Description
Boster’s rat KIM1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KIM1 has been precoated onto 96-well plates. Standards(NSO, S18-V238) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KIM1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat KIM1 amount of sample captured in plate.
Synonyms: HAVCR|_x000D_HAVcr-1|_x000D_Havcr1|_x000D__x000D__x000D_HAVR1_HUMAN|_x000D_Hepatitis A virus cellular receptor 1|_x000D_Kidney injury molecule 1|_x000D_KIM-1|_x000D_T cell immunoglobin domain and mucin domain protein 1|_x000D_T-cell immunoglobulin and mucin domain-containing protein 1|_x000D_T-cell membrane protein 1|_x000D_TIM|_x000D_TIM-1|_x000D_TIM1|_x000D_TIMD-1|_x000D_TIMD1
Species: Rat KIM1
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <2pg/ml Specifity: Natural and recombinant rat KIM1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, S18-V238
Gene name: Hepatitis A virus cellular receptor 1 / hepatitis A virus cellular receptor 1
Protein name: Hepatitis A virus cellular receptor 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|87|272|938|86|293|1026
Standard deviation|3.82|20.67|59.09|3.95|23.14|84.13
CV(%)|4.4%|7.6%|6.3%|4.6%|7.9%|8.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 87 | 94 | 102 | 93 | 94 | 5.33 | 5.6% |
| Sample 2 | 272 | 238 | 277 | 253 | 260 | 15.54 | 5.9% |
| Sample 3 | 938 | 849 | 928 | 895 | 902 | 34.74 | 3.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0882-CAP|96-well plate precoated with anti- rat KIM1 antibody|1
EK0882-ST|Lyophilized recombinant rat KIM1 standard|10ng/tube×2
EK0882-DA|Biotinylated anti- rat KIM1 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
