Description
Boster’s human IGFBP-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP-3 has been precoated onto 96-well plates. Standards(NSO, A28Q-291) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP-3 amount of sample captured in plate.
Synonyms: Acid stable subunit of the 140 K IGF complex|Binding protein 29|Binding protein 53|BP 53|BP53|Growth hormone dependent binding protein|IBP 3|IBP3|IGF binding protein 3|IGFBP 3|Insulin Like Growth Factor Binding Protein 3
Species: Human IGFBP-3
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human IGFBP-3
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, A28Q-291
Gene name: Insulin-like growth factor-binding protein 3 / insulin-like growth factor binding protein 3
Protein name: Insulin-like growth factor-binding protein 3
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|271|1607|5480|253|1639|5548
Standard deviation|16.53|69.1|350.72|19.73|95.06|438.29
CV(%)|6.1%|4.3%|6.4%|7.8%|5.8%|7.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 271 | 288 | 290 | 253 | 275 | 14.94 | 5.4% |
| Sample 2 | 1607 | 1578 | 1461 | 1560 | 1551 | 54.87 | 3.5% |
| Sample 3 | 5480 | 5896 | 5868 | 5913 | 5789 | 179.26 | 3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0386-CAP|96-well plate precoated with anti- human IGFBP-3 antibody|1
EK0386-ST|Lyophilized recombinant human IGFBP-3 standard|10ng/tubex2
EK0386-DA|Biotinylated anti- human IGFBP-3 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
