Description
Boster’s human Nesfatin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Nesfatin-1 has been precoated onto 96-well plates. Standards(E.coli, V25-L106) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Nesfatin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Nesfatin-1 amount of sample captured in plate.
Synonyms: DNA binding protein NEFA|DNA-binding protein NEFA|Gastric cancer antigen Zg4|NEFA|Nefastin 1|Nefastin-1|Nefastin1|Nucb2|NUCB2_HUMAN|Nucleobindin-2|Nucleobinding 2|Prepronefastin|Prepronesfatin
Species: human Nesfatin-1
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse CCL8
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: E.coli, V25-L106
Gene name: Nucleobindin-2 / nucleobindin 2
Protein name: Nucleobindin-2
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|58|278|993|62|286|1045
Standard deviation|2.55|17.51|55.6|3.41|20.59|74.19
CV(%)|4.4%|6.3%|5.6%|5.5%|7.2%|7.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 58 | 50 | 49 | 55 | 53 | 3.67 | 6.9% |
| Sample 2 | 278 | 249 | 272 | 250 | 262 | 12.93 | 4.9% |
| Sample 3 | 993 | 1004 | 914 | 846 | 939 | 64.06 | 6.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1138-CAP|96-well plate precoated with anti- human Nesfatin-1 antibody|1
EK1138-ST|Lyophilized recombinant human Nesfatin-1 standard|2ng/tube×2
EK1138-DA|Biotinylated anti- human Nesfatin-1 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
