Description
Boster’s human CD25 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD25 has been precoated onto 96-well plates. Standards (NSO,E22-C213) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD25 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD25 amount of sample captured in plate.
Synonyms: CD122|CD122 antigen|CD132|CD132 antigen|CD25|CD25 antigen|Cytokine receptor common gamma chain|Gamma C|High affinity IL 2 receptor subunit beta|IDDM10|IL 2 RA|IL 2 receptor|IL 2 receptor alpha subunit|IL 2R gamma chain|IL2 RA|IL2R|IL2RA|IL2RB|IL2RG|IMD4|interleukin 2 receptor alpha|Interleukin 2 receptor alpha chain|interleukin 2 receptor beta|Interleukin 2 receptor beta chain|interleukin 2 receptor gamma|Interleukin 2 receptor gamma (severe combined immunodeficiency)|Interleukin 2 receptor gamma chain|p55|p64|P70 75|p75|SCIDX|SCIDX1|TAC antigen|TCGFR|XSCID
Species: Human CD25
Assay: 78pg/ml-5000pg/ml
Sensitivy: <5pg/ml Specifity: Natural and recombinant human CD25
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO,E22-C213
Gene name: Interleukin-2 receptor subunit alpha / interleukin 2 receptor, alpha
Protein name: Interleukin-2 receptor subunit alpha
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|140|719|2353|141|785|2310
Standard deviation|6.58|41.7|94.12|7.61|47.1|97.02
CV(%)|4.7%|5.8%|4%|5.4%|6%|4.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 140 | 116 | 139 | 115 | 127 | 12.01 | 9.4% |
| Sample 2 | 719 | 735 | 616 | 709 | 694 | 46.4 | 6.6% |
| Sample 3 | 2353 | 2484 | 2499 | 2400 | 2434 | 60.08 | 2.4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0400-CAP|96-well plate precoated with anti- human CD25 antibody|1
EK0400-ST|Lyophilized recombinant human CD25 standard|10ng/tubex2
EK0400-DA|Biotinylated anti- human CD25 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
