Description
Boster’s rat NGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NGF has been precoated onto 96-well plates. Standards(NSO, S122-G241) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat NGF amount of sample captured in plate.
Synonyms: Beta nerve growth factor|Beta NGF|HSAN5|MGC161426|MGC161428|Nerve growth factor (beta polypeptide)|Nerve growth factor beta polypeptide|Nerve growth factor beta subunit|NGFB|NID67
Species: Rat NGF
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant rat NGF
Cross Reactivity: There is no detectable cross-reactivity with BDNF, GDNF, CNTF, NT3, and NT4.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: NSO, S122-G241
Gene name: Beta-nerve growth factor / nerve growth factor (beta polypeptide)
Protein name: Beta-nerve growth factor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|33|191|480|29|201|491
Standard deviation|1.84|8.02|32.64|1.62|11.65|35.84
CV(%)|5.6%|4.2%|6.8%|5.6%|5.8%|7.3%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 33 | 32 | 33 | 35 | 33 | 1.08 | 3.2% |
| Sample 2 | 191 | 172 | 185 | 192 | 185 | 7.96 | 4.3% |
| Sample 3 | 480 | 451 | 440 | 436 | 451 | 17.21 | 3.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0471-CAP|96-well plate precoated with anti- rat NGF antibody|1
EK0471-ST|Lyophilized recombinant rat NGF standard|10ng/tubex2
EK0471-DA|Biotinylated anti- rat NGF antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
