Description
Boster’s mouse VEGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for VEGF has been precoated onto 96-well plates. Standards (sf21,A27-R190) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse VEGF amount of sample captured in plate.
Synonyms: Vascular endothelial growth factor A;VEGF-A;Vascular permeability factor;VPF;Vegfa;Vegf;
Species: Mouse VEGF
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <2pg/ml Specifity: This kit recognizes both the 164 and 120 amino acid residue forms of mouse VEGF
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: monoclonal antibody from rat|polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: Expression system for standard: sf21,A27-R190
Gene name: Vascular endothelial growth factor A / Vascular endothelial growth factor A
Protein name: Vascular endothelial growth factor A
Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 40 | 179 | 538 | 40 | 171 | 574 |
| Standard deviation | 1.92 | 9.48 | 36.58 | 2.6 | 9.57 | 48.79 |
| CV(%) | 4.8% | 5.3% | 6.8% | 6.5% | 5.6% | 8.5% |
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 40 | 34 | 35 | 37 | 36 | 2.29 | 6.3% |
| Sample 2 | 179 | 204 | 186 | 197 | 191 | 9.65 | 5% |
| Sample 3 | 538 | 463 | 494 | 471 | 491 | 29.15 | 5.9% |
*number of samples for each test n=16.
10ng/tube
