Description
Boster’s mouse TFPI ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TFPI has been precoated onto 96-well plates. Standards(NSO, L29-N306) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TFPI is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TFPI amount of sample captured in plate.
Synonyms: EPI|_x000D_Extrinsic pathway inhibitor|_x000D_LACI|_x000D__x000D__x000D_Lipoprotein-associated coagulation inhibitor|_x000D_TFPI|_x000D_TFPI1_HUMAN|_x000D_Tissue factor pathway inhibitor
Species: mouse TFPI
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse TFPI
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, L29-N306
Gene name: Tissue factor pathway inhibitor / tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
Protein name: Tissue factor pathway inhibitor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|310|1506|4359|330|1630|4238
Standard deviation|20.15|99.39|217.95|22.44|123.88|216.13
CV(%)|6.5%|6.6%|5%|6.8%|7.6%|5.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 310 | 324 | 315 | 312 | 315 | 5.35 | 1.6% |
| Sample 2 | 1506 | 1410 | 1529 | 1285 | 1432 | 96.14 | 6.7% |
| Sample 3 | 4359 | 4343 | 4246 | 3938 | 4221 | 169.29 | 4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1355-CAP|96-well plate precoated with anti- mouse TFPI antibody|1
EK1355-ST|Lyophilized recombinant mouse TFPI standard|10ng/tube×2
EK1355-DA|Biotinylated anti- mouse TFPI antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
