The NF-κB/Rel family of transcription factors is comprised of several structurally-related proteins that form homodimers and heterodimers and include p50/p105

NF-κB (p65) Transcription Factor Assay Kit – 96 wells

Brand:
Cayman
Storage:
-80

Cayman’s NF-κB (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-κB response element is immobilized to the wells of a 96-well plate. NF-κB contained in a nuclear extract, binds specifically to the NF-κB response element. NF-κB (p65) is detected by addition of specific primary antibody directed against NF-κB (p65). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. Cayman’s NF-κB (p65) Transcription Factor Assay detects human NF-κB (p65). It will not cross-react with NF-κB (p50).  

 

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SKU: 10007889 - 96 wells Category:

Description

The NF-κB/Rel family of transcription factors is comprised of several structurally-related proteins that form homodimers and heterodimers and include p50/p105, p52/p100, RelA (p65), and c-Rel/NF-κB. Acting as dimers, these transcription factors bind to κB sites on DNA, thereby regulating expression of target genes. The most common Rel/NF-κB dimer in mammals contains p50-RelA (p50/p65) heterodimers and is an attractive target for potential therapeutics in human inflammation and certain other diseases. Cayman’s NF-κB (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting human NF-κB (p65) DNA binding activity in nuclear extracts and whole cell lysates. It will not cross-react with NF-κB (p50).

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Product Type|Assay Kits|ELISA||Research Area|Immunology & Inflammation|Autoimmunity||Research Area|Immunology & Inflammation|Innate Immunity|Pattern Recognition||Research Area|Immunology & Inflammation|Innate Immunity|STING||Research Area|Infectious Disease

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