inflammasome activators.{46481} Embryonic fibroblasts, peritoneal and bone marrow-derived macrophages (BMDMs), and conventional, but not plasmacytoid, dendritic cells from Mavs-/- mice exhibit reduced Sendai virus-induced increases in IFN-α and IFN-β secretion.{46482} Serum viral titers are increased and survival is decreased in Mavs-/- mice infected with vesicular stomatitis virus compared with wild-type controls. MAVS-deficient, SLE-prone Fcgr2b-/- mice have decreased serum levels of antinuclear autoantibodies and increased survival compared with MAVS-expressing Fcgr2b-/- mice.{46484} Aggregates of MAVS and plasma levels of IFN-β and the autoantibodies Sm and UN1RNP are increased in peripheral blood mononuclear cells (PBMCs) isolated from patients with systemic lupus erythematosus (SLE).{46483} Cayman’s MAVS Monoclonal Antibody (Clone 7B9) can be used for ELISA, Immunohistochemistry, and Western blot applications. The antibody recognizes MAVS at 56.5 kDa from human samples.
Brand:CaymanSKU:26355 - 100 µgAvailable on backorder
Membrane bound O-acyl transferases (MBOATs) are a group of conserved, multiple transmembrane spanning enzymes involved in many biological functions including lipid biosynthesis, embryogenesis, nutrient sensing, and membrane lipid remodeling. This group of proteins is associated with pathologies such as diabetes, obesity, atherosclerosis, and Alzheimer’s disease.{27302} MBOAT1 functions as an acyltransferase which mediates the conversion of lysophosphatidylserine (1-acyl-2-hydroxy-sn-glycero-3-phospho-L-serine or LPS) into phosphatidylserine (1,2-diacyl-sn-glycero-3-phospho-L-serine or PS) (LPSAT activity), and prefers oleoyl-CoA as the acyl donor. Lysophospholipid acyltransferases catalyze the reacylation step of the phospholipid remodeling pathway also known as the Lands cycle.{27123} The predicted size of MBOAT1 is 56.6 kDa, and Cayman’s MBOAT1 Polyclonal Antibody detects a band at approximately 60 kDa by western blot.
Brand:CaymanSKU:14698 - 1 eaAvailable on backorder