Description
Boster’s rat Endothelin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Endothelin has been precoated onto 96-well plates. Standards (rat peptide,C53-W73) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Endothelin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Endothelin amount of sample captured in plate.
Synonyms: Big endothelin 1|ET1|HDLCQ7|PPET1|Preproendothelin 1|EDN2|EDN2_HUMAN|Endothelin 2|Endothelin-2|ET 2|ET-2|ET2|PPET2|Preproendothelin 2|Preproendothelin-2|EDN3|EDN3_HUMAN|Endothelin-3|ET 3|ET-3|PPET3|Preproendothelin 3|Preproendothelin-3
Species: Rat Endothelin
Assay: 3.9pg/ml-250pg/ml
Sensitivy: <0.5pg/ml Specifity: Natural and recombinant rat Endothelin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated monoclonal antibody from mouse
Anticoagulant: heparin or EDTA
Immunogen: human peptide, C53-W73
Gene name: Endothelin-1 / endothelin 1
Protein name: Endothelin-1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|13|57|113|12|56|105
Standard deviation|0.78|4.21|5.19|0.94|4.48|6.4
CV(%)|6%|7.4%|4.6%|7.9%|8%|6.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 13 | 14 | 12 | 12 | 12 | 0.82 | 6.8% |
| Sample 2 | 57 | 55 | 60 | 52 | 56 | 2.91 | 5.1% |
| Sample 3 | 113 | 120 | 129 | 118 | 120 | 5.78 | 4.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0952-CAP|96-well plate precoated with anti- rat Endothelin antibody|1
EK0952-ST|Lyophilized recombinant rat Endothelin standard|1ng/tube×2
EK0952-DA|Biotinylated anti- rat Endothelin antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
