Description
Boster’s rat TGF beta 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGF beta 1 has been precoated onto 96-well plates. Standards(CHO, A279-S390) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF beta 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat TGF beta 1 amount of sample captured in plate.
Synonyms: Camurati-Engelmann disease|_x000D_CED|_x000D_DPD1|_x000D__x000D__x000D_LAP|_x000D_Latency-associated peptide|_x000D_TGF beta 1 protein|_x000D_TGF-beta-1|_x000D_TGFB|_x000D_tgfb1|_x000D_TGFB1_HUMAN|_x000D_TGFbeta|_x000D_Transforming growth factor beta 1
Species: Rat TGF beta 1
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant rat TGF beta 1
Cross Reactivity: There is cross-reactivates with TGFbeta2, TGFbeta3, TGFbeta5<1%. Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: EDTA
Immunogen: CHO, A279-S390
Gene name: Transforming growth factor beta-1 / transforming growth factor, beta 1
Protein name: Transforming growth factor beta-1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|27|161|431|28|156|422
Standard deviation|1.59|9.82|23.7|1.73|9.98|26.16
CV(%)|5.9%|6.1%|5.5%|6.2%|6.4%|6.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 27 | 31 | 29 | 32 | 29 | 1.92 | 6.6% |
| Sample 2 | 161 | 143 | 162 | 145 | 152 | 8.78 | 5.7% |
| Sample 3 | 431 | 413 | 424 | 455 | 430 | 15.4 | 3.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0514-CAP|96-well plate precoated with anti- rat TGF beta 1 antibody|1
EK0514-ST|Lyophilized recombinant rat TGF beta 1 standard|10ng/tubex2
EK0514-DA|Biotinylated anti- rat TGF beta 1 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
