Description
Boster’s rat SEMA3F ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SEMA3F has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: A19-T785) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SEMA3F is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substmousee TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Synonyms: Plexin-A3; Plxna3
Species: Rat SEMA3F
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant rat SEMA3F
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: NSO, A19-T785
Gene name: Plexin-A3 / semaphorin 3F
Protein name: Plexin-A3
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|58|227|919|59|236|947
Standard deviation|3.02|12.03|43.19|3.60|17.46|48.30
CV(%)|5.2%|5.3%|4.7%|6.1%|7.4%|5.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 58 | 54 | 55 | 54 | 55 | 1.63 | 2.9% |
| Sample 2 | 227 | 223 | 217 | 225 | 223 | 3.74 | 1.6% |
| Sample 3 | 919 | 937 | 964 | 972 | 948 | 21.17 | 2.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1735-CAP|96-well plate precoated with anti- rat SEMA3F antibody|1
EK1735-ST|Lyophilized recombinant rat SEMA3F standard|10ng/tube×2
EK1735-DA|Biotinylated anti- rat SEMA3F antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
