Description
Boster’s human TrkA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TrkA has been precoated onto 96-well plates. Standards (NSO,A33-E407) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TrkA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TrkA amount of sample captured in plate.
Synonyms: High affinity nerve growth factor receptor|_x000D_MTC|_x000D_Neurotrophic tyrosine kinase receptor type 1|_x000D__x000D__x000D_NTRK1|_x000D_NTRK1_HUMAN|_x000D_Oncogene TRK|_x000D_p140-TrkA|_x000D_TRK|_x000D_Trk-A|_x000D_TRK1|_x000D_TRK1-transforming tyrosine kinase protein
Species: Human TrkA
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human TrkA
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: NSO,A33-E407
Gene name: High affinity nerve growth factor receptor / neurotrophic tyrosine kinase, receptor, type 1
Protein name: High affinity nerve growth factor receptor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|314|1718|5724|303|1722|6073
Standard deviation|22.29|92.77|360.61|25.45|117.09|406.89
CV(%)|7.1%|5.4%|6.3%|8.4%|6.8%|6.7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 314 | 293 | 280 | 296 | 295 | 12.13 | 4.1% |
| Sample 2 | 1718 | 1671 | 1601 | 1714 | 1676 | 47.05 | 2.8% |
| Sample 3 | 5724 | 5182 | 5771 | 4794 | 5367 | 404.1 | 7.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0846-CAP|96-well plate precoated with anti- human TrkA antibody|1
EK0846-ST|Lyophilized recombinant human TrkA standard|10ng/tube×2
EK0846-DA|Biotinylated anti- human TrkA antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
