Immunogen: Full-length recombinant PAD6 protein • Host: Mouse • Cross reactivity: (-) PAD1

PAD6 Monoclonal Antibody (Clone 4B7) – 100 µg

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Cayman
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Protein arginine deiminase 6 (PAD6) is a homodimeric guanidine-modifying enzyme belonging to the amidinotransferase superfamily.{34555} It is a calcium-dependent enzyme that catalyzes the post-translational modification of target proteins by converting arginine to citrulline. PAD6 is expressed in mammalian oocytes, sperm cells, and early embryos.{39678} In mammalian oocytes and early embryo cytoplasm, its expression is localized to cytoskeletal sheets, dynamic structures containing various keratins, which are major targets for citrullination. PAD6-/- oocytes exhibit reduced microtubule acetylation and defective organelle positioning and redistribution, suggesting a role for PAD6 in regulating microtubule-mediated organelle movement and positioning.{39677} PAD6-/- female, but not male, mice are infertile due to a reduction of de novo protein synthesis, cytoskeletal sheet formation, and ribosomal RNA which induces arrest of zygote development at the two-cell stage.{39678,39679} PAD6 is regulated by newborn ovary homeobox (Nobox), as its promoter contains a Nobox DNA-binding element (NBE) and expression and activity of PAD6 are decreased in Nobox-/- mouse ovaries.{39676} In human females, homozygous nonsense mutations and compound-heterozygous mutations in PAD6 induce early embryonic arrest following in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI).{39680} Cayman’s PAD6 monoclonal antibody (Clone 4B7) can be used for Western blot and ELISA applications. The antibody recognizes PAD6 at ~77 kDa from human samples.  

 

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SKU: 25965 - 100 µg Category:

Description

Immunogen: Full-length recombinant PAD6 protein • Host: Mouse • Cross reactivity: (-) PAD1, PAD2, PAD3, PAD4 • Species Reactivity: (+) Human PAD6 • Isotype: IgG1 • Applications: ELISA and WB • MW = ~77 kDa

Synonyms: hPADVI|PADI6|Peptidylarginine Deiminase 6|Protein Arginine Deiminase 6

Immunogen: Full-length human recombinant PAD6 protein

Formulation: 100 µg of protein G-purified monoclonal antibody

Isotype: IgG1

Applications: ELISA and WB

Origin:

Stability: 365 days

Application|ELISA||Application|Western Blot||Product Type|Antibodies|Monoclonal Antibodies||Research Area|Cell Biology|Cytoskeleton & Motor Proteins||Research Area|Endocrinology & Metabolism|Reproductive Biology

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