Description
Boster’s rat Activin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Activin A has been precoated onto 96-well plates. Standards(CHO, G311-S426) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Activin A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Activin A amount of sample captured in plate.
Synonyms: Activin beta A chain|erythroid differentiation factor|erythroid differentiation protein|follicle stimulating hormone releasing protein|FSH releasing protein|Inhibin|inhibin beta A chain
Species: Rat Activin A
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <12pg/ml Specifity: Natural and recombinant rat Activin A
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated monoclonal antibody from mouse
Anticoagulant: heparin or EDTA
Immunogen: CHO, G311-S426
Gene name: Inhibin beta A chain / inhibin, beta A
Protein name: Inhibin beta A chain
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|31|151|450|28|138|453
Standard deviation|1.3|8|25.2|1.56|8.69|25.36
CV(%)|4.2%|5.3%|5.6%|5.6%|6.3%|5.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 31 | 31 | 27 | 31 | 30 | 1.73 | 5.7% |
| Sample 2 | 151 | 165 | 144 | 171 | 157 | 10.75 | 6.8% |
| Sample 3 | 450 | 412 | 404 | 462 | 432 | 24.53 | 5.6% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0303-CAP|96-well plate precoated with anti- rat Activin A antibody|1
EK0303-ST|Lyophilized recombinant rat Activin A standard|10ng/tube×2
EK0303-DA|Biotinylated anti- rat Activin A antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
