Description
Boster’s mouse CD300b ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD300b has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: I18-P209) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD300b is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD300b amount of sample captured in plate.
Synonyms: CMRF35-like molecule 7; CLM-7; CD300 antigen-like family member B; Immune receptor expressed on myeloid cells 3; IREM-3; Leukocyte mono-Ig-like receptor 5; CD300b; Cd300lb; Irem3; Lmir5
Species: Mouse CD300b
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse CD300b
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, I18-P209
Gene name: CMRF35-like molecule 7 / CD300 molecule like family member B
Protein name: CMRF35-like molecule 7
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|19|70|278|23|118|418
Standard deviation|0.78|3.29|13.62|1.17|6.49|26.33
CV(%)|4.1%|4.7%|4.9%|5.1%|5.5%|6.3%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 19 | 21 | 21 | 19 | 20 | 1.00 | 5.0% |
| Sample 2 | 70 | 67 | 57 | 66 | 65 | 4.84 | 7.4% |
| Sample 3 | 278 | 270 | 278 | 255 | 270 | 9.39 | 3.4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1865-CAP|96-well plate precoated with anti- mouse CD300b antibody|1
EK1865-ST|Lyophilized recombinant mouse CD300b standard|10ng/tube×2
EK1865-DA|Biotinylated anti- mouse CD300b antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
