Description
Boster’s rat MCP-1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MCP-1 has been precoated onto 96-well plates. Standards(NSO, Q24-N148) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MCP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat MCP-1 amount of sample captured in plate.
Synonyms: C-C motif chemokine 2|CCL2|CCL2_HUMAN|Chemokine (C-C motif) ligand 2|GDCF-2|HC11|HSMCR30|MCAF|MCP-1|MCP1|MGC9434|Monocyte chemoattractant protein 1|Monocyte chemoattractant protein-1|Monocyte chemotactic and activating factor|Monocyte chemotactic protein 1|Monocyte secretory protein JE|SCYA2|Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)|Small inducible cytokine subfamily A (Cys-Cys), member 2|Small-inducible cytokine A2|SMC-CF
Species: Rat MCP-1
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant rat MCP-1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: NSO, Q24-N148
Gene name: C-C motif chemokine 2 / chemokine (C-C motif) ligand 2
Protein name: C-C motif chemokine 2
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|41|257|473|40|251|504
Standard deviation|2.29|18.5|27.43|2.56|21.58|29.23
CV(%)|5.6%|7.2%|5.8%|6.4%|8.6%|5.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 41 | 37 | 39 | 42 | 39 | 1.92 | 4.9% |
| Sample 2 | 257 | 231 | 216 | 228 | 233 | 14.94 | 6.4% |
| Sample 3 | 473 | 462 | 446 | 461 | 460 | 9.6 | 2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0902-CAP|96-well plate precoated with anti- rat MCP-1 antibody|1
EK0902-ST|Lyophilized recombinant rat MCP-1 standard|10ng/tube×2
EK0902-DA|Biotinylated anti- rat MCP-1 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
