Description
Boster’s human IGF1R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGF1R has been precoated onto 96-well plates. Standards(NSO, E31-N932) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF1R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGF1R amount of sample captured in plate.
Synonyms: CD221|IGF 1R|IGF I receptor|IGF-I receptor|Igf1r|IGF1R_HUMAN|IGFIR|IGFR|Insulin like growth factor 1 receptor|Insulin like growth factor I receptor|Insulin-like growth factor 1 receptor beta chain|Insulin-like growth factor I receptor|JTK13|MGC142170|MGC142172|MGC18216
Species: Human IGF1R
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human IGF1R
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, E31-N932
Gene name: Insulin-like growth factor 1 receptor / insulin-like growth factor 1 receptor
Protein name: Insulin-like growth factor 1 receptor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|332|1874|5843|363|1924|5918
Standard deviation|17.92|89.95|438.22|25.41|121.21|526.7
CV(%)|5.4%|4.8%|7.5%|7%|6.3%|8.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 332 | 335 | 290 | 335 | 323 | 19.09 | 5.9% |
| Sample 2 | 1874 | 1833 | 1855 | 2088 | 1912 | 102.35 | 5.3% |
| Sample 3 | 5843 | 5933 | 6248 | 5526 | 5887 | 257.24 | 4.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0776-CAP|96-well plate precoated with anti- human IGF1R antibody|1
EK0776-ST|Lyophilized recombinant human IGF1R standard|10ng/tube×2
EK0776-DA|Biotinylated anti- human IGF1Rantibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
