Description
Boster’s mouse MBL2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MBL2 has been precoated onto 96-well plates. Standards(NSO, E19-D244) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MBL2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MBL2 amount of sample captured in plate.
Synonyms: COLEC 1|COLEC1|Collectin-1|HSMBPC|Lectin, mannose-binding, soluble, 2|Mannan binding lectin|Mannan binding protein|Mannan-binding protein|Mannose binding lectin (protein C) 2 soluble|Mannose binding lectin (protein C) 2, soluble (opsonic defect)|Mannose binding lectin (protein C) 2, soluble|Mannose binding lectin 2 soluble|Mannose binding lectin 2, soluble (opsonic defect)|Mannose binding lectin|Mannose binding lectin protein C2 soluble opsonic defect|Mannose binding protein|Mannose binding protein C|Mannose binding protein C precursor|Mannose binding protein, serum|Mannose-binding lectin|Mannose-binding protein C|MBL 2|MBL|MBL2|MBL2_HUMAN|MBL2D|MBP 1|MBP|MBP C|MBP-C|MBP1|MBPB|MBPC|MBPD|MGC116832|MGC116833|Opsonic defect|protein C|Soluble mannose binding lectin
Species: Mouse MBL2
Assay: 312pg/ml-20000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse MBL2
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, E19-D244
Gene name: Mannose-binding protein C / mannose-binding lectin (protein C) 2, soluble
Protein name: Mannose-binding protein C
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|656|1840|9232|637|1954|8997
Standard deviation|32.8|84.64|618.54|42.04|111.37|665.77
CV(%)|5%|4.6%|6.7%|6.6%|5.7%|7.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 656 | 711 | 654 | 663 | 671 | 23.33 | 3.4% |
| Sample 2 | 1840 | 1861 | 1847 | 1853 | 1850 | 7.72 | 0.4% |
| Sample 3 | 9232 | 10414 | 9072 | 9277 | 9498 | 533.88 | 5.6% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0806-CAP|96-well plate precoated with anti- mouse MBL2 antibody|1
EK0806-ST|Lyophilized recombinant mouse MBL2 standard|20ng/tube×2
EK0806-DA|Biotinylated anti- mouse MBL2 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
