Description
Boster’s rat CXCL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL1 has been precoated onto 96-well plates. Standards(E. coli, A25-K96) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CXCL1 amount of sample captured in plate.
Synonyms: C-X-C motif chemokine 1|chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)|fibroblast secretory protein|FSP|GRO|GRO protein, alpha|GRO-alpha(1-73)|GRO1|GRO1 oncogene|GRO1 oncogene (melanoma growth stimulating activity, alpha)|GRO1 oncogene (melanoma growth-stimulating activity)|GROa|growth-regulated alpha protein|Keratinocyte-derived chemokine, mouse, homolog of|Melanoma growth stimulatory activity|melanoma growth stimulatory activity alpha|MGSA|MGSA a|MGSA alpha|NAP 3|Neutrophil-activating protein 3|SCYB1|Small inducible cytokine subfamily B, member 1
Species: Rat CXCL1
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant rat CXCL1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: EDTA
Immunogen: E.coli, A25-K96
Gene name: Growth-regulated alpha protein / chemokine (C-X-C motif) ligand 1
Protein name: Growth-regulated alpha protein
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|36|226|428|36|217|466
Standard deviation|1.58|9.26|26.1|1.58|9.33|33.55
CV(%)|4.4%|4.1%|6.1%|4.4%|4.3%|7.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 36 | 37 | 34 | 32 | 34 | 1.92 | 5.6% |
| Sample 2 | 226 | 219 | 236 | 214 | 223 | 8.25 | 3.6% |
| Sample 3 | 428 | 378 | 438 | 405 | 412 | 23.11 | 5.6% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0724-CAP|96-well plate precoated with anti- rat CXCL1 antibody|1
EK0724-ST|Lyophilized recombinant rat CXCL1 standard|10ng/tube×2
EK0724-DA|Biotinylated anti- rat CXCL1 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
