Description
Boster’s rat IL-1 alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1 alpha has been precoated onto 96-well plates. Standards (E.coli,S115-S270) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-1 alpha amount of sample captured in plate.
Synonyms: Hematopoietin 1|_x000D_IFL|_x000D_IFN|_x000D__x000D__x000D_IFN alpha|_x000D_IFN-alpha-1/13|_x000D_IFNA@|_x000D_IFNA1|_x000D_IFNA1_HUMAN|_x000D_IFNA13|_x000D_IFNAP22|_x000D_IL 1 alpha|_x000D_Il 1a|_x000D_IL1|_x000D_IL1 ALPHA|_x000D_IL1A|_x000D_IL1F1|_x000D_Ilia|_x000D_Interferon Alpha|_x000D_Interferon alpha 1|_x000D_Interferon alpha 1/13|_x000D_Interferon alpha 1/13 precursor|_x000D_Interferon alpha 13|_x000D_Interferon alpha D|_x000D_Interferon alpha leukocyte|_x000D_Interferon alpha-1/13|_x000D_Interferon alpha-D|_x000D_Interferon leukocyte|_x000D_Interleukin 1 alpha|_x000D_Interleukin 1 alpha precursor|_x000D_Interleukin1 alpha|_x000D_LeIF D|_x000D_MGC138207|_x000D_MGC138505|_x000D_MGC138507|_x000D_OTTHUMP00000203692|_x000D_OTTMUSP00000016430|_x000D_Preinterleukin 1|_x000D_Pro interleukin 1|_x000D_Pro interleukin 1 alpha|_x000D_Prointerleukin 1|_x000D_RP23-160G19.8
Species: Rat IL-1 alpha
Assay: 4.69pg/ml-300pg/ml
Sensitivy: <0.5pg/ml Specifity: Natural and recombinant rat IL-1 alpha
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: E.coli,S115-S270
Gene name: Interleukin-1 alpha / interleukin 1, alpha
Protein name: Interleukin-1 alpha
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|15|40|131|14|43|120
Standard deviation|1.06|2.12|9.69|1.09|2.58|10.8
CV(%)|7.1%|5.3%|7.4%|7.8%|6%|9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 15 | 16 | 15 | 17 | 15 | 0.82 | 5.4% |
| Sample 2 | 40 | 39 | 46 | 45 | 42 | 3.04 | 7.2% |
| Sample 3 | 131 | 123 | 146 | 136 | 134 | 8.33 | 6.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0390-CAP|96-well plate precoated with anti- rat IL-1 alpha antibody|1
EK0390-ST|Lyophilized recombinant rat IL-1 alpha standard|10ng/tubex2
EK0390-DA|Biotinylated anti- rat IL-1 alpha antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
