Description
Boster’s mouse Vitamin DBP ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Vitamin DBP has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: L17-S476) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Vitamin DBP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Vitamin DBP amount of sample captured in plate.
Synonyms: Vitamin D-binding protein; DBP; VDB; Gc protein-derived macrophage activating factor; Gc-MAF; GcMAF; Gc-globulin; Group-specific component; Gc; Vitamin D-binding protein-macrophage activating factor; DBP-maf; GC
Species: Mouse Vitamin DBP
Assay: 156pg/ml-10000pg/ml
Sensitivy: <50pg/ml Specifity: Natural and recombinant mouse Vitamin DBP
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin
Immunogen: NSO, L17-S476
Gene name: Vitamin D-binding protein / GC, vitamin D binding protein
Protein name: Vitamin D-binding protein
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|268|970|3393|309|1028|4064
Standard deviation|10.99|41.71|156.08|17.92|70.93|292.61
CV(%)|4.1%|4.3%|4.6%|5.8%|6.9%|7.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 268 | 284 | 253 | 255 | 265 | 12.38 | 4.6% |
| Sample 2 | 970 | 965 | 981 | 1065 | 995 | 40.68 | 4.0% |
| Sample 3 | 3393 | 3500 | 3134 | 3533 | 3390 | 156.59 | 4.6% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK2063-CAP|96-well plate precoated with anti- mouse Vitamin DBP antibody|1
EK2063-ST|Lyophilized recombinant mouse Vitamin DBP standard|10ng/tube×2
EK2063-DA|Biotinylated anti- mouse Vitamin DBP antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
